7,998 results

1. pEasyG1_hph

   Plasmid

   #184912

   Purpose

   Template to generate via PCR a single gRNA for expression in S. cerevisiae

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pI6-MTU-DO-URA

   Plasmid

   #160030

   Purpose

   Integrative vector for K. marxianus, targeting the ARO3 locus. Uracil selection. Low integration efficiency in an NHEJ-deficient background.

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pI2-MTU-DO-G418

   Plasmid

   #160018

   Purpose

   Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). G418 selection.

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB3052(gRNA X-4, XI-3, XII-5)

   Plasmid

   #73294

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG425GPD-EGFP-ccdB

   Plasmid

   #14322

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACT2.2gtwy

   Plasmid

   #11346

   Depositor

   Guy Caldwell

   Insert

   gateway cassette
   

   Use

   Tags

   Gal4 Activation Domain

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pFA6a-link-yoLSS-mKate2-Kan

   Plasmid

   #44943

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoLSS-mKate2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. p413TEF-Sir2-H364Y

   Plasmid

   #51743

   Purpose

   overexpression of Sir2 in S. cerevisiae (H364Y mutant), HIS3 marker

   Depositor

   Markus Ralser

   Article

   Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

   Insert

   Sir2 (SIR2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H364Y

   Promoter

   TEF1

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. dCas9v2

   Plasmid

   #103140

   Purpose

   TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site

   Depositor

   Jens Nielsen

   Article

   Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

   Insert

   dCas9-VPR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Removed scaffold gRNA from original plasmid

   Promoter

   Available since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-6xGLY-T7-kanMX6

    Plasmid

    #20760

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYM28-BirA*

    Plasmid

    #160290

    Purpose

    Used for C-terminal BirA* tagging of proteins in Saccharomyces cerevisiae; Selection marker: HIS3

    Depositor

    Martin Ott

    Article

    Salvatori et al Mol Cell. 2020 Feb 20;77(4):887-900.e5.

    Insert

    BirA*
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFS181

    Plasmid

    #12536

    Depositor

    Nick Rhind

    Article

    Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

    Insert

    human equilibrating nucleoside transporter 1 (SLC29A1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2B-57

    Plasmid

    #177927

    Purpose

    Expresses yeGFP under control of doxycycline-responsive TET3 promoter (contains three rtTA binding sites)

    Depositor

    Vadim Gladyshev

    Article

    Barre et al (unpublished)

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TET3 (contains three rtTA binding sites)

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. ZJOM156

    Plasmid

    #133636

    Purpose

    Integration of 2xGFP tag at SEC7 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    SEC7-2GFP (SEC7 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJW1663

    Plasmid

    #112037

    Purpose

    Gal-Estradiol transcription activation expression cassette marked with KanR

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    Gal DNA binding-estradiol binding-MSN2 activator
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRG216

    Plasmid

    #64528

    Purpose

    Centromeric E. coli/S. cerevisiae shuttle vector

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDS2149

    Plasmid

    #184275

    Purpose

    Tet-on genome insertion system

    Depositor

    Dominique Sanglard

    Insert

    CatrTA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pND003

    Plasmid

    #200949

    Purpose

    Yeast surface display destination vector for Golden Gate cloning

    Depositor

    Timothy Whitehead

    Article

    Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

    Type

    Empty backbone

    Use

    Yeast surface display

    Tags

    AGA2, HA, and Myc

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYES2-Ub-R-GFP

    Plasmid

    #11953

    Depositor

    Nico Dantuma

    Article

    Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

    Insert

    Ub-R-GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Ubiquitin fused to N-terminus of GFP. Arginine at…

    Promoter

    Available since

    May 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS-SKII-3XHA-fSNAP-URA

    Plasmid

    #188917

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-fSNAP-URA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge