8,060 results

1. pESC-LEU2-RAD30cmyc

   Plasmid

   #60821

   Purpose

   to overexpress yeast polymerase eta in yeast

   Depositor

   Youri Pavlov, Thomas Kunkel

   Article

   Pavlov et al Mutat Res. 2001 Jul 1;478(1-2):129-39.

   Insert

   RAD30 (RAD30 Budding Yeast)
   

   Use

   Tags

   c-myc

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pFA6a-6xGLY-VSV-G-kanMX6

   Plasmid

   #20772

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAV113.loxP

   Plasmid

   #63186

   Purpose

   pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PdarC1

   Plasmid

   #24835

   Depositor

   David Baker

   Article

   Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

   Insert

   PdarC1
   

   Use

   Tags

   HA and Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pNB785

   Plasmid

   #60164

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus-PEST
   

   Use

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   SIC1pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNB695

   Plasmid

   #60144

   Purpose

   Disintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   GrLuc
   

   Use

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Mutation

   Promoter

   MET17pr

   Available Since

   Dec. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNB697

   Plasmid

   #60146

   Purpose

   Disintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   RdLuc
   

   Use

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Mutation

   Promoter

   MET17pr

   Available Since

   Dec. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNB690

   Plasmid

   #60134

   Purpose

   Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   YeLuc
   

   Use

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Mutation

   Promoter

   MET17pr

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS406 ATG9-GFP S122D

   Plasmid

   #114667

   Purpose

   Expresses yeast ATG9 (S122D) under the endogenous promoter with a GFP C-terminal tag

   Depositor

   Daniel Klionsky

   Article

   Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

   Insert

   ATG9
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   changed serine 122 to aspartic acid

   Promoter

   ATG9

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAG413GAL-ccdB-DsRed

    Plasmid

    #14357

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    DsRed

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFC663

    Plasmid

    #27324

    Depositor

    Fred Cross

    Article

    Cross et al Genetics. 2007 Jul . 176(3):1541-55.

    Insert

    SIC1 (SIC1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Contains insertion relative to SGDB sequence of G…

    Promoter

    Available Since

    Feb. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCfB2801

    Plasmid

    #63656

    Purpose

    pCfB2801 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Insert

    GFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CIPK2-upGFP

    Plasmid

    #117863

    Purpose

    protein expression

    Depositor

    Geoffrey Chang

    Insert

    CIPK2-upGFP (CIPK2 Mustard Weed)
    

    Use

    Tags

    eGFP

    Expression

    Yeast

    Mutation

    Promoter

    AOX

    Available Since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pETCON-DnvLB16 M40R;W41H;H42S

    Plasmid

    #45125

    Depositor

    David Baker

    Article

    Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

    Insert

    DnvLB16 M40R;W41H;H42S
    

    Use

    Tags

    Aga2p for yeast surface display and c-myc epitope…

    Expression

    Yeast

    Mutation

    M40R;W41H;H42S to DnvLB16

    Promoter

    GAL

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJW1675

    Plasmid

    #112049

    Purpose

    CEN/ARS plasmid for GAL-inducible TSC13-V5-TEV-EGFP expression marked with URA

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    TSC13
    

    Use

    Tags

    V5-TEV-EGFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRBUBR1empty

    Plasmid

    #24505

    Depositor

    Alexander Varshavsky

    Article

    Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 8, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pYZ166

    Plasmid

    #98419

    Purpose

    Pombe Expression Vector - nmt41 promoter with Sp.his3 marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYZ167

    Plasmid

    #98420

    Purpose

    Pombe Expression Vector - nmt81 promoter with Sp.his3 marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYZ175

    Plasmid

    #98422

    Purpose

    Pombe Expression Vector - nmt41 promoter with Sp.lys9 marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYZ176

    Plasmid

    #98423

    Purpose

    Pombe Expression Vector - nmt81 promoter with Sp.his3 marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge