8,086 results

1. pESC-TYR

   Plasmid

   #222484

   Purpose

   Co-expression of two genes in yeast with FLAG and MYC tags and TYR1 selection

   Depositor

   Kiyofumi Yamada, Tetsuo Matsuzaki

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   FLAG and Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pESC-MET (V5 and HA)

   Plasmid

   #222490

   Purpose

   Co-expression of two genes in yeast with V5 and HA tags and MET15 selection

   Depositor

   Kiyofumi Yamada, Tetsuo Matsuzaki

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   HA and V5

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pESC-LYS (V5 and HA)

   Plasmid

   #222491

   Purpose

   Co-expression of two genes in yeast with V5 and HA tags and LYS2 selection

   Depositor

   Kiyofumi Yamada, Tetsuo Matsuzaki

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   HA and V5

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pESC-TYR (V5 and HA)

   Plasmid

   #222492

   Purpose

   Co-expression of two genes in yeast with V5 and HA tags and TYR1 selection

   Depositor

   Kiyofumi Yamada, Tetsuo Matsuzaki

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   HA and V5

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pESC-THR (V5 and HA)

   Plasmid

   #222494

   Purpose

   Co-expression of two genes in yeast with V5 and HA tags and THR1 selection

   Depositor

   Kiyofumi Yamada, Tetsuo Matsuzaki

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   HA and V5

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pESC-THR

   Plasmid

   #222479

   Purpose

   Co-expression of two genes in yeast with FLAG and MYC tags and THR1 selection

   Depositor

   Kiyofumi Yamada, Tetsuo Matsuzaki

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   FLAG and Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pESC-ADE

   Plasmid

   #222480

   Purpose

   Co-expression of two genes in yeast with FLAG and MYC tags and ADE2 selection

   Depositor

   Kiyofumi Yamada, Tetsuo Matsuzaki

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   FLAG and Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pESC-ARG

   Plasmid

   #222481

   Purpose

   Co-expression of two genes in yeast with FLAG and MYC tags and ARG1 selection

   Depositor

   Kiyofumi Yamada, Tetsuo Matsuzaki

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   FLAG and Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ymTq2-yeVenus pDRF1-GW

   Plasmid

   #168076

   Purpose

   FRET fusion between ymTq2 and yeVenus for use in yeast

   Depositor

   Bas Teusink

   Insert

   ymTq2-yeVenus
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-SNAP-KanMX6

    Plasmid

    #87024

    Purpose

    Tag S. pombe genes in C terminal with SNAP

    Depositor

    Julien Berro

    Article

    Lacy et al Mol Biol Cell. 2017 Jun 28. pii: mbc.E17

    Insert

    SNAP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBEVY-T

    Plasmid

    #51232

    Purpose

    bi-directional expression vector for yeast, constitutively active, TRP1 selection

    Depositor

    Charles Miller

    Article

    Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD/ADH1

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVG122

    Plasmid

    #211765

    Purpose

    Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    strongLOV
    

    Use

    For single-copy integration (if cut with pmei)

    Tags

    SV40 NLS and VP16

    Expression

    Yeast

    Mutation

    compared to EL222, strongLOV contains Glu84Asp mu…

    Promoter

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCfB8786

    Plasmid

    #126920

    Purpose

    Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG426GAL-EGFP

    Plasmid

    #166143

    Purpose

    EGFP expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG426GAL-mRuby3

    Plasmid

    #166144

    Purpose

    mRuby3 expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    mRuby3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-ATG8(414)/GFP-AUT7(414)

    Plasmid

    #49424

    Purpose

    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.

    Depositor

    Daniel Klionsky

    Article

    Abeliovich et al Mol Biol Cell. 2003 Feb;14(2):477-90.

    Insert

    autophagy-related 8 (ATG8 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    ATG8

    Available Since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pRS306-pHIS3-2xGFP-tURA3

    Plasmid

    #116936

    Purpose

    Yeast integrative vector for the expression of 2xGFP

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    2xGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    HIS3

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. BS-Ura3Kl

    Plasmid

    #69195

    Purpose

    complementation of yeast auxotrophic marker URA3(Kluyveromyces lactis)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Kluyveromyces lactis URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTH733-2µ-RLuc/maxCFLuc

    Plasmid

    #40608

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS-SKII-URA-GAL1-fSNAP-3xHA

    Plasmid

    #188935

    Purpose

    Integration cassette to create N-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    URA-GAL1-fSNAP-3xHA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge