8,057 results

1. LPH2781 - Ub-K63R

   Plasmid

   #32160

   Depositor

   Linda Hicke

   Insert

   synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K63R

   Promoter

   CUP1

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pFA6a-natMX6-P41nmt1-3FLAG

   Plasmid

   #19346

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

   Insert

   natMX6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. KBB364

   Plasmid

   #185083

   Purpose

   RFA1 fused to GFP under control of GAL1 promoter

   Depositor

   Ken Belanger

   Article

   Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

   Insert

   RFA1
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAct::Raf

   Plasmid

   #11341

   Depositor

   Michael Wigler

   Article

   Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90

   Insert

   byr-2 (byr2 Fission Yeast)
   

   Use

   Tags

   Gal4 activation domain

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

5. ZJOM156

   Plasmid

   #133636

   Purpose

   Integration of 2xGFP tag at SEC7 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   SEC7-2GFP (SEC7 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. p426 46Q GPD

   Plasmid

   #1182

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 46Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

7. p2B-57

   Plasmid

   #177927

   Purpose

   Expresses yeGFP under control of doxycycline-responsive TET3 promoter (contains three rtTA binding sites)

   Depositor

   Vadim Gladyshev

   Article

   Barre et al (unpublished)

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TET3 (contains three rtTA binding sites)

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJW1666

   Plasmid

   #112040

   Purpose

   CEN/ARS plasmid for GAL-inducible empty reporter V5-TEV-EGFP tagged expression marked with URA

   Depositor

   Jonathan Weissman

   Article

   Costa et al Science. 2018 Jan 18. pii: science.aar36

   Insert

   None
   

   Use

   Tags

   V5-TEV-EGFP

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGI3EM09

   Plasmid

   #135482

   Purpose

   Agrobacterium T-DNA with SpunHsp70pr driving hph-GFP

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   SpunHsp70pr hph-GFP ADH1t
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpunHsp70pr

   Available since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson

    Plasmid

    #117662

    Purpose

    Test intracellular localisation of E2-Crimson and study the secretion efficiency

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pOst1-pro-af(MUT1)-E2-Crimson
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    This plasmid encodes the fluorescent protein E2-C…

    Promoter

    pAOX1

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIMVAd40R

    Plasmid

    #98309

    Purpose

    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of GAL promoters; integrated into ERG9 locus;

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

    Insert

    ERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAG416GPD-ccdB-DsRed

    Plasmid

    #14364

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    DsRed

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB2795

    Plasmid

    #63650

    Purpose

    pCfB2795 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Insert

    GFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. HSP104-b/Leu(WT)

    Plasmid

    #1156

    Depositor

    Susan Lindquist

    Article

    Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

    Insert

    HSP104 (HSP104 Budding Yeast)
    

    Use

    Tags

    GAL1:10 promoter

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. YIplac211-iGFP-TLG1

    Plasmid

    #105261

    Purpose

    Plasmid for pop-in/pop-out replacement of Tlg1 with iGFP-Tlg1.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Tlg1 (TLG1 Budding Yeast)
    

    Use

    Tags

    iGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. yHS1

    Plasmid

    #159150

    Purpose

    Cytosolic labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    yHS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pI6-MTU-DO-URA

    Plasmid

    #160030

    Purpose

    Integrative vector for K. marxianus, targeting the ARO3 locus. Uracil selection. Low integration efficiency in an NHEJ-deficient background.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAH237

    Plasmid

    #121146

    Purpose

    Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast

    Depositor

    Katsunori Tanaka

    Article

    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

    Inserts

    gRNA cassette
    humanized Streptococcus pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Flag

    Expression

    Yeast

    Mutation

    Promoter

    S.pombe rrk1 and nmt41

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEasyG1_hph

    Plasmid

    #184912

    Purpose

    Template to generate via PCR a single gRNA for expression in S. cerevisiae

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDRF1 mScarlet1.4-T2A-mTurquoise2

    Plasmid

    #219849

    Purpose

    equimolar expression of mScarlet1.4 and mTurquoise2 in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2025.06.27.661975; doi: https://

    Insert

    mScarlet1.4-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge