8,432 results
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Plasmid#225223PurposeYeast optimized human Amyloid β42 fused with aga2 protein gene under gal1 promoter for the expression of Amyloid β42 in yeast surface display.DepositorTagsHA Tag and c-myc TagExpressionYeastPromoterGAL1Available sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only
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pLS406
Plasmid#231078PurposeMinimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker URA3DepositorPublicationTypeEmpty backboneUseIntegratingExpressionBacterial and YeastAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-GAL-Kan
Plasmid#232092PurposeYeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertSpCas9 and Ec86 reverse transcriptase
UseCRISPRExpressionYeastMutationEc86 reverse transcriptase is codon optimized for…PromoterGAL1-GAL10Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas3
Plasmid#229538PurposepMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.DepositorInsertCas3
UseCRISPRExpressionBacterial and YeastMutationcodon optimized for S.cerevisiaeAvailable sinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG144
Plasmid#227760PurposeExpresses a version of light-inducible transcription factor EL222 (Val121Met) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationVal121Met mutantPromoterPGK1prAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG124
Plasmid#227758PurposeExpresses a version of light-inducible transcription factor EL222 (Thr83Ser) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationThr83Ser mutantPromoterPGK1prAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOGS539_GLuc-ns
Plasmid#226719PurposePlasmid enabling yeast-mediated expression of Gaussia luciferase (GLuc)DepositorInsertGaussia Luciferase
TagsHA tagExpressionYeastPromoterpTEF1Available sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FRB-NLS-3HA-KanMX6
Plasmid#228022PurposeTo integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with KanMX6 markerDepositorInsertTurboID-FRB
TagsHA tagExpressionYeastAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
SJZ6-HIS5
Plasmid#228259PurposeTemplate plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3)DepositorInserthis5
ExpressionYeastPromoterTEF1 S.c.Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
CPY-ymsfGFP
Plasmid#209538PurposeExpresses CYP tagged with ymsfGFP in S. cerevisiaeDepositorInsertCPY
TagsymsfGFPExpressionYeastAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
CPY*-ymsfGFP
Plasmid#209539PurposeExpresses the misfolded CPY* mutant tagged with ymsfGFP in S. cerevisiaeDepositorInsertCPY*
TagsymsfGFPExpressionYeastAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FKBP-3HA-HIS3MX6
Plasmid#228025PurposeTo integrate TID-FKBP fusion at yeast promoter; yeast BioID with HIS3MX6 markerDepositorInsertTurboID-FKBP
TagsHA tagExpressionYeastAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FKBP-NLS-3HA-HISMX6
Plasmid#228027PurposeTo integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with HISMX6 markerDepositorInsertTurboID-FKBP
TagsHA tagExpressionYeastAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-ARG (V5 and HA)
Plasmid#222493PurposeCo-expression of two genes in yeast with V5 and HA tags and ARG1 selectionDepositorTypeEmpty backboneTagsHA and V5ExpressionYeastAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SJZ10-LEU2
Plasmid#228262PurposeTemplate plasmid for yeast gene knockout, uses auxotrophic marker LEU2 (Kluyveromyces lactis)DepositorInsertLEU2
ExpressionYeastPromoterLEU2 K.l.Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-TID-FKBP-HA3
Plasmid#227998PurposeExpresses TurboID-FKBP fusion from ADH1 promoter; yeast BioIDDepositorInsertTurboID-FKBP
TagsHA tagExpressionYeastPromoterADH1Available sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-HIS3p-TID-FKBP-HA3
Plasmid#228006PurposeExpresses TurboID-FKBP fusion from HIS3 promoter; yeast BioIDDepositorInsertTurboID-FKBP
TagsHA tagExpressionYeastPromoterHIS3Available sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC25A46[I349D]-His (SB256)
Plasmid#227605PurposeInducible expression of His-tagged SLC25A46 with I349D mutation in Pichia pastorisDepositorAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-Strep-Opa1 (SB251)
Plasmid#227600PurposeInducible expression of Strep-tagged Opa1 in Pichia pastorisDepositorInsertOPA1 (OPA1 Human)
TagsTwin-StrepTagExpressionYeastMutationcontains aa 253-960 onlyPromoterAOX1Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FKBP-3HA-KanMX6
Plasmid#228024PurposeTo integrate TID-FKBP fusion at yeast promoter; yeast BioID with KanMX6 markerDepositorInsertTurboID-FKBP
TagsHA tagExpressionYeastAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only