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pABY-T1

Plasmid

#201764

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Expression

Yeast

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

Plasmid

#86421

Purpose

N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

CDC42pr-KAR2SS-GFP1-10

Tags

GFP1-10

Expression

Yeast

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-Strep-tagII-kanMX6

Plasmid

#20757

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available Since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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pTEF-Sup35C

Plasmid

#1202

Depositor

Susan Lindquist

Article

True et al Nature 2004 Sep 9;431(7005):184-7. Epub

Insert

Sup35 C-terminus (SUP35 Budding Yeast)

Expression

Yeast

Mutation

Sup35 C-terminus ~254aa-end

Promoter

TEF

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pAW212

Plasmid

#172196

Purpose

P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites

Depositor

Chang Liu

Article

Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

Insert

p10B2-AT110

Expression

Yeast

Promoter

10B2 (P1 specific promoter)

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pKP112 (pRS406-GFP-ADH1t)

Plasmid

#106472

Purpose

Vector for creating C-terminal GFP fusion proteins in yeast.

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Type

Empty backbone

Tags

GFP

Expression

Bacterial and Yeast

Promoter

None

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pRR-AR

Plasmid

#73045

Purpose

Constitutively expresses the androgen receptor, which is required for the activation of the promoter in pLAREG (Addgene plasmid #73045)

Depositor

David McMillen

Article

Mazumder et al Nucleic Acids Res. 2014 Aug;42(14):9514-

Insert

Androgen receptor (AR Human)

Expression

Yeast

Mutation

please see depositor comments below

Promoter

glyceraldehyde phosphate dehydrogenase (GPD)

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pMJA181

Plasmid

#128539

Purpose

Expresses anti-Prunus dulcis scFv Ab+BirA tag

Depositor

Marcos Alcocer

Article

de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

Insert

scFv Ab clone PD1F6 plus BirA tag

Expression

Yeast

Promoter

AOX1

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pRR-ERalpha-5Z

Plasmid

#23061

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

ERalpha (ESR1 Human)

Expression

Yeast

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only
pRS424GFP-FYVE(EEA1)

Plasmid

#36096

Depositor

Scott Emr

Article

Burd et al Mol Cell. 1998 Jul;2(1):157-62.

Insert

FYVE(EEA1) domain (EEA1 Human)

Tags

GFP

Expression

Yeast

Promoter

PRC1

Available Since

June 14, 2012

Availability

Academic Institutions and Nonprofits only

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pCfB4906

Plasmid

#106150

Purpose

EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying hygromycin resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

Cas9

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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PTEF1-yEGFPCLN2PEST-pRS406

Plasmid

#64406

Purpose

TEF promoter driven GFP expression

Depositor

Claudia Vickers

Article

Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

Insert

TEF1 promoter (TEF1 Budding Yeast)

Tags

yEGFPCLN2PEST (destabilized yeast EGFP)

Expression

Yeast

Promoter

TEF1 promoter

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pMJA179

Plasmid

#128537

Purpose

Expresses anti-Prunus dulcis scFv Ab

Depositor

Marcos Alcocer

Article

de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

Insert

scFv Ab clone PD1F6

Expression

Yeast

Promoter

AOX1

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pH-tdGFP

Plasmid

#74322

Purpose

pH stable tandem dimeric sfGFP variant (N149Y,Q204H) in pFA6a-URA3MX. Codon optimized for S. cerevisiae

Depositor

Joerg Stelling

Article

Roberts et al Sci Rep. 2016 Jun 21;6:28166. doi: 10.10

Insert

pH-tdGFP

Use

Yeast tagging

Tags

none

Expression

Yeast

Mutation

N149Y, Q204H

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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YIplac211-KAR2-msGFP2-HDEL

Plasmid

#160468

Purpose

Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to msGFP2 with an ER retrieval signal

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

KAR2-msGFP2-HDEL

Expression

Yeast

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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SZ_EC_02

Plasmid

#155341

Purpose

Overexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XR

Depositor

Jens Nielsen

Article

Konzock et al BMC Microbiol. 2021 Mar 8;21(1):77. doi:

Inserts

tef1 promoter - xylulose kinase - pex20 terminator
pyk1 promoter - xylose reductase - pex16 terminator
gapdh promoter - xylitol dehydrogenase - lip2 terminator

Use

Easycloneyali insert vector

Expression

Yeast

Promoter

gapdh, pyk1, and tef1

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pSC5

Plasmid

#188602

Purpose

Superior Conjugative Plasmid to Diverse Yeast

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW yAT1.03R122KR126K

Plasmid

#132782

Purpose

Non-binding version of the FRET biosensor for ATP measurements in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Sens. 2020 Mar 27;5(3):814-822. doi:

Insert

yAT1.03R122KR126K ymTq2-tdTomato

Expression

Yeast

Mutation

R122K, R126K

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pRS426 Gal TDP43

Plasmid

#27466

Depositor

Aaron Gitler

Article

Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

Insert

TDP-43 (TARDBP Human)

Expression

Yeast

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only
pJK-caCas9-NatMX-Neut5L-Ade2 drive

Plasmid

#89577

Purpose

caCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locus

Depositor

George Church

Article

Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

Insert

Ade2 gene drive

Use

Synthetic Biology

Expression

Yeast

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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