8,432 results
-
Plasmid#232743PurposeExpresses NADPH/NADP+ biosensor NAPstar4.3 in S. cerevisiae.DepositorInsertNAPstar4.3
ExpressionYeastAvailable sinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymScarletI3-URA3
Plasmid#224075PurposeYeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with URA3 selectionDepositorInsertymScarletI3
TagsymScarletI3ExpressionYeastAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::URA3
Plasmid#227595PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::URA3
Tags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIV270
Plasmid#226181PurposeExpresses Cas9 and gRNA targeting KU70 gene in K. phaffii.DepositorInserttRNA-sgRNA-tRNA
UseCRISPRExpressionBacterial and YeastPromoterTEF1p (Komagataella phaffii)Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-TRP (V5 and HA)
Plasmid#222488PurposeCo-expression of two genes in yeast with V5 and HA tags and TRP1 selectionDepositorTypeEmpty backboneTagsHA and V5ExpressionYeastAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYDS2.0_AT118-H
Plasmid#222846PurposeYeast-display plasmid encoding Nb. AT118-H, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)DepositorInsertAT118-H
ExpressionYeastAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 mBaojin-T2A-mCherry
Plasmid#219842Purposeequimolar expression of mBaojin and mCherry in budding yeastDepositorInsertmBaojin-T2A-mCherry
ExpressionYeastAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAP
Plasmid#206429PurposeA vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pABY-T1
Plasmid#201764PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
ExpressionYeastAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-yomRuby3-SpHIS5
Plasmid#204287PurposeFor C-terminal tagging using yeast optimized mRuby3DepositorInsertpFA6-yomRuby3-SpHIS5
TagsyomRuby3ExpressionYeastPromoternoneAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ2
Plasmid#197415PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-2 σ2
TagsGAL4-DNA binding domain fragment, HA tag, and nuc…ExpressionYeastMutationencodes R42G substitution, contains silent substi…PromoterADH1 and MET25Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGS_128
Plasmid#160651PurposeExpresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoterDepositorInsertApolipoprotein E e3 (APOE Human)
TagsKar2 signal sequenceExpressionYeastMutatione3 allelePromoterpZ estradiol inducible promoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWS3865
Plasmid#185742PurposeSub-array backbone 1/4DepositorTypeEmpty backboneExpressionYeastAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-HALO-NAT
Plasmid#188931PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-HALO-NAT
ExpressionYeastAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-HALO-URA
Plasmid#188932PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-HALO-URA
ExpressionYeastAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV537
Plasmid#177704PurposePlasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hanseniiDepositorInsertsCas9
Nat
tRNA-sgRNA-tRNA
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LT1_34_sfpHluorin
Plasmid#177707PurposePlasmid for genome integration in X2 site expressing sfpHluorin (intracellular pH biosensor)DepositorInsertpTEF1-sfpHluorin
UseUsed as donor in genome integration after lineari…ExpressionYeastAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
LT2_7_OxPro
Plasmid#177709PurposePlasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)DepositorInsertspTRX2_5xUAS-ymYPET-tCYC1
pTEFmut8-mCherry-tADH1
UseUsed as donor dna in genome integration after lin…ExpressionYeastAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-DHFR F[3]-N (LEU2)
Plasmid#177798PurposeGateway destination vector to insert genes of interest having a N-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneExpressionYeastPromoterADH1Available sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
EC2_3_dCas9_Mxi1_sgRNA
Plasmid#163708PurposeYeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressorDepositorInsertsdCas9
Mxi1+SV40 NLS
ExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only