8,126 results

1. pNSU299_SLA1

   Plasmid

   #166075

   Purpose

   Plasmid for constituive spCas9 and tet-inducible SLA1 targeting sgRNA expression for double stranded break formation in yeast

   Depositor

   Rebecca Burgess

   Insert

   SLA1 (SLA1 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   Tet-inducible

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNSU299_TDH3

   Plasmid

   #166084

   Purpose

   Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of TDH3 for double stranded break formation in yeast.

   Depositor

   Rebecca Burgess

   Insert

   Promoter of TDH3 (TDH3 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   Tet-inducible

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNSU299_PPE1

   Plasmid

   #166083

   Purpose

   Plasmid for constitutive spCas9 and tet-inducible PPE1 targeting sgRNA expression for double stranded break formation in yeast

   Depositor

   Rebecca Burgess

   Insert

   PPE1 (PPE1 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   Tet-inducible

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNSU299_PDR12

   Plasmid

   #166093

   Purpose

   Plasmid for constituive spCas9 and tet-inducible PDR12 targeting sgRNA expression for double stranded break formation in yeast

   Depositor

   Rebecca Burgess

   Insert

   PDR12 (PDR12 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   Tet-inducible

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNSU299_TDH2

   Plasmid

   #166082

   Purpose

   Plasmid for constituive spCas9 and tet-inducible TDH2 targeting sgRNA expression for double stranded break formation in yeast

   Depositor

   Rebecca Burgess

   Insert

   TDH2 (TDH2 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   Tet-inducible

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNSU299_LYS9

   Plasmid

   #166085

   Purpose

   Plasmid for constituive spCas9 and tet-inducible LYS9 targeting sgRNA expression for double stranded break formation in yeast

   Depositor

   Rebecca Burgess

   Insert

   LYS9 (LYS9 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   Tet-inducible

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pROS15_MATz

   Plasmid

   #166100

   Purpose

   Expression of dual gRNA targeting the MAT locus

   Depositor

   Rebecca Burgess

   Insert

   MAT Z1
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJT9RFR

   Plasmid

   #165067

   Purpose

   Introducing GAL promoters-controlled sequiterpene nerolidol-producing module and Y-FAST and in S. cerevisiae

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   T-RPL3>ERG20>P-GAL1-P-GAL2>Y-FAST>ErB1.2A-AcNES1>T-RPL41B
   

   Expression

   Yeast

   Mutation

   WT

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIR3DH8K

   Plasmid

   #165069

   Purpose

   Knocking out GAL80 using KanMX4 marker

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   gal80Arm1-P-TPI1-KanMX4-gal80Arm2
   

   Expression

   Yeast

   Mutation

   WT

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pVG97

    Plasmid

    #164849

    Purpose

    Plasmid for replacement of PEST sequence in strains with pr-yEVenus-PEST-ADH1t construct

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    Part of URA3 5' UTR and yEVenus with stop codon for recombination with pr-yEVenus-PEST-ADH1t:URA
    

    Expression

    Yeast

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIR3DH8

    Plasmid

    #165068

    Purpose

    Knocking out GAL80 using KlURA3 marker

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    gal80Arm1-P-AgTEF1-KlURA3-T-AgTEF1-gal80Arm2
    

    Expression

    Yeast

    Mutation

    WT

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pILGFP1H5

    Plasmid

    #165065

    Purpose

    Characterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-T-URA3
    

    Expression

    Yeast

    Mutation

    WT

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJAID5WY

    Plasmid

    #165050

    Purpose

    Integrative expression of SOD1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>SOD1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Expression

    Yeast

    Mutation

    OsTIR1 is codon-optimised for expression in S. ce…

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJAID6JY

    Plasmid

    #165049

    Purpose

    Integrative expression of TRX1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>TRX1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Expression

    Yeast

    Mutation

    WT

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RIB ONLY R3

    Plasmid

    #161511

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_RIB1_R3_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting R3 in the RIB1 promoter)
    

    Expression

    Yeast

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RIB ONLY R4

    Plasmid

    #161512

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_RIB1_R4_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting R4 in the RIB1 promoter)
    

    Expression

    Yeast

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. THI ONLY pALD4 T1

    Plasmid

    #161498

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T1_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T1 in the THI11 promoter)
    

    Expression

    Yeast

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. THI ONLY pALD4 T2

    Plasmid

    #161499

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T2_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T2 in the THI11 promoter)
    

    Expression

    Yeast

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. THI ONLY pALD4 T3

    Plasmid

    #161500

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T3_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T3 in the THI11 promoter)
    

    Expression

    Yeast

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. THI ONLY pALD4 T4

    Plasmid

    #161501

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T4_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T4 in the THI11 promoter)
    

    Expression

    Yeast

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge