8,057 results

1. pNXRVc-HACre

   Plasmid

   #78400

   Purpose

   constitutive Cre expression vector

   Depositor

   Kazunori Tomita

   Article

   Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

   Inserts

   3xHA-nls-Cre
   natMX6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. p426 103Q GPD

   Plasmid

   #1184

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pRMH122-LexA-CRY2PHR

   Plasmid

   #40613

   Purpose

   Expresses LexA-CRY2PHR for light-inducible protein expression when coupled with VP16-CIB

   Depositor

   Chandra Tucker

   Article

   Hughes et al Methods. 2012 Aug 15.

   Insert

   LexA-CRY2PHR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-link-yECFP-Hygro

   Plasmid

   #44647

   Depositor

   Kurt Thorn

   Insert

   yEmCFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPOM033_P7_3"His5

   Plasmid

   #216458

   Purpose

   3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   3''his5
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPOM026_P2_pNMT1

   Plasmid

   #216451

   Purpose

   Regulatable promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter nmt1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPOM019_P2_pRPL2102

   Plasmid

   #216444

   Purpose

   Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter rpl2102
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPOM015_P2_pADH1

   Plasmid

   #216440

   Purpose

   Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter adh1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPOM013_P2_pENO101

   Plasmid

   #216438

   Purpose

   Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter eno101
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCEV-G2-Km ymScarletI

    Plasmid

    #193960

    Purpose

    TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymScarletI in budding yeast

    Depositor

    Bas Teusink

    Insert

    ymScarletI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pI3-MTU-DO-G418

    Plasmid

    #160023

    Purpose

    Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). G418 selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS406 ATG9 stopGFP

    Plasmid

    #114669

    Purpose

    Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

    Depositor

    Daniel Klionsky

    Article

    Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

    Insert

    ATG9
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Changed the three linker base pairs after the las…

    Promoter

    ATG9

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. MAP72_MS2

    Plasmid

    #220623

    Purpose

    Cassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.

    Depositor

    Marvin Wickens

    Article

    Preston et al Nat Methods. 2019 May;16(5):437-445. doi

    Type

    Empty backbone

    Use

    Shuttle vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-link-yoTagRFP657-Kan

    Plasmid

    #44955

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized TagRFp657 with Kan selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoTagRFP657
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAWH-largeT

    Plasmid

    #133895

    Purpose

    Positive control when used in combination with pBWH-p53. Negative control when used in combination with pBWH-Lam. Expression of Gal4AD-largeT hybrid protein. Homology regions for recombination with pB

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Insert

    LargeT
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pESC-URA-GFP-Ubc9wt

    Plasmid

    #20368

    Depositor

    Judith Frydman

    Article

    Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

    Insert

    SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)
    

    Use

    Yeast/bacteria shuttle

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pYM29-BirA*

    Plasmid

    #160291

    Purpose

    Used for C-terminal BirA* tagging of proteins in Saccharomyces cerevisiae; Selection marker: TRP1

    Depositor

    Martin Ott

    Article

    Salvatori et al Mol Cell. 2020 Feb 20;77(4):887-900.e5.

    Insert

    BirA*
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS316-4M5.3

    Plasmid

    #45831

    Depositor

    Dane Wittrup

    Insert

    4M5.3
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEZY202

    Plasmid

    #18705

    Purpose

    pEG202 converted Gateway Destination vector for yeast two-hybrid bait vector

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Use

    Two-hybrid bait vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIW447-CRISPR XYL2

    Plasmid

    #98906

    Purpose

    K. marxianus CRISPR Plasmid for XYL2 editing

    Depositor

    Ian Wheeldon

    Article

    Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

    Inserts

    Codon optimized Cas9
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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