8,086 results
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Plasmid#17459Purposemodified pENTR vector for N-terminal ECFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsECFPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only
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Linked Free ClLIS1 (M)
Plasmid#182487PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertFPPS(M)-LS
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHCT2
Plasmid#130970PurposeHyPr plasmid with natMX markerDepositorInsertempty backbone
UseTagsExpressionYeastMutationPromoterAvailable SinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHMK34
Plasmid#130971PurposeHyPr plasmid with hphMX markerDepositorInsertempty backbone
UseTagsExpressionYeastMutationPromoterAvailable SinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
CUP1-∆ssCPY*-mGFP in pRS315
Plasmid#221158PurposeFluorescent reporter for cytoplasmic aggregates under LEU2 selection markerDepositorInsertCarboxypeptidase Y (PRC1 Budding Yeast)
UseTagsmGFPExpressionYeastMutationDeletion signal peptide, G255A mutationPromoterAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
CUP1-∆ssCPY*-mScarletI in pRS315
Plasmid#221159PurposeFluorescent reporter for cytoplasmic aggregates under LEU2 selection markerDepositorInsertCarboxypeptidase Y (PRC1 Budding Yeast)
UseTagsmScarletIExpressionYeastMutationDeletion signal peptide, G255A mutationPromoterAvailable SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-Gal-PA50
Plasmid#84902Purposeinduce expression of dipeptide repeat PA in yeastDepositorAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAS9cd
Plasmid#141252PurposeConstitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterTEF1 (Cas9), pSNR52 (gRNA)Available SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHS3
Plasmid#81038PurposeHO endonuclease expressionDepositorInsertNATMX - HO endonuclease
UseTagsExpressionYeastMutationPromoterTEFAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
2015-N iFAST
Plasmid#209415PurposeiFAST expression plasmid using MET3pDepositorInsertiFAST
UseTagsExpressionYeastMutationPromoterMET3Available SinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
p416 25Q GPD
Plasmid#1177DepositorAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGS_107
Plasmid#160650PurposeExpresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoterDepositorInsertApolipoprotein E e4 (APOE Human)
UseTagsKar2 signal sequenceExpressionYeastMutatione4 allelePromoterGAL1Available SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8
Plasmid#207031PurposeTet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.DepositorInsertPho8
UseTagsmRuby3ExpressionYeastMutationPromoterAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRL_CT_GI_del1
Plasmid#98744PurposeFor red/far-red light-regulated gene expression in Saccharomyces cerevisiae. Integrates into the yeast ura3-52 locus. Encodes constitutively expressed PhyBNT-synTALE-DBD and PIF3-NLS-VP64AD fusions.DepositorUseSynthetic BiologyTagsSV40-NLS, VP64-AD, and synTALE-DBDExpressionYeastMutationmutated nucleotide 576 C to A, deleted amino acid…PromoterFBA1 and TDH3Available SinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDS2142
Plasmid#184273PurposeTet-off genome insertion systemDepositorInsertCatTA
UseTagsExpressionYeastMutationPromoterTDH3Available SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPMVAd3
Plasmid#98298PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
UseTagsExpressionYeastMutationPromoterAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPMVAu8
Plasmid#98297PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL4A-EfmvaS-TEFM1-PRPL15A-EfmvaE -TEBS1
UseTagsExpressionYeastMutationPromoterAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TID-HA3-KanMX6
Plasmid#227980PurposeTo insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, KanMX6 markerDepositorInsertTurboID
UseTagsHA tagExpressionYeastMutationPromoterAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS403-HIS3-pINO4-SV40NLS-PfV-GS-Sapphire
Plasmid#203658PurposeYeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm GEMs under the HIS3 promoter.DepositorArticleInsertPfV
UseTagsSapphireExpressionYeastMutationPromoterAvailable SinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoEGFP-SpHis5
Plasmid#44836PurposeYeast tagging vector to create gene fusion with yeast optimized EGFP with SpHis5 selectionDepositorInsertyoEGFP
UseTagsExpressionYeastMutationPromoterAvailable SinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only