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pDRF1-GW AKAR3-EV

Plasmid

#182533

Purpose

Expresses the PKA FRET sensor AKAR3-EV in budding yeast

Depositor

Bas Teusink

Article

Botman et al FEMS Yeast Res. 2023 Jan 4;23:foad029. d

Insert

AKAR3-EV

Expression

Yeast

Promoter

PMA1

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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ZJOM82

Plasmid

#133648

Purpose

Integration of NAB2-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-NAB2-mCherry (NAB2 Budding Yeast)

Tags

mCherry

Expression

Yeast

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1513

Plasmid

#62383

Purpose

pRS316-pTDH3-Su9-TagBFP

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

su9-tagBFP

Tags

Su9 presequence

Expression

Yeast

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pYZ960

Plasmid

#187972

Purpose

TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2µ-URA3

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Promoter

TEF1

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pRS424GFP-FYVE(EEA1)

Plasmid

#36096

Depositor

Scott Emr

Article

Burd et al Mol Cell. 1998 Jul;2(1):157-62.

Insert

FYVE(EEA1) domain (EEA1 Human)

Tags

GFP

Expression

Yeast

Promoter

PRC1

Available Since

June 14, 2012

Availability

Academic Institutions and Nonprofits only

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pUDP004

Plasmid

#101165

Purpose

E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

Depositor

Jean-Marc Daran

Article

de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Promoter

ScTDH3

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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Leu2 2u

Plasmid

#220622

Purpose

empty backbone for shuttle vector construction

Depositor

Marvin Wickens

Article

Preston et al Nat Methods. 2019 May;16(5):437-445. doi

Insert

empty vector (LEU2 Budding Yeast)

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMVS142_pACT1_mCherry_Zif268_EBD_MCS_KAN

Plasmid

#99049

Purpose

Transcription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domain

Depositor

Barak Cohen

Article

Staller et al Cell Syst. 2018 Mar 1. pii: S2405-4712(1

Inserts

ACT1 promoter (ACT1 Budding Yeast)
mCherry
Zif268 DNA binding Domain
Estrogen response domain (ESR1 Human)

Expression

Yeast

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pBEVY-U

Plasmid

#51230

Purpose

bi-directional expression vector for yeast, constitutively active, URA3 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GPD/ADH1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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CUP1-∆ssCPY*-mScarletI in pRS315

Plasmid

#221159

Purpose

Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker

Depositor

Mark Leake

Insert

Carboxypeptidase Y (PRC1 Budding Yeast)

Tags

mScarletI

Expression

Yeast

Mutation

Deletion signal peptide, G255A mutation

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pFA CUP1p-NuiHA kanMX

Plasmid

#131169

Purpose

PCR template for genomic ScCUP1 promoter regulated N-terminal Nui-HA prey fusion, kanMX marker confers resistance to geneticin in S. cerevisiae

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

Type

Empty backbone

Tags

Nui-HA

Expression

Yeast

Available Since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pTH753-CEN-RLuc/min346maxCFLuc

Plasmid

#38219

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

The first 346 codons of the original FLuc gene we…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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yHS1-M7A

Plasmid

#159151

Purpose

Cytosolic labile heme reporter

Depositor

Amit Reddi

Article

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

yHS1-M7A

Expression

Yeast

Mutation

Mutated Met 7 of the cyt b562 module of yHS1 to A…

Promoter

GPD

Available Since

Sept. 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCfB2793

Plasmid

#64043

Purpose

pCfB2793 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter and none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)

Plasmid

#179002

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-mTID-HA3-HIS3MX6

Plasmid

#227983

Purpose

To insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID,HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

miniTurboID

Tags

HA tag

Expression

Yeast

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pPK611

Plasmid

#44541

Depositor

Michael Grunstein

Article

Kayne et al Cell. 1988 Oct 7;55(1):27-39.

Insert

hhf2

Expression

Yeast

Mutation

del (90-95)

Available Since

June 4, 2013

Availability

Academic Institutions and Nonprofits only
FRP718_PACT1(-1-520)-LexA-ER-haB42-TCYC1

Plasmid

#58431

Purpose

Expresses LexA-ER-B42 in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-B42

Expression

Yeast

Promoter

Act1 promoter (-520;1)

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pCT2-HRP

Plasmid

#44021

Depositor

Dane Wittrup

Article

Lipovsek et al Chem Biol. 2007 Oct;14(10):1176-85.

Insert

HRP

Tags

HA and c-myc

Expression

Yeast

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only
pNK5867

Plasmid

#219751

Purpose

pGAP-like vector encoding hispidin-synthase from Mycena citricolor under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - mcitHispS - tAOX

Expression

Yeast

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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