8,086 results

1. pNK5785

   Plasmid

   #219753

   Purpose

   pGAP-like vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of GAP promoter, for yeast expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pGAP - nnLuz_v4 - tAOX
   

   Use

   Luciferase

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYSD313

   Plasmid

   #212931

   Purpose

   Encodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   Sed1 (SED1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGADT7-ADH700-yeCherry-p150-yeGFP-DHFR

   Plasmid

   #24378

   Depositor

   Thomas Wandless

   Article

   Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

   Insert

   pADH1 yeCherry::p150yeGFP-DHFR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   1. Truncated version of ADH700 promoter (SbfI/Spe…

   Promoter

   Available Since

   May 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. 12URA-B-ScTop2∆CTD

   Plasmid

   #214924

   Purpose

   Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

   Depositor

   James Berger

   Article

   Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

   Insert

   topoisomerase II
   

   Use

   Tags

   6x His-TEV

   Expression

   Yeast

   Mutation

   Deleted amino acids 1178-1428

   Promoter

   Gal 1/10

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-link-yoSuperfolderGFP-SpHis5

   Plasmid

   #44837

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized SuperfolderGFP with SpHis5 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoSuperfolderGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYX233-b2delta-DHFR

   Plasmid

   #163760

   Purpose

   Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)delta(19)-DHFR

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   b2delta-DHFR (Dhfr Mouse, Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

   Promoter

   GAL1

   Available Since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXP118

   Plasmid

   #26825

   Depositor

   Nancy DaSilva, Suzanne Sandmeyer

   Article

   Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Dec. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNB2176

   Plasmid

   #113395

   Purpose

   Ty3-IN-GFP; alpha factor inducible

   Depositor

   Suzanne Sandmeyer

   Article

   Beliakova-Bethell et al RNA. 2006 Jan;12(1):94-101. doi: 10.1261

   Insert

   Ty3 with integrase-green fluorescent protein
   

   Use

   Tags

   IN-GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS402-GPD-GFP(S65T)

   Plasmid

   #85190

   Purpose

   Integrating plasmid, GFP(S65T) under GPD promoter

   Depositor

   David Bartel

   Article

   Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

   Insert

   EGFP
   

   Use

   Integrating, shuttle vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available Since

   Dec. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-mScarlet-I-mNeonGreen-kanMX (pKBJ007)

    Plasmid

    #173454

    Purpose

    Contains mScarletI-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mScarlet-I-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRG706_lexO-SbfI_LexA-TF_LEU2MX

    Plasmid

    #154818

    Purpose

    Inducible expression of SbfI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    SbfI endonuclease
    LexA-ER-B112
    

    Use

    Tags

    FLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDUP3

    Plasmid

    #92174

    Purpose

    Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by duplication.

    Depositor

    Judith Berman

    Article

    Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

    Insert

    NAT1
    

    Use

    Genbank: u03440.1

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-X0

    Plasmid

    #115565

    Purpose

    Control vector expressing GFP alone without any consensus repeats

    Depositor

    Michael Downey

    Article

    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

    Insert

    Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-link-yEmCFP-Hygro

    Plasmid

    #44644

    Depositor

    Kurt Thorn

    Insert

    yEmCFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    A206R

    Promoter

    Available Since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPS3858

    Plasmid

    #113393

    Purpose

    galactose-inducible Ty3-his3AI; additional PPT

    Depositor

    Suzanne Sandmeyer

    Insert

    Ty3-his3AI
    

    Use

    Tags

    his3AI

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG426-GAL-Alpha-synuclein-E46K

    Plasmid

    #181723

    Purpose

    Galactose inducible expression of Alpha-synuclein-E46K

    Depositor

    Alejandro Chavez

    Insert

    Alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    E46K

    Promoter

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. 416Gal-FUS-YFP

    Plasmid

    #29593

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pMVS142_pACT1_mCherry_Zif268_EBD_MCS_KAN

    Plasmid

    #99049

    Purpose

    Transcription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domain

    Depositor

    Barak Cohen

    Article

    Staller et al Cell Syst. 2018 Mar 1. pii: S2405-4712(1

    Inserts

    ACT1 promoter (ACT1 Budding Yeast)
    mCherry
    Zif268 DNA binding Domain
    Estrogen response domain (ESR1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGKB

    Plasmid

    #85077

    Purpose

    expression in Pichia pastoris

    Depositor

    Sheng Yang

    Article

    Yang et al Yeast. 2014 Apr;31(4):115-25.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAP

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFA6a-yomRuby3-SpHIS5

    Plasmid

    #204287

    Purpose

    For C-terminal tagging using yeast optimized mRuby3

    Depositor

    Linda Huang

    Article

    Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

    Insert

    pFA6-yomRuby3-SpHIS5
    

    Use

    Tags

    yomRuby3

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available Since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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