8,060 results

1. pAG416-GAL-SUP35

   Plasmid

   #181705

   Purpose

   Galactose inducible expression of SUP35

   Depositor

   Alejandro Chavez

   Insert

   SUP35 (SUP35 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS413-TEF1pr-CFAPorig

   Plasmid

   #221092

   Purpose

   FAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3

   Plasmid

   #221112

   Purpose

   FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   MFA-IgKappa-FAPoptim-STE3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   STE3

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS415-TEF1pr-STE3-GFP

   Plasmid

   #221113

   Purpose

   GFP-tagged STE3 (C-terminally tagged, optimized for yeast expression)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   STE3-GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG415Gal_Gcn4 E3GFP

   Plasmid

   #233008

   Purpose

   Galactose iduced expression of Gcn4 E+GFP in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 E+
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   D105E, D118E, D139E

   Promoter

   Gal1

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Fyv6-NFLAG del1-51

   Plasmid

   #234136

   Purpose

   First 51 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminus

   Depositor

   Aaron Hoskins

   Insert

   Fyv6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   N-terminus truncation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS424 Prp8 S1584F

   Plasmid

   #234122

   Purpose

   Suppressor screen mutant in full-length Prp8 on a TRP plasmid

   Depositor

   Aaron Hoskins

   Insert

   Prp8
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   S1584F

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRS414-Syf1 817 Truncation

   Plasmid

   #234126

   Purpose

   Syf1 PCR truncation starting at 817 through the end of the gene

   Depositor

   Aaron Hoskins

   Insert

   Syf1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   N-terminus truncation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS424 Prp8 S1584Y

   Plasmid

   #234121

   Purpose

   Suppressor screen mutant in full-length Prp8 on a TRP plasmid

   Depositor

   Aaron Hoskins

   Insert

   Prp8
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   S1584Y

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRS424 Prp8 V1862L

    Plasmid

    #234123

    Purpose

    Suppressor screen mutant in full-length Prp8 on a TRP plasmid

    Depositor

    Aaron Hoskins

    Insert

    Prp8
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    V1862L

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAG415Gal_Gcn4 F124IHA

    Plasmid

    #232993

    Purpose

    Galactose iduced expression of Gcn4 F124IHA in yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 F124I
    

    Use

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    F124I

    Promoter

    Gal1

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAG415Gal_FL Gcn4

    Plasmid

    #232955

    Purpose

    Galactose iduced expression of full-length Gcn4 in yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Fyv6-NFLAG del1-16

    Plasmid

    #234138

    Purpose

    First 16 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminus

    Depositor

    Aaron Hoskins

    Insert

    Fyv6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N-terminus truncation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Fyv6-NFLAG del103-173

    Plasmid

    #234134

    Purpose

    Amino acids 103-173 deleted in Fyv6 with FLAG epitope tag at N-terminus

    Depositor

    Aaron Hoskins

    Insert

    Fyv6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C-terminus truncation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS414-Fyv6-CFLAG

    Plasmid

    #234133

    Purpose

    Restriction digest from pUC18-CFLAG-Fyv6 and ligation into pRS414

    Depositor

    Aaron Hoskins

    Insert

    Fyv6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Fyv6-NFLAG del134-173

    Plasmid

    #234135

    Purpose

    Amino acids 134-173 deleted in Fyv6 with FLAG epitope tag at N-terminus

    Depositor

    Aaron Hoskins

    Insert

    Fyv6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C-terminus truncation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS414-Fyv6

    Plasmid

    #234140

    Purpose

    Amplified Fyv6 from gDNA, cut with SalI and NotI, ligated into pRS414

    Depositor

    Aaron Hoskins

    Insert

    Fyv6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS414-NFLAG-Fyv6

    Plasmid

    #234139

    Purpose

    Restriction digest from pUC18-NFLAG-Fyv6 and ligation into pRS414

    Depositor

    Aaron Hoskins

    Insert

    Fyv6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS424 Prp8 G1868R

    Plasmid

    #234124

    Purpose

    Suppressor screen mutant in full-length Prp8 on a TRP plasmid

    Depositor

    Aaron Hoskins

    Insert

    Prp8
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    G1868R

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS416-Fyv6

    Plasmid

    #234141

    Purpose

    Amplified Fyv6 from gDNA, cut with SalI and NotI, ligated into pRS416

    Depositor

    Aaron Hoskins

    Insert

    Fyv6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge