8,057 results

1. pND003

   Plasmid

   #200949

   Purpose

   Yeast surface display destination vector for Golden Gate cloning

   Depositor

   Timothy Whitehead

   Article

   Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

   Type

   Empty backbone

   Use

   Yeast surface display

   Tags

   AGA2, HA, and Myc

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB2792

   Plasmid

   #63653

   Purpose

   pCfB2792 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Insert

   GFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV0751

   Plasmid

   #133485

   Purpose

   Expression in fission yeast from promoter pnmt1 , integrated at ura4 locus

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pUra4(AfeI)-p(nmt1)-sfGFP-terminator(ScCYC1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAGE2.0-i

   Plasmid

   #194639

   Purpose

   Empty pAGE2.0 plasmid with pBAD inducible promoter

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

   Type

   Empty backbone

   Use

   Synthetic Biology; Diatom expression

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPICZ-pre-pro-alphaf-E2-Crimson

   Plasmid

   #117658

   Purpose

   Test intracellular localisation of E2-Crimson and study the secretion efficiency

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pre-pro-alphaf-E2-Crimson
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   This plasmid encodes the fluorescent protein E2-C…

   Promoter

   pAOX1

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-natMX6-P3nmt1-3FLAG

   Plasmid

   #19345

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

   Insert

   natMX6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG426GPD-EGFP-ccdB

   Plasmid

   #14324

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDS2149

   Plasmid

   #184275

   Purpose

   Tet-on genome insertion system

   Depositor

   Dominique Sanglard

   Insert

   CatrTA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBEVY-4420-mCherry

   Plasmid

   #218828

   Purpose

   Yeast surface display of an anti-fluorescene scFv and intracellular expression of mCherry

   Depositor

   Yongku Cho

   Article

   Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

   Insert

   4-4-20 scFv
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBEVY-pT231 scFv-GFP

    Plasmid

    #218827

    Purpose

    Yeast surface display of a phospho-tau pT231 scFv and intracellular expression of GFP

    Depositor

    Yongku Cho

    Article

    Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

    Insert

    pT231 tau scFv
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pE3c

    Plasmid

    #17463

    Purpose

    modified pENTR vector for C-terminal 6x MYC tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    6xMyc

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBW-m4046233

    Plasmid

    #149032

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pYES2.1-pomMet26

    Plasmid

    #107448

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    Met26 (met26 Fission Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYES2.1-pomMet16

    Plasmid

    #107452

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    Met16 (met16 Fission Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPL5628_TEF1*-Cre_MET15

    Plasmid

    #60933

    Purpose

    Constitutive expression vector for Cre-recombinase containing MET15 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Use

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Mutation

    Promoter

    mutant TEF1

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. YIplac211-VPS8-HaloTagx2

    Plasmid

    #177113

    Purpose

    Expresses Vps8-HaloTagx2 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    VPS8 (VPS8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAV0328

    Plasmid

    #133489

    Purpose

    Expression of nucleo-cytoplasmic mCherry

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(act1)-mCherry-terminator(tdh1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDRF1 ymStayGold-T2A-mCherry

    Plasmid

    #219843

    Purpose

    equimolar expression of ymStayGold and mCherry in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2025.06.27.661975; doi: https://

    Insert

    ymStayGold-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMEL10

    Plasmid

    #107916

    Purpose

    URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS-SKII-3XHA-HALO-Kanamycin

    Plasmid

    #188933

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-HALO-Kanamycin
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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