8,223 results
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Plasmid#104342PurposeExpress yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertpTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
Tags3xFLAGExpressionYeastAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV10.KH
Plasmid#63208PurposepCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-G9-5FLAG-hphMX6
Plasmid#49185Purposegenomic G9-5FLAG epitope tagging in S. pombeDepositorTypeEmpty backboneTagsG9-5FLAGExpressionYeastAvailable SinceNov. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pE3n
Plasmid#17457Purposemodified pENTR vector for N-terminal 6x MYC tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTags6xMycExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDB4280
Plasmid#98699PurposeA Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 systemDepositorInsertCas9
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ymTurquoise2-CaURA3
Plasmid#118458PurposeYeast tagging vector to create a gene fusion with yeast optimized mTq2 with CaUra3 selection. *An updated version of this plasmid is available: #168057 pFA6a-link-ymTq2-URA3.DepositorInsertymTurquoise2
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB5791
Plasmid#106126PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntF_2, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGAL-PDE1
Plasmid#160388PurposeOverxpression of PDE1 in yeastDepositorInsertPDE1
ExpressionYeastPromoterGALAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-Z3-Nat
Plasmid#232100PurposeYeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.DepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-Z3-Hyg
Plasmid#232095PurposeYeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertZ3 promoter and Z3EV transcription factor
UseCRISPRExpressionYeastPromoterZ3Available SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Nat-ADE2
Plasmid#232103PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWS625
Plasmid#123046PurposeAdenosine GPCRDepositorInsertA2BR [3]
ExpressionYeastAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pTEF1p-CYC1t
Plasmid#62190PurposeExpresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionYeastMutationhuman codon-optimized, D10A, H840APromoterTEF1 promoterAvailable SinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yECitrine-Hygro
Plasmid#44646DepositorInsertyECitrine
ExpressionYeastAvailable SinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
PP244
Plasmid#78978PurposePromoter: ppTEF1, minimal Promoter: GAP. Figure 4. Expresses GFP.DepositorInsertGFP
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIM573
Plasmid#74637Purposealias pFA6a-ymTag-BFP-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and G418 selection markerDepositorInsertymTagBFP
ExpressionYeastPromoternoneAvailable SinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-Tom7
Plasmid#66571PurposeConstitutively express mitochondrial localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsTom7 and mCherryExpressionYeastMutationPhyB portion contains aa's 1-908PromoterpADH1Available SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB769
Plasmid#60138PurposeRed click beetle luciferase from Promega driven by MET17 promoter.DepositorInsertCBR
ExpressionYeastPromoterMET17prAvailable SinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMT1420
Plasmid#228316PurposeHomoserine lactone (HSL)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoterHomoserine lactone (HSL)-regulatable synthetic pr…Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT950
Plasmid#228290Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only