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pNK5785

Plasmid

#219753

Purpose

pGAP-like vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - nnLuz_v4 - tAOX

Use

Luciferase

Expression

Yeast

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-Alpha-synuclein

Plasmid

#181715

Purpose

Galactose inducible expression of Alpha-synuclein

Depositor

Alejandro Chavez

Insert

Alpha-synuclein (SNCA Human)

Expression

Yeast

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pEK36

Plasmid

#210598

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

SpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter

Tags

mClover3

Expression

Bacterial and Yeast

Promoter

SpH2Bpro

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSAP

Plasmid

#206429

Purpose

A vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).

Depositor

Bogumil Karas

Article

Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pYZ960

Plasmid

#187972

Purpose

TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2µ-URA3

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Promoter

TEF1

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ45: pRS426-His-MBP-SpuFz1_Native

Plasmid

#205252

Purpose

S. cerevisiae expression vector for SpuFz1 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

10xHis, MBP

Expression

Yeast

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDB5322

Plasmid

#204832

Purpose

An integrating plasmid containing both the inducible enotetSW4 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

Depositor

Li-Lin Du

Article

Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

Inserts

tetR
mECitrine

Expression

Yeast

Promoter

CMV promoter and enotetSW4 promoter

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pYZ462

Plasmid

#187970

Purpose

TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pABY-T1

Plasmid

#201764

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Expression

Yeast

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ106: pRS426-His-MBP-NlovFz2_Native

Plasmid

#205253

Purpose

S. cerevisiae expression vector for NlovFz2 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2

Tags

10xHis, MBP

Expression

Yeast

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSc-humanADAR2

Plasmid

#171106

Purpose

Expresses human ADAR2 in yeast.

Depositor

Brenda Bass

Article

Macbeth et al Methods Enzymol. 2007;424:319-31. doi: 1

Insert

human ADAR2 (ADARB1 Human)

Tags

His12_TEV

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pRS403-HIS3-pINO4-SV40NLS-PfV-GS-Sapphire

Plasmid

#203658

Purpose

Yeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm GEMs under the HIS3 promoter.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Tags

Sapphire

Expression

Yeast

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRS305-LEU2-pINO4-SV40NLS-PfV-GS-Sapphire

Plasmid

#203655

Purpose

Yeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Tags

Sapphire

Expression

Yeast

Available Since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-yomRuby3-SpHIS5

Plasmid

#204287

Purpose

For C-terminal tagging using yeast optimized mRuby3

Depositor

Linda Huang

Article

Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

Insert

pFA6-yomRuby3-SpHIS5

Tags

yomRuby3

Expression

Yeast

Promoter

none

Available Since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCast

Plasmid

#200169

Purpose

E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

Depositor

Yong-Su Jin

Article

Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pWL078 MCP-2xGFP

Plasmid

#197047

Purpose

pCYC-NLS-MCP-2xGFP on pRS305

Depositor

Robert Singer

Article

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

MCP-2xGFP

Expression

Yeast

Promoter

CYC1

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEasyCas9

Plasmid

#184909

Purpose

Expresses the Streptococcus pyogenes Cas9 protein

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

Cas9

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pGS_107

Plasmid

#160650

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

GAL1

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pGS_129

Plasmid

#160652

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

pZ estradiol inducible promoter

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pWS3865

Plasmid

#185742

Purpose

Sub-array backbone 1/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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