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BB3eH_14*

Plasmid

#98543

Purpose

empty BB3 for insertion and overexpression of 1 gene in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB3eH_AC

Plasmid

#98544

Purpose

empty BB3 for insertion and overexpression of 2 genes in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB3eH_AD

Plasmid

#98545

Purpose

empty BB3 for insertion and overexpression of 3 genes in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-mYFP

Plasmid

#105138

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Publication

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P3nmt1-mYFP

Tags

3nmt1-mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

3nmt1

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-3nmt1-mEOS3.2

Plasmid

#105166

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Publication

Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-4

Insert

kanMX6-3nmt1-mEOS3.2

Tags

3nmt1-mEOS3.2

Expression

Bacterial and Yeast

Promoter

3nmt1

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P81nmt1-mEGFP

Plasmid

#105143

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Publication

Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

Insert

kanMX6-P81nmt1-mEGFP

Tags

81nmt1-mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

81nmt1

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-HSE1-iGFPx6

Plasmid

#105259

Purpose

Plasmid for pop-in/pop-out replacement of Hse1 with Hse1-iGFPx6.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Hse1 portion (HSE1 Budding Yeast)

Tags

iGFPx6

Expression

Yeast

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-MUP1-iYFP

Plasmid

#105266

Purpose

Plasmid for pop-in/pop-out replacement of Mup1 with Mup1-iYFP.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Mup1 portion (MUP1 Budding Yeast)

Tags

iYFP

Expression

Yeast

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac204-iGFP-YPT52

Plasmid

#105279

Purpose

Plasmid for integration of iGFP-Ypt52 at TRP1.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Ypt52 (YPT52 Budding Yeast)

Tags

iGFP

Expression

Yeast

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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p415 Ret2LD2a Q146F

Plasmid

#75259

Purpose

Q146F mutation

Depositor

Blanche Schwappach

Publication

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD2a

Tags

His

Expression

Yeast

Mutation

Q146F

Promoter

Met25

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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p415 Ret2LD2a T158K

Plasmid

#75262

Purpose

T158K mutation

Depositor

Blanche Schwappach

Publication

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD2a T158K

Tags

His

Expression

Yeast

Promoter

Met25

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p415 Ret2LD2a K153Q

Plasmid

#75261

Purpose

K153Q mutation

Depositor

Blanche Schwappach

Publication

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD2a K153Q

Tags

His

Expression

Yeast

Promoter

Met25

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p415 Ret2LD2a N152T

Plasmid

#75260

Purpose

N152T mutation

Depositor

Blanche Schwappach

Publication

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD2a N152T

Tags

His

Expression

Yeast

Promoter

Met25

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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P415 δCLD+Ret2 2α

Plasmid

#73878

Purpose

Swapped helices

Depositor

Blanche Schwappach

Publication

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

δCLD+Ret2 2α

Tags

His

Expression

Yeast

Promoter

Met25

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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P415 Ret2LD+δC2α

Plasmid

#73877

Purpose

Swapped helices

Depositor

Blanche Schwappach

Publication

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD+δC2α

Tags

His

Expression

Yeast

Promoter

Met25

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p415 δCLD

Plasmid

#73876

Purpose

δCLD - His tagged (2α helices+μHD deleted)

Depositor

Blanche Schwappach

Publication

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

δCLD

Tags

His

Expression

Yeast

Promoter

Met25

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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P415 Ret2LD

Plasmid

#73875

Purpose

Ret2LD - His tagged (2α helices+μHD deleted)

Depositor

Blanche Schwappach

Publication

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD

Tags

His

Expression

Yeast

Promoter

Met25

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p415 δCLD2α

Plasmid

#73874

Purpose

δCLD2α - His tagged (μHD deleted)

Depositor

Blanche Schwappach

Publication

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Arcn1/ δCLD2α (ARCN1 Bovine)

Tags

His

Expression

Yeast

Promoter

Met25

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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P415 Ret2LD2α

Plasmid

#73873

Purpose

Ret2LD2α - His tagged (μHD deleted)

Depositor

Blanche Schwappach

Publication

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD2α

Tags

His

Expression

Yeast

Promoter

Met25

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p415 Ret2 I155A

Plasmid

#73888

Purpose

I155A mutation

Depositor

Blanche Schwappach

Publication

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2 I155A

Expression

Yeast

Mutation

Ile 155 Ala

Promoter

Met25

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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