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8,430 results

Showing: 4201 - 4220 of 8430 results

  1. pNB787

    Plasmid
    #60152
    Purpose
    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.
    Depositor
    Nicolas Buchler
    Publication
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    LEU1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60152-plasmid-map-sequence-id-96317

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  2. pNB784

    Plasmid
    #60163
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.
    Depositor
    Nicolas Buchler
    Publication
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    SIC1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60163-plasmid-map-sequence-id-96372

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  3. pNB788

    Plasmid
    #60165
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.
    Depositor
    Nicolas Buchler
    Publication
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    RNR1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60165-plasmid-map-sequence-id-96374

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  4. pNB789

    Plasmid
    #60166
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.
    Depositor
    Nicolas Buchler
    Publication
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    RNR1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60166-plasmid-map-sequence-id-96375

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  7. pHOHO2_Hsp82viiA

    Plasmid
    #61708
    Purpose
    Yeast integration plasmid targeted to the HO loci with heterodimeric Hsp82. Digest with NotI prior to addition to yeast.
    Depositor
    Dan Bolon
    Publication
    Mishra et al Mol Cell. 2014 Jan 23;53(2):344-50. doi:
    Insert
    Hsp82viiA (HSP82 Budding Yeast)
    Use
    Yeast integration vector targeting inserts to the…
    Tags
    6xHis
    Expression
    Yeast
    Mutation
    Heteromeric coiled coil inserted after amino acid…
    Promoter
    TEF1
    Available since
    Feb. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    61708-plasmid-map-sequence-id-104523

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  9. pNB753

    Plasmid
    #60148
    Purpose
    Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.
    Depositor
    Nicolas Buchler
    Publication
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    GrLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    LEU1pr
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60148-plasmid-map-sequence-id-96305

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  12. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - delta Y

    Plasmid
    #60393
    Purpose
    Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal tyrosine (delta Y)
    Depositor
    Ron Vale
    Publication
    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do
    Insert
    TUB1 (TUB1 Synthetic, Budding Yeast)
    Expression
    Yeast
    Mutation
    internal 6xHis (see supplement figure 4)
    Promoter
    GAL
    Available since
    Jan. 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pNB754

    Plasmid
    #60157
    Purpose
    Green beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by LYS9 promoter.
    Depositor
    Nicolas Buchler
    Publication
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    GrLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    LYS9pr
    Available since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60157-plasmid-map-sequence-id-96349

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  15. pNB695

    Plasmid
    #60144
    Purpose
    Disintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).
    Depositor
    Nicolas Buchler
    Publication
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    GrLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    MET17pr
    Available since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60144-plasmid-map-sequence-id-96226

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  16. pNB697

    Plasmid
    #60146
    Purpose
    Disintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).
    Depositor
    Nicolas Buchler
    Publication
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    RdLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    MET17pr
    Available since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60146-plasmid-map-sequence-id-96253

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  18. p41Neo 1-F GW

    Plasmid
    #58545
    Purpose
    CEN vector with G418 resistance marker for single Gateway insertion
    Depositor
    Leonid Kruglyak
    Type
    Empty backbone
    Expression
    Yeast
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    58545-plasmid-map-sequence-id-90632

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  20. UASgal-A9-Pleum

    Plasmid
    #60343
    Purpose
    Contains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.
    Depositor
    Hal Alper
    Publication
    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884
    Insert
    promoter
    Use
    Synthetic Biology; Yeast synthetic hybrid promote…
    Expression
    Yeast
    Available since
    Nov. 6, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    60343-plasmid-map-sequence-id-96621

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Showing: 4201 - 4220 of 8430 results