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pAG415Gal_Gcn4 Aro2+

Plasmid

#232982

Purpose

Galactose iduced expression of Gcn4 Aro2+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro2+

Tags

TEV cleavage site

Expression

Yeast

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro3+

Plasmid

#232983

Purpose

Galactose iduced expression of Gcn4 Aro3+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

TEV cleavage site

Expression

Yeast

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 W120AHA

Plasmid

#232992

Purpose

Galactose iduced expression of Gcn4 W120AHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 W120A

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

W120A

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 SATtoG KtoRHA

Plasmid

#232994

Purpose

Galactose iduced expression of Gcn4 SATtoG KtoRHAin yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 SATtoG KtoR

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro3+HA

Plasmid

#232990

Purpose

Galactose iduced expression of Gcn4 Aro3+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_DBDHA

Plasmid

#232987

Purpose

Galactose iduced expression of Gcn4 DBDAHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

DBD

Tags

3xHA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WT 44merHA

Plasmid

#232988

Purpose

Galactose iduced expression of Gcn4 WT 44merHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WT 44mer

Tags

3xHA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro2+HA

Plasmid

#232989

Purpose

Galactose iduced expression of Gcn4 Aro2+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro2+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 F124IGFP

Plasmid

#233005

Purpose

Galactose iduced expression of Gcn4 F124IGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 F124I

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

F124I

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WW+HA

Plasmid

#232991

Purpose

Galactose iduced expression of Gcn4 WW+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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