8,086 results

1. pDRF1-GW AKAR3-EV

   Plasmid

   #182533

   Purpose

   Expresses the PKA FRET sensor AKAR3-EV in budding yeast

   Depositor

   Bas Teusink

   Article

   Botman et al FEMS Yeast Res. 2023 Jan 4;23:foad029. d

   Insert

   AKAR3-EV
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PMA1

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS415_pGal-nCas9(H840A)

   Plasmid

   #79616

   Purpose

   Expresses nCas9(H840A) in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H840A for nickase Cas9

   Promoter

   pGal1

   Available Since

   Sept. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMJA180

   Plasmid

   #128538

   Purpose

   Expresses E coli Bir A protein

   Depositor

   Marcos Alcocer

   Article

   de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

   Insert

   E coli BirA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available Since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pI1-MTU-DO-URA

   Plasmid

   #160009

   Purpose

   Integrative vector for K. marxianus, targeting the LAC4 locus. Uracil selection.

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBBK80 pHomL-RPL18Bp-Scarlet(rfp)-Link-VAM3-SSA1t-HomR (AmpR)

   Plasmid

   #179064

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VAM3, a marker for the vacuole

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-Scarlet(rfp)-Link-VAM3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTCW012

   Plasmid

   #83527

   Purpose

   Cloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminator

   Depositor

   Claudia Vickers

   Article

   Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pFUS1J2

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAV0785

   Plasmid

   #133507

   Purpose

   Expression of mCherry tagged PCNA to monitor DNA replication

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pUra4(AfeI)-p(pcn1)-mCherry-pcn1-terminator(nmt1)-natMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMEL16

   Plasmid

   #107922

   Purpose

   HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBW-m4046663

   Plasmid

   #153857

   Purpose

   Yeast expression plasmid for SARS-CoV-2 nsp3

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pCUPI NPD-GFP

    Plasmid

    #1191

    Depositor

    Susan Lindquist

    Article

    Patino et al Science 1996 Aug 2;273(5275):622-6.

    Insert

    N-terminus of Sup35 (SUP35 Budding Yeast)
    

    Use

    Cup1 promoter

    Tags

    GFP

    Expression

    Yeast

    Mutation

    fused N-terminal Sup35 (~1-253aa) to GFP

    Promoter

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pKT0210

    Plasmid

    #8724

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    yECFP A206R
    

    Use

    Tags

    Codon optimized linker

    Expression

    Yeast

    Mutation

    yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A,…

    Promoter

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. YIPlac204TKC-GFP-HDEL

    Plasmid

    #21771

    Depositor

    Benjamin Glick

    Article

    Rossanese et al J Cell Biol. 2001 Apr 2. 153(1):47-62.

    Insert

    Green Fluorescent Protein (GFP)
    

    Use

    Tags

    HDEL and preKar2

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWS176

    Plasmid

    #90963

    Purpose

    Cas9 gap repair vector - ZeoR

    Depositor

    Tom Ellis

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YIp128-P30-His-POL30

    Plasmid

    #99542

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA); LEU2 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    POL30

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPICZc-HsACTG1*-thymosinB-8His

    Plasmid

    #111147

    Purpose

    Expresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.

    Depositor

    Mohan Balasubramanian

    Article

    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

    Insert

    actin gamma 1 (ACTG1 Human)
    

    Use

    Pichia pastoris integration vector

    Tags

    Thymosin beta and a His-tag

    Expression

    Yeast

    Mutation

    codon-optimised for expression in Pichia pastoris

    Promoter

    AOX1

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSC5GGv2

    Plasmid

    #188604

    Purpose

    pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS426-GFP-Osh2-PH

    Plasmid

    #71373

    Purpose

    For analysis of PH domain localization in yeast

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Osh2-PH
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Pleckstrin homology (PH) domain; aa 277-398

    Promoter

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pYX113-mtGFP

    Plasmid

    #45049

    Depositor

    Benedikt Westermann

    Article

    Westermann et al Yeast. 2000 Nov;16(15):1421-7.

    Insert

    mtGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available Since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pAG423GPD-ccdB-EGFP

    Plasmid

    #14198

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pACT2.2gtwy

    Plasmid

    #11346

    Depositor

    Guy Caldwell

    Insert

    gateway cassette
    

    Use

    Tags

    Gal4 Activation Domain

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only