8,350 results
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Plasmid#165968PurposeExpresses rTetTA (K8N, L131L) mutantDepositorInserttetR
UseSynthetic BiologyTagsthree copies of VP16 (VP48)ExpressionYeastAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRG201
Plasmid#64394PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTEF6-7
Plasmid#124583PurposeReporter plasmid with constitutively expressed mCherry and YFP (eCitrine)DepositorInsertmCherry
ExpressionYeastAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
GAL FLAG72QDProGFP p306
Plasmid#15833DepositorInserthtt 72Q Delta Pro (HTT Human)
TagsFLAG and GFPExpressionYeastMutationProline-rich region removedAvailable SinceOct. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCfB8787
Plasmid#126921PurposePlasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of HMG1DepositorInsertHMG1
ExpressionYeastAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fSNAP-Hygromycin
Plasmid#188914PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fSNAP-Hygromycin
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
p403 ā TDH3-MTS-mCherry-GFP1-10-His
Plasmid#177730PurposemCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS markerDepositorInsertmCherry-GFP1-10
TagsMitochondrial Targeting SequenceExpressionBacterial and YeastAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK94 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (KanR)
Plasmid#179078PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYN2_1
Plasmid#184757PurposePlasmid for genome editing by CRISPR/Cas9DepositorInsertsCas9
sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
UseSynthetic BiologyTags2xNLSExpressionBacterial and YeastPromoterPGK1 and tRNA-Phe-HDV RibozymeAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWS3866
Plasmid#185743PurposeSub-array backbone 2/4DepositorTypeEmpty backboneExpressionYeastAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV0526
Plasmid#133500PurposeExpression of sfGFP targeted to the nucleus with one NLS signalsDepositorInsertpHis5(StuI)-p(tdh1)-sfGFP-NLSSV40-natMX
ExpressionYeastAvailable SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB4586
Plasmid#106124PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
YEp181-CUP1-His-Smt3
Plasmid#99536PurposeYeast episomal vector for expression of His-tagged Smt3 (SUMO); LEU2 markerDepositorAvailable SinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIMVAu1
Plasmid#98301PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.DepositorInsertHMG2(-309,-153)-HIS3-TEFM1ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)
ExpressionYeastAvailable SinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
PRS415-Gal1pr-sctetR-FokI
Plasmid#44756DepositorAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
UASGAL-Pgal
Plasmid#60338PurposeContains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoterDepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promoteā¦ExpressionYeastAvailable SinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBBK03 pCAS-Tyr-[gRNA: BsaI GFP dropout] (HygR,AmpR)
Plasmid#178987PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDBTrp-LexABD-CRY2FL
Plasmid#78210PurposeUse with Addgene 40615 for blue light transcription control in yeastDepositorInsertLexABD-CRY2 (CRY2 Mustard Weed)
TagslexA binding domainExpressionYeastMutationcontains endogenous CRY2 NLSPromoterADH1Available SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
ZJOM10
Plasmid#133639PurposeIntegration of VPS4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast late endosome.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHES795
Plasmid#87943PurposeConstitutively expresses estradiol-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.DepositorInsertZif268 DBD - hER LBD - MSN2 AD (ESR1 Budding Yeast, Synthetic, Human)
ExpressionYeastPromoterADH1 (crippled)Available SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only