8,418 results

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pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation

Plasmid

#58830

Purpose

Expresses GFP-Tagged and mutated Osh2 PH domain in yeast

Depositor

Tim Levine

Publication

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

2 x Osh2 PH domain (OSH2 Budding Yeast)

Tags

GFP and Myc

Expression

Yeast

Mutation

changed Lysine 307 to Glutamate and Arginine 309 …

Promoter

PHO5

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
p42Nat 3-F GW

Plasmid

#58557

Purpose

Multicopy with clonNat resistance marker for triple Gateway insertion

Depositor

Leonid Kruglyak

Type

Empty backbone

Expression

Yeast

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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p41Neo 3-F GW

Plasmid

#58554

Purpose

CEN vector with G418 resistance marker for triple Gateway insertion

Depositor

Leonid Kruglyak

Type

Empty backbone

Expression

Yeast

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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p41Nat 1-F GW

Plasmid

#58546

Purpose

CEN vector with clonNat resistance marker for single Gateway insertion

Depositor

Leonid Kruglyak

Type

Empty backbone

Expression

Yeast

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRS406-PHO5-GFP-CERT PH domain EE mutation

Plasmid

#58833

Purpose

Expresses GFP-Tagged and mutated CERT PH domain in yeast

Depositor

Tim Levine

Publication

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)

Tags

GFP and Myc

Expression

Yeast

Mutation

changed Arginine 43 to Glutamate

Promoter

PHO5

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pGLx2-set1deltaRRM

Plasmid

#53257

Purpose

GAL driven LexA-tagged SET1-deltaRRM (RRM domain deleted)

Depositor

Jean Cook

Publication

Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

Insert

SET1

Tags

LexA

Expression

Yeast

Mutation

RRM domain deleted (aa 230-355)

Promoter

GAL1/10

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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p416GAL1-Skn7t-L-IFP-F[1] (Yeast)

Plasmid

#52896

Purpose

Skn7t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Publication

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Skn7t-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pGLx2-SET1

Plasmid

#53256

Purpose

GAL driven LexA-tagged SET1

Depositor

Jean Cook

Publication

Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

Insert

SET1

Tags

LexA

Expression

Yeast

Promoter

GAL1/10

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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p416GAL1-Ssk1t-L-IFP-F[1] (Yeast)

Plasmid

#52897

Purpose

Ssk1t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Publication

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Ssk1t-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
M3939 leu2::ADE2 Disruptor Converter

Plasmid

#51672

Purpose

Yeast marker swap plasmid for converting LEU2 to ADE2

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

ADE2 (ADE2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Promoter

ADE2

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pCB116

Plasmid

#53365

Purpose

To express Cup9p‐FLAG from the PGAL1 promoter

Depositor

Alexander Varshavsky

Publication

Byrd et al EMBO J. 1998 Jan 2. 17(1):269-77.

Insert

Cup9 (CUP9 Budding Yeast)

Tags

Flag

Expression

Yeast

Promoter

GAL1

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX-tetO-Pcyc1-GST-LINKER

Plasmid

#52688

Purpose

Same as 41026, with additional linker

Depositor

Michael Nick Boddy

Insert

tetO7-Pcyc1-GST+LINKER

Use

Promoter replacement

Expression

Yeast

Available since

April 30, 2014

Availability

Academic Institutions and Nonprofits only

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p413TEF-Sir2-H364Y

Plasmid

#51743

Purpose

overexpression of Sir2 in S. cerevisiae (H364Y mutant), HIS3 marker

Depositor

Markus Ralser

Publication

Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

Insert

Sir2 (SIR2 Budding Yeast)

Expression

Yeast

Mutation

H364Y

Promoter

TEF1

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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p413TEF-CARKL

Plasmid

#51747

Purpose

overexpression of mouse Sedoheptulokinase (CARKL, SHPK) in yeast

Depositor

Markus Ralser

Publication

Kruger et al Antioxid Redox Signal. 2011 Jul 15;15(2)

Insert

SHPK (Shpk Mouse)

Expression

Yeast

Promoter

TEF1

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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p413GPD-hTPI Lys13Arg

Plasmid

#50722

Purpose

expression of the human TPI Lys13Arg allele in yeast

Depositor

Markus Ralser

Publication

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Expression

Yeast

Mutation

changed Lysine 13 to Argenine

Promoter

GPD

Available since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only

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p413GPD-hTPI Ile170Thr

Plasmid

#50721

Purpose

expression of the human TPI Ile170Thr allele in yeast

Depositor

Markus Ralser

Publication

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Expression

Yeast

Mutation

Isoleucine 170 to Threonine

Promoter

GPD

Available since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only

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p413GPD-hTPI Ile170Val

Plasmid

#50720

Purpose

expression of the human TPI Ile170Val allele in yeast

Depositor

Markus Ralser

Publication

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Expression

Yeast

Mutation

Isoleucine 170 to Valine

Promoter

GPD

Available since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pAG300

Plasmid

#19363

Depositor

Holger Deising

Publication

Horbach et al Plant Cell. 2009 Oct;21(10):3379-96. doi

Type

Empty backbone

Expression

Yeast

Available since

Dec. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pAAH3

Plasmid

#41827

Purpose

Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for phleomycin resistance.

Depositor

Melissa Moore

Publication

Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

Type

Empty backbone

Use

Pcr template

Expression

Yeast

Promoter

TEF

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pDR-AmTrac-100

Plasmid

#47771

Purpose

expresses AmTrac-100 in yeast

Depositor

Wolf Frommer

Publication

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac-100

Tags

mcpGFP

Expression

Yeast

Mutation

A141E, T464D

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only