8,086 results

1. LHP398 - Ub-K48R/K63R

   Plasmid

   #32167

   Depositor

   Linda Hicke

   Insert

   synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K48R and K63R

   Promoter

   CUP1

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pAV115

   Plasmid

   #63182

   Purpose

   pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG425GAL-ccdB-HA

   Plasmid

   #14249

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIM700

   Plasmid

   #74639

   Purpose

   alias pFA6a-yTagRFP-T-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and nourseothricin selection marker

   Depositor

   Jiří Hašek

   Article

   Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

   Insert

   yTagRFP-T
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available Since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. M3938 trp1::ADE2 Disruptor Converter

   Plasmid

   #51673

   Purpose

   Yeast marker swap plasmid for converting TRP1 to ADE2

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   ADE2 (ADE2 Budding Yeast)
   

   Use

   Yeast marker swap

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADE2

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-link-yoGFPgamma-SpHis5

   Plasmid

   #44859

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized GFPgamma with SpHis5 selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoGFPgamma
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. YIp ADE2-LEU2

   Plasmid

   #122905

   Purpose

   ADE2-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICXC3GH

   Plasmid

   #49023

   Purpose

   FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site), GFP, and dec…

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. p41Hyg 1-F GW

   Plasmid

   #58547

   Purpose

   CEN vector with Hygromycin resistance marker for single Gateway insertion

   Depositor

   Leonid Kruglyak

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pESC-URA-Rnq1-GFP

    Plasmid

    #21051

    Depositor

    Judith Frydman

    Article

    Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

    Insert

    Rnq1 (RNQ1 Budding Yeast)
    

    Use

    Yeast/bacteria shuttle

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pNB648

    Plasmid

    #60140

    Purpose

    NanoLuc luciferase with a nuclear localization signal (NLS) driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    NLuc
    

    Use

    Tags

    Nuclear localization signal (NLS)

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pREF-GFP kan

    Plasmid

    #122061

    Purpose

    reporter plasmid for termination and terminator override to express GFP. Marked with URA3

    Depositor

    Daniel Reines

    Article

    Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

    Inserts

    intergenic terminator from yeast IMD2
    green fluorescent protein
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL1 comes in pYES2

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. LD209

    Plasmid

    #69182

    Purpose

    Integration of 2GFP tag at Atg9 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg9-2EGFP
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG416GAL-EMCV_3C

    Plasmid

    #201941

    Purpose

    Expresses EMCV 3C protease from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    EMCV 3C protease (EMCVgp1 )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCfB9336 (pgRNA_II-1_NatMX)

    Plasmid

    #161589

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site II-1

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Insert

    guiding RNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJOG417

    Plasmid

    #105341

    Purpose

    Yeast two hybrid

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJOG418

    Plasmid

    #105342

    Purpose

    Yeast two hybrid

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006)

    Plasmid

    #173453

    Purpose

    Contains mCherry-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPTK020-7-attB

    Plasmid

    #84989

    Purpose

    Type 7, BxbI recognition site

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    BxbI recognition site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    BsaI site removed (1711 t to a)

    Promoter

    Non

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJT82_GalL_nCDA1Δ198-BE3

    Plasmid

    #145046

    Purpose

    Expresses nCDA1Δ198-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1Δ198-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    pmCDA1(1-198AA); spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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