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pYea5.1_Notch3_sfGFP

Plasmid

#207864

Purpose

Yeast 2µ vector for expression of Suc2SP-Notch3_100-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-Notch3_100-sfGFP-GAL4 fusion protein

Tags

Strep-TagII

Expression

Bacterial and Yeast

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pWR54

Plasmid

#203330

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHflu3

Plasmid

#203881

Purpose

pHI1 with insertion of kanamycin-resistance gene

Depositor

Bogumil Karas

Insert

aminoglycoside O-phosphotransferase APH(3')-IIa

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3243

Plasmid

#210187

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33P-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::aro7-R33P-GFP::tADH1 (ARO7 Budding Yeast)

Expression

Yeast

Mutation

R33P

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3217

Plasmid

#210185

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151M-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W151M-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W151M

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3186

Plasmid

#210181

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W353G-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W353G-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W353G

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3178

Plasmid

#210179

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::ade1-G54V-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::ade1-G54V-GFP::tADH1 (ADE2 Budding Yeast)

Expression

Yeast

Mutation

G54V

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3176

Plasmid

#210178

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-G141S-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::aro7-G141S-GFP::tADH1 (ARO7 Budding Yeast)

Expression

Yeast

Mutation

G141S

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3145

Plasmid

#210169

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-D147V-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::aro7-D147V-GFP::tADH1 (ARO7 Budding Yeast)

Expression

Yeast

Mutation

D147V

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3139

Plasmid

#210167

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33G-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::aro7-R33G-GFP::tADH1 (ARO7 Budding Yeast)

Expression

Yeast

Mutation

R33G

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3135

Plasmid

#210166

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-I245N-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-I245N-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

I245N

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3057

Plasmid

#210162

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-I36D-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-I36D-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

I36D

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3046

Plasmid

#210156

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151L-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W151L-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W151L

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3042

Plasmid

#210152

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151E-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W151E-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W151E

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3039

Plasmid

#210149

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151G-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W151G-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W151G

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3016

Plasmid

#210147

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-V26D-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-V26D-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

V26D

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3002

Plasmid

#210145

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W353R-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W353R-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W353R

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCOA-Leu-FvPPT1

Plasmid

#179923

Purpose

Expression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1

Depositor

Jens Laurids Sørensen

Article

Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

Insert

FvPPT1

Expression

Yeast

Mutation

Codon optimized for expression in Saccharomyces c…

Promoter

Yarrowia lipolytica EXP1

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3216

Plasmid

#210184

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151K-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W151K-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W151K

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3040

Plasmid

#210150

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151R-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W151R-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W151R

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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