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pWS1837

Plasmid

#123058

Purpose

_-factor pheromone

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

_-factor [3-4a]

Expression

Yeast

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCuHA-ATG13[3SA](426)

Plasmid

#122076

Purpose

Expressing HA-ATG13[3SA]

Depositor

Daniel Klionsky

Publication

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Tags

3HA

Expression

Yeast

Mutation

Serine 646 to alanine; Serine 649 to alanine; Ser…

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-ATG1(406)

Plasmid

#122068

Purpose

Expressing ATG1

Depositor

Daniel Klionsky

Publication

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pWS939

Plasmid

#123051

Purpose

Pheromone responsive transcription factor

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste12 [3]

Expression

Yeast

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pWS627

Plasmid

#123064

Purpose

G___ bicistronic protein

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste4-2A-Ste18 [3-4a]

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS788

Plasmid

#123059

Purpose

_-factor protease

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Bar1 [3]

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS936

Plasmid

#123050

Purpose

G_ subunit for creating C-terminal transplants

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Gpa1 C-terminal Truncation Vector [3-4a]

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS625

Plasmid

#123046

Purpose

Adenosine GPCR

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

A2BR [3]

Expression

Yeast

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS067

Plasmid

#123044

Purpose

_-factor GPCR

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste2 [3]

Expression

Yeast

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRC246

Plasmid

#123038

Purpose

Core promoter

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pLEU2m [2b]

Expression

Yeast

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS484

Plasmid

#123032

Purpose

LexA UAS

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (2x) [2a]

Expression

Yeast

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS483

Plasmid

#123031

Purpose

LexA UAS

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (3x) [2a]

Expression

Yeast

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS482

Plasmid

#123030

Purpose

LexA UAS

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (4x) [2a]

Expression

Yeast

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS053

Plasmid

#123025

Purpose

Pheromone responsive promoter

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pFIG1 [2]

Expression

Yeast

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS060

Plasmid

#123024

Purpose

Pheromone responsive promoter

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pFUS1 [2]

Expression

Yeast

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-Dhh1-PA(D195A,E196A,S226A,T228A)(405)

Plasmid

#122067

Purpose

Expressing Dhh1-PA

Depositor

Daniel Klionsky

Publication

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

Dhh1-PA (DHH1 Budding Yeast)

Tags

PA

Expression

Yeast

Mutation

Aspartic acid 195 to alanine; Glutamic acid 196 t…

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW YPET-T2A-mTurquoise2

Plasmid

#118450

Purpose

Produces equimolar expression of YPET and mTq2

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

YPET-T2A-mTurquoise2

Expression

Yeast

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCuGFP-SpAtg17(416)

Plasmid

#122080

Purpose

Expressing S. pombe GFP-Atg17

Depositor

Daniel Klionsky

Publication

Nanji et al Autophagy. 2017;13(12):2018-2027. doi: 1

Insert

SpATG17 (atg17 Fission Yeast)

Tags

GFP

Expression

Yeast

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pESC/CURT_fluoB

Plasmid

#117997

Purpose

Expression of CURT_fluoB controlled by the Gal1 promoter

Depositor

Mathias Pribil

Publication

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CURT_fluoB

Use

Yeast/bacteria binary vector

Expression

Yeast

Promoter

Gal1

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pYES2.1-pomSua1

Plasmid

#107446

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Publication

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Sua1

Tags

V5-His

Expression

Yeast

Mutation

See depositor comments below.

Promoter

GAL1

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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