8,086 results

1. pFA6a-link-yomKeima-Kan

   Plasmid

   #44902

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mKeima with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomKeima
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. S3_001_026_MV2

   Plasmid

   #134422

   Purpose

   pYTKMV2-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

   Depositor

   Mattheos Koffas

   Article

   Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

   Inserts

   mCherry
   NLS_FdeR-N
   

   Use

   Tags

   none

   Expression

   Yeast

   Mutation

   none

   Promoter

   pGMP1 and pTDH3

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-6xGLY-T7-hphMX4

   Plasmid

   #20761

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. M3925 trp1::KanMX3 Disruptor Converter

   Plasmid

   #51681

   Purpose

   Yeast marker swap plasmid for converting Trp1 to KanMX3

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   KanMX3
   

   Use

   Yeast marker swap

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUG-amdSYM-mak10TR

   Plasmid

   #75433

   Purpose

   Generates truncated version of MAK10 gene, which is required for dsRNA L-A propagation in S.cerevisiae

   Depositor

   John McCusker

   Inserts

   MAK10TR
   ApR
   amdSYM
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   N-terminal 740 bp of MAK10 ORF

   Promoter

   Native promoter and PTEF

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS406-CMV-lacI-3FLAG

   Plasmid

   #83410

   Purpose

   Expression of lacI in yeast

   Depositor

   Toshi Tsukiyama

   Article

   Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

   Insert

   lacI repressor
   

   Use

   Tags

   3xFLAG and NLS

   Expression

   Yeast

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBW-m4046647

   Plasmid

   #153940

   Purpose

   Yeast expression plasmid for SARS-CoV-2 3C-like proteinase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAG304GAL-ccdB

   Plasmid

   #14135

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. JME4390

   Plasmid

   #129656

   Purpose

   LEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYES2-Ub-R-GFP

    Plasmid

    #11953

    Depositor

    Nico Dantuma

    Article

    Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

    Insert

    Ub-R-GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Ubiquitin fused to N-terminus of GFP. Arginine at…

    Promoter

    Available Since

    May 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB2193

    Plasmid

    #67542

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG426GAL-ccdB-DsRed

    Plasmid

    #14371

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    DsRed

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHS12-mCherry

    Plasmid

    #25444

    Depositor

    Benjamin Glick

    Insert

    mCherry
    

    Use

    Tags

    COX4 Presequence

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCfB3052(gRNA X-4, XI-3, XII-5)

    Plasmid

    #73294

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

    Plasmid

    #58439

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEV-G2-Ph

    Plasmid

    #46816

    Purpose

    TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

    Depositor

    Claudia Vickers, Lars Nielsen

    Article

    Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

    Type

    Empty backbone

    Use

    Tags

    FLAG and c-myc

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 and PGK1

    Available Since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. Osa-pCAMBIA-1301-STTM319

    Plasmid

    #84201

    Purpose

    Disturb the expression of miR319 in rice

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR319
    

    Use

    Tags

    Expression

    Bacterial, Plant, and Yeast…

    Mutation

    Promoter

    2x35S

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFA6a-mTID-HA3-KanMX6

    Plasmid

    #227982

    Purpose

    To insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    miniTurboID
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCfB3045(gRNA XI-3)

    Plasmid

    #73287

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-3

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. YIp TRP1-HphMx

    Plasmid

    #122922

    Purpose

    TRP1-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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