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ZJOM18

Plasmid

#133631

Purpose

Integration of NAB2-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast nucleus.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-NAB2-GFP (NAB2 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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p3HR2

Plasmid

#220738

Purpose

Component of yeast three hybrid system

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pWR76

Plasmid

#202776

Purpose

episomal fluorescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCherry
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-HALO-3xHA

Plasmid

#188937

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-HALO-3xHA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-HDEL

Plasmid

#129098

Purpose

For labeling endoplasmic reticulum (ER) in S. cerevisiae with GFP-HDEL

Depositor

Wei-Lih Lee

Article

Omer et al Elife. 2018 Aug 7;7. pii: 36745. doi: 10

Insert

TEF1p-SS-3xGlyAla-GFP-HDEL

Expression

Yeast

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pRR-ERbeta-5Z

Plasmid

#23062

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

ERbeta (ESR2 Human)

Expression

Yeast

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only
bRA90

Plasmid

#100951

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. LEU2 marker

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pDR FLIP43 GPS1

Plasmid

#101084

Purpose

GA biosensor expression plasmid

Depositor

Wolf Frommer, Alexander Jones

Article

Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

Insert

GPS1

Tags

6HIS and cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pJK-caCas9-NatMX-Neut5L-Ade2 drive

Plasmid

#89577

Purpose

caCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locus

Depositor

George Church

Article

Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

Insert

Ade2 gene drive

Use

Synthetic Biology

Expression

Yeast

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

Plasmid

#86421

Purpose

N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

CDC42pr-KAR2SS-GFP1-10

Tags

GFP1-10

Expression

Yeast

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pRY003

Plasmid

#81043

Purpose

HO endonuclease expression

Depositor

John McCusker

Insert

HO

Expression

Yeast

Promoter

endogenous

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
pMTU-DO-LEU

Plasmid

#160006

Purpose

Low-copy expression vector for K. marxianus, leucine selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pVB3011

Plasmid

#113389

Purpose

Galactose-inducible L-BC gag: cure killer

Depositor

Suzanne Sandmeyer

Article

Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528.

Insert

L-BC Gag

Expression

Yeast

Mutation

Please see depositor comments

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAG306GPD-ccdB

Plasmid

#14140

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-mCherry-SpHis5

Plasmid

#44643

Purpose

Yeast tagging vector to create gene fusion with mCherry with SpHis5 selection

Depositor

Kurt Thorn

Insert

mCherry

Expression

Yeast

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pTA-HUH

Plasmid

#221152

Purpose

HisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolytica

Depositor

Sheng Yang

Article

Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

Insert

HisG-URA3-HisG

Expression

Bacterial and Yeast

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pKT-iRFP-KAN

Plasmid

#64687

Purpose

pKT vector encoding yeast-codon optimized infrared fluorescent protein iRFP.

Depositor

Erin O'Shea

Article

Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

Insert

iRFP

Expression

Yeast

Mutation

Nucleotide sequence has been yeast-codon optimize…

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pIIIA/MS2-2

Plasmid

#220632

Purpose

For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMJA181

Plasmid

#128539

Purpose

Expresses anti-Prunus dulcis scFv Ab+BirA tag

Depositor

Marcos Alcocer

Article

de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

Insert

scFv Ab clone PD1F6 plus BirA tag

Expression

Yeast

Promoter

AOX1

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pPS2037

Plasmid

#1370

Depositor

Pamela Silver

Article

Brodsky et al RNA 2000 Dec;6(12):1737-49.

Insert

PGK1-U1A-PGK1 3' UTR (PGK1 Budding Yeast)

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only