8,086 results
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Plasmid#203882PurposepHI1 with insertion of monomeric red fluorescent protein (mRFP)DepositorInsertmonomeric red fluorescent protein
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable SinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRG207
Plasmid#64530PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-tdTomato-SpHis5
Plasmid#44640PurposeYeast tagging vector to create gene fusion with tdTomato with SpHis5 selectionDepositorInserttdTomato
UseTagsExpressionYeastMutationPromoterAvailable SinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
UASGAL-Pgal
Plasmid#60338PurposeContains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoterDepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable SinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSSa26
Plasmid#186784PurposeExpression of reverse transactivator (CgrtTA)DepositorInsertCgrtTA
UseTagsExpressionYeastMutationPromoterScPGK1pAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS316-RGR-GFP
Plasmid#51056PurposeExpress self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.DepositorInsertRGR-GFP
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterYeast ADH1 promoterAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJT9R
Plasmid#98305PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3AcNES1>TRPL41B
UseTagsExpressionYeastMutationPromoterAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIp TRP1-HIS3
Plasmid#122912PurposeTRP1-HIS3 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable SinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-Z3-Nat
Plasmid#232100PurposeYeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-Z3-Hyg
Plasmid#232095PurposeYeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertZ3 promoter and Z3EV transcription factor
UseCRISPRTagsExpressionYeastMutationPromoterZ3Available SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Nat-ADE2
Plasmid#232103PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWS625
Plasmid#123046PurposeAdenosine GPCRDepositorInsertA2BR [3]
UseTagsExpressionYeastMutationPromoterAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEK36
Plasmid#210598PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter
UseTagsmClover3ExpressionBacterial and YeastMutationPromoterSpH2BproAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pE2c
Plasmid#17462Purposemodified pENTR vector for C-terminal 3x HA tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTags3xHAExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-HALO-URA
Plasmid#188932PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-HALO-URA
UseTagsExpressionYeastMutationPromoterAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGAN230
Plasmid#131122Purposereverse yTRAP, RNQ1-aTF reporting on tetR-mKate2DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterSUP35Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
BB3cN_pGAP_23*_pLAT1_Cas9
Plasmid#104906PurposehCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTCDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceFeb. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJW1662
Plasmid#112050PurposeC-terminal tag with - AID*(AtIAA17_71-114)-HA and OsTIR1 expression cassette marked with LEUDepositorInsertsIAA17
TIR1
UseTagsHAExpressionYeastMutationonly amino acids 71-114PromoterADH1 and NoneAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV0765
Plasmid#133501PurposeExpression of mCherry targeted to the nucleus with one NLS signalsDepositorInsertpLys3(BstZ17I)-p(tdh1)-NLSSV40-mCherry-terminator(ScADH1)-kanMX
UseTagsExpressionYeastMutationPromoterAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA-GFP-Ubc9ts
Plasmid#20369DepositorInsertSUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)
UseYeast/bacteria shuttleTagsGFPExpressionYeastMutationY68L This mutation renders Ubc9 temperature-sens…PromoterAvailable SinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only