-
-
pDRF1-GW ymTurquoise2-T2A-mCherry
Plasmid#118442PurposeProduces equimolar expression of yeast codon-optimized mTq2 and mCherryDepositorInsertymTurquoise2-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIp ADE2-KanMx
Plasmid#122917PurposeADE2-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceMay 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTD26-S>A10-17
Plasmid#112826PurposepSMF2 with all S to A mutations in only repeats 10-17.DepositorInsertRPB1 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationpSMF2 with all S to A mutations in only repeats 1…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCTD26-S>A2-9
Plasmid#112825PurposepSMF2 with all S to A mutations in only repeats 2-9.DepositorInsertRPB1 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationpSMF2 with all S to A mutations in only repeats 2…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCTD26-S>A18-25
Plasmid#112827PurposepSMF2 with all S to A mutations in only repeats 18-25.DepositorInsertRPB1 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationpSMF2 with all S to A mutations in only repeats 1…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPTK008-3a-αMFΔ no Kex
Plasmid#84967PurposeType 3a, α-mating factor no Kex recognition site - secretion signalDepositorInsertα-mating factor no Kex recognition site secretion tag
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS423-SgH
Plasmid#163972PurposeHelper plasmid for cloning gRNAs under the control of the SNR52 promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-PAmKate-KanMX6
Plasmid#87028PurposeTag S. pombe genes in C terminal with PAmKateDepositorInsertPAmKate
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRP322
Plasmid#159303PurposeYeast tagging vector to create gene fusion with yeast codon optimized CitrineA206K under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationV2del, S3del, F64L, A206K, L231HPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW mRuby2-T2A-mTurquoise2
Plasmid#118429PurposeProduces equimolar expression of mRuby2 and mTq2DepositorInsertmRuby2-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPS1890
Plasmid#1362DepositorInsertNLS-ECFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRS406 ATG1
Plasmid#166846PurposeExpresses Atg1 with ATG1 3'UTR in yeasts.DepositorInsertATG1 (ATG1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterATG1 PromoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS-ATG1(406)
Plasmid#122068PurposeExpressing ATG1DepositorInsertATG1 (ATG1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDS247
Plasmid#34967DepositorInsertMid2(SS/TM)-GFP-LOVpep
UseTagsExpressionYeastMutationT406A, T407A, V416I, T–2SPromoterAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDS248
Plasmid#34968DepositorInsertMid2(SS/TM)-GFP-LOVpep
UseTagsExpressionYeastMutationT406A, T407A, V416IPromoterAvailable sinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS415-BUD32
Plasmid#10991DepositorInsertBUD32 (BUD32 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationWild typePromoterAvailable sinceDec. 29, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAG305GAL-ccdB-EYFP
Plasmid#14209DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPTK011-3a-SA-αMFΔ
Plasmid#84970PurposeType 3a, Serum albumin followed by αMFΔ - secretion signalDepositorInsertSerum albumin followed by αMFΔ secretion tag
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPAP001
Plasmid#153488PurposeLevel 1 episomal expression plasmid for P. pastorisDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only
