8,128 results

1. pCfB2400

   Plasmid

   #67552

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked dsdA marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Expression

   Yeast

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. POM43-mCitrine

   Plasmid

   #69855

   Purpose

   N-terminal tagging with mCitrine using LEU2 as a selection marker in S. cerevisiae.

   Depositor

   Liza Pon

   Article

   Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

   Insert

   mCitrine minus start and stop codon
   

   Expression

   Yeast

   Available Since

   April 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB3049(gRNA XII-4)

   Plasmid

   #73291

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-4

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB4929

   Plasmid

   #126751

   Purpose

   Expresses pTRX2_5xUAS-ALD6-tADH1 in yeast from plasdmid pRS413

   Depositor

   Michael Jensen

   Article

   Zhang et al ACS Synth Biol. 2016 Dec 16;5(12):1546-1

   Insert

   ALD6
   

   Expression

   Yeast

   Promoter

   pTRX2_5xUAS

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAW8-aid-eDSR-Turg1:kanMX6

   Plasmid

   #169362

   Purpose

   C-terminal aid-eDSR degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Expression

   Yeast

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAW8-aid-V5-eDSR-Turg1:kanMX6

   Plasmid

   #169365

   Purpose

   C-terminal aid-V5-eDSR degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Tags

   1XV5

   Expression

   Yeast

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAW8-aid-5Myc-Turg1:kanMX6

   Plasmid

   #169356

   Purpose

   C-terminal aid-5Myc degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Tags

   5XMyc

   Expression

   Yeast

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAW8-aid-3HA-eDSR-Turg1:kanMX6

   Plasmid

   #169363

   Purpose

   C-terminal aid-3HA-eDSR degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Tags

   3XHA

   Expression

   Yeast

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAW8-aid-5Myc-eDSR-Turg1:kanMX6

   Plasmid

   #169364

   Purpose

   C-terminal aid-5Myc-eDSR degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Tags

   5XMyc

   Expression

   Yeast

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGBW-m4046442

    Plasmid

    #145550

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. TEF1pro_mCherry_YFP

    Plasmid

    #138624

    Purpose

    Dual reporter backbone plasmid for CCRA experiment

    Depositor

    Rob Mitra

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. YCplac111-yVps23-mCherry

    Plasmid

    #45925

    Depositor

    James Hurley

    Article

    Ren et al EMBO J. 2011 Jun 1;30(11):2130-9. doi: 1

    Insert

    yeast Vps23 (STP22 Budding Yeast)
    

    Tags

    mCherry

    Expression

    Yeast

    Promoter

    lac

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pCfB2800

    Plasmid

    #63657

    Purpose

    pCfB2800 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Insert

    GFP
    

    Use

    Cre/Lox

    Expression

    Yeast

    Promoter

    TEF1 promoter

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDSM-de-Pfba1-PYC-Trpl15a

    Plasmid

    #127738

    Purpose

    Yeast pathway position 5. PYC transcription unit with the FBA1 promoter and RPL15A terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    Pfba1

    Available Since

    Jan. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK273

    Plasmid

    #71563

    Purpose

    Produces Crepis alpina delta12 fatty acid desaturase (FAD2-2) in yeast

    Depositor

    T. J. Kappock

    Article

    Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

    Insert

    delta12 fatty acid desaturase (form 2)
    

    Expression

    Bacterial and Yeast

    Promoter

    GAL1

    Available Since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDTN3997

    Plasmid

    #113337

    Purpose

    Multi-copy 2-pyrone synthase plasmid for triacetic acid lactone production in Yarrowia lipolytica

    Depositor

    Suzanne Sandmeyer

    Article

    Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

    Insert

    multi-copy 2-pyrone synthase
    

    Expression

    Yeast

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

17. 413-Met25 Kar2 TorsinA E171Q GFP

    Plasmid

    #32127

    Depositor

    Susan Lindquist

    Article

    Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

    Insert

    TorsinA (TOR1A Human)
    

    Tags

    GFP and Kar2

    Expression

    Yeast

    Mutation

    E171Q

    Available Since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pUR-sap1+

    Plasmid

    #15984

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Genetics. 2007 Feb . 175(2):553-66.

    Insert

    Sap1 (sap1 Fission Yeast)
    

    Expression

    Yeast

    Available Since

    Oct. 31, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pUDP123

    Plasmid

    #107269

    Purpose

    pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
    

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ube2m

    Plasmid

    #31423

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2M Human)
    

    Tags

    HA

    Expression

    Yeast

    Mutation

    N/A

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only