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pI4-MTU-DO-HIS

Plasmid

#160026

Purpose

Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Histidine selection.

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI763 p(Z)-mCherry + UBC9 sgRNA

Plasmid

#164918

Purpose

CEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNA

Depositor

Nicholas Ingolia

Publication

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

P(Z)-mcherry

Use

CRISPR

Expression

Yeast

Promoter

P(Z), synthetic promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI757 P(PGK1)-citrine P(HIS4)-mCherry

Plasmid

#164917

Purpose

Divergent promoter expression cassette, P(HIS4) expression with P(PGK1) normalizer

Depositor

Nicholas Ingolia

Publication

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

P(PGK1)-citrine and P(HIS4)-mCherry

Expression

Yeast

Promoter

PGK1 (yeast) and HIS4 (yeast)

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pICE TRP1-URA3

Plasmid

#122894

Purpose

TRP1-URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pICE LYS2-KanMx

Plasmid

#122899

Purpose

LYS2-KanMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pICE LYS2-NatMx

Plasmid

#122900

Purpose

LYS2-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pICE TRP1-HphMx

Plasmid

#122901

Purpose

TRP1-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRS423-TyrSgH

Plasmid

#163973

Purpose

Helper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoter

Depositor

Jiazhang Lian

Publication

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRS423-SgH

Plasmid

#163972

Purpose

Helper plasmid for cloning gRNAs under the control of the SNR52 promoter.

Depositor

Jiazhang Lian

Publication

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAGT572_Nemo

Plasmid

#153487

Purpose

Level 1 episomal expression plasmid for S. cerevisiae

Depositor

Sylvestre Marillonnet

Publication

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Type

Empty backbone

Expression

Yeast

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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RCP83

Plasmid

#163466

Purpose

Backbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFP

Depositor

Frank Albert

Publication

Renganaath et al Elife. 2020 Nov 12;9. pii: 62669. doi: 1

Inserts

Promoter fragment (Sharon2012_1922)
His3 minimal promoter
yeGFP

Expression

Bacterial and Yeast

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pWL89A mmPing20

Plasmid

#145788

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Publication

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

mmPing20

Expression

Yeast

Mutation

T162C, T258A, T287C, T300A, T304A, T310A, G372A

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAG413 GAL ORF1 Shuffle1 NLS

Plasmid

#145794

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Publication

Payero et al Journal of the South Carolina Academy of

Insert

ORF1 Shuffle1 NLS

Expression

Yeast

Mutation

Enhanced Nucleur Localization Signal

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAG425 GAL Pong TPase L418A, L420A

Plasmid

#145795

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock, Susan Wessler

Publication

Hancock et al Mob DNA. 2010 Feb 1;1(1):5. doi: 10.1186

Insert

Pong TPase L418A, L420A

Expression

Yeast

Mutation

L418A, L420A

Available since

Jan. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pCTCON2-BXG-2TAG

Plasmid

#158138

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

Depositor

James Van Deventer

Publication

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the first serine residue…

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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plIVMD164

Plasmid

#160936

Purpose

In vivo mRNA display construct for GFP

Depositor

Saeed Tavazoie

Publication

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-sfGFP-HIS tag, 3'UTR MS2 Stem Loop

Tags

HIS tag, 3'UTR MS2 Stem Loop

Expression

Yeast

Promoter

MET25

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pVD_85

Plasmid

#149604

Purpose

(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - XylR

Depositor

Michael Jensen

Publication

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

XylR

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Promoter

TEF1

Available since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCL28-decoder

Plasmid

#159450

Purpose

Insert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.

Depositor

Joerg Stelling

Publication

Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

Insert

PlexAtet-LexA-ER-B112-tCyc1

Use

Synthetic Biology

Expression

Yeast

Promoter

PlexAtet

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCL66-decoder

Plasmid

#159453

Purpose

Insert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.

Depositor

Joerg Stelling

Publication

Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

Insert

Pfus1mut-tetR-nls-malE-tCyc1

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfus1mut

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pI2-MTU-DO-HPH

Plasmid

#160019

Purpose

Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Hygromycin selection.

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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