8,266 results

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pLyGo-Kp-2

Plasmid

#163144

Purpose

pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Promoter

AOX1

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRS306Sup35R1

Plasmid

#17715

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

pRS306Sup35R1 (SUP35 Budding Yeast)

Expression

Yeast

Mutation

An pRS306 based integrating plasmid designed for …

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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413-Met25 Kar2 TorsinADE GFP

Plasmid

#32120

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Tags

GFP and Kar2

Expression

Yeast

Mutation

deletion of E at 302 or 303

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pLEP1-H-Hsp82

Plasmid

#1277

Depositor

Susan Lindquist

Article

Chang et al J Biol Chem 1994 Oct 7;269(40):24983-8.

Insert

his-Hsp82 (HSP82 Budding Yeast)

Tags

Histidinex6

Expression

Yeast

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
pSP-GM2

Plasmid

#64740

Purpose

Yeast expression plasmid

Depositor

Jens Nielsen

Article

Chen et al FEMS Yeast Res. 2012 Aug;12(5):598-607.

Type

Empty backbone

Expression

Yeast

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only
pDS255

Plasmid

#34970

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

T406A, T407A

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pXRU3

Plasmid

#63152

Purpose

Ura3 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

Depositor

Marc Gartenberg

Article

Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

Type

Empty backbone

Use

Cre/Lox

Expression

Bacterial and Yeast

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pI4-MTU-DO-HIS

Plasmid

#160026

Purpose

Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Histidine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pI2-MTU-DO-HPH

Plasmid

#160019

Purpose

Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Hygromycin selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pI2-MTU-DO-LEU

Plasmid

#160017

Purpose

Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Leucine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-3YFP-kanMX6

Plasmid

#105149

Purpose

Yeast gene targeting

Depositor

David Kovar, Thomas Pollard, Jian-Qiu Wu

Article

Wu et al J Cell Biol. 2006 Jul 31;174(3):391-402.

Insert

3YFP-kanMX6

Tags

3YFP

Expression

Bacterial and Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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BS-Lys2

Plasmid

#69199

Purpose

complementation of yeast auxotrophic marker LYS2

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

LYS2

Expression

Yeast

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pJAID8Z

Plasmid

#165053

Purpose

Integrative expression of yEGFP and SKP1-fusing rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>SKP1>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pJAID6A

Plasmid

#165046

Purpose

Integrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>TAgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCSJ125

Plasmid

#104344

Purpose

Express yeast MDH2-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

Depositor

Alexander Varshavsky

Article

Chen et al Science. 2017 Jan 27;355(6323). pii: 355

Insert

MDH2

Tags

3xFLAG

Expression

Yeast

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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LD221

Plasmid

#69189

Purpose

Integration of 2GFP tag at Atg21 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg21-2EGFP

Tags

2xEGFP

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pXP316

Plasmid

#26836

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pRG236

Plasmid

#64534

Purpose

Integrative E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCK011

Plasmid

#105343

Purpose

Yeast two hybrid

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCK012

Plasmid

#105344

Purpose

Yeast two hybrid

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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