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pAG415Gal_Gcn4 Swap3

Plasmid

#232960

Purpose

Galactose iduced expression of Gcn4 k0.47 in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 k0.47

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 R2

Plasmid

#232970

Purpose

Galactose iduced expression of Gcn4 R+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R+

Tags

TEV cleavage site

Expression

Yeast

Mutation

K118R, K140R

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 E+

Plasmid

#232963

Purpose

Galactose iduced expression of Gcn4 E+in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E+

Tags

TEV cleavage site

Expression

Yeast

Mutation

D105E, D118E, D139E

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 W120A

Plasmid

#232962

Purpose

Galactose iduced expression of Gcn4 W120A in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 W120A

Tags

TEV cleavage site

Expression

Yeast

Mutation

W120A

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 P+

Plasmid

#232971

Purpose

Galactose iduced expression of Gcn4 Proline in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Proline

Tags

TEV cleavage site

Expression

Yeast

Mutation

S122P, T131F, A141P, E143D

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 R3

Plasmid

#232973

Purpose

Galactose iduced expression of Gcn4 R++ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R++

Tags

TEV cleavage site

Expression

Yeast

Mutation

D103R, D133R, K143R

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Swap3GFP

Plasmid

#233010

Purpose

Galactose iduced expression of Gcn4 k0.47GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 k0.47

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoED WHA

Plasmid

#232995

Purpose

Galactose iduced expression of Gcn4 ILVtoED W+HAin yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED W+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

N126W, I128E, V130E, V135E, L137D, I142D

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 9acidAHA

Plasmid

#232997

Purpose

Galactose iduced expression of Gcn4 9acidAHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 9acidA

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_DBD

Plasmid

#232985

Purpose

Galactose iduced expression of Gcn4 DBD in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 DBD

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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