8,058 results

1. ZJOM103

   Plasmid

   #133664

   Purpose

   Integration of SEC7-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-SEC7-mTagBFP2 (SEC7 Budding Yeast)
   

   Use

   Tags

   mTagBFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. LHP1463 - INP52

   Plasmid

   #32432

   Depositor

   Linda Hicke

   Article

   Stamenova et al J Biol Chem. 2004 Apr 16;279(16):16017-2

   Insert

   INP52 (INP52 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Lac

   Available since

   Nov. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pPTK002-2-pGAP

   Plasmid

   #84961

   Purpose

   Type 2, pGAP, Glyceraldehyde-3-phosphate dehydrogenase promoter

   Depositor

   Volker Sieber

   Article

   Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

   Insert

   Glyceraldehyde-3-phosphate dehydrogenase promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   BsaI site removed (1805 c to g)

   Promoter

   Non

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYes2-TaPCS1

   Plasmid

   #49767

   Purpose

   Expresses wheat phytochelatin synthase (TaPCS1) in Saccharomyces Cerevisiae

   Depositor

   Julian Schroeder

   Article

   Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

   Insert

   TaPCS1 (PCS1 Triticum aestivum)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pHES822

   Plasmid

   #89193

   Purpose

   Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   pZ-YFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pZ

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYES-mtBFP

   Plasmid

   #45055

   Depositor

   Benedikt Westermann

   Article

   Westermann et al Yeast. 2000 Nov;16(15):1421-7.

   Insert

   mtBFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL

   Available since

   May 24, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pYX223-mtGFP

   Plasmid

   #45051

   Depositor

   Benedikt Westermann

   Article

   Westermann et al Yeast. 2000 Nov;16(15):1421-7.

   Insert

   mtGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL

   Available since

   May 24, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pCfB2337

   Plasmid

   #67555

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pgRNA-backbone

   Plasmid

   #172517

   Purpose

   Yeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertion

   Depositor

   Leonid Kruglyak

   Article

   Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

   Insert

   gRNA cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYB-Dual(mNG(yeast-opt))

    Plasmid

    #216727

    Purpose

    Dual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.

    Depositor

    Emil Jensen

    Article

    Waneskog et al Biotechnol Prog. 2024 May 17:e3482. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    pGAL1(yeast) and pT7-RNAP(bacteria)

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGALSc104(WT)

    Plasmid

    #1146

    Depositor

    Susan Lindquist

    Article

    Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

    Insert

    HSP104 (HSP104 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. pI2-MTU-DO-URA

    Plasmid

    #160015

    Purpose

    Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Uracil selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pI1-MTU-DO-LEU

    Plasmid

    #160013

    Purpose

    Integrative vector for K. marxianus, targeting the LAC4 locus. Leucine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPM56

    Plasmid

    #22855

    Depositor

    Feroz Papa

    Article

    Merksamer et al Cell. 2008 Nov 28. 135(5):933-47.

    Insert

    eroGFP & UPR-RFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pGWKS5

    Plasmid

    #139412

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Citrine marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    Citrine
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTCW007

    Plasmid

    #83525

    Purpose

    pheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene and FUSJ2 promoter

    Depositor

    Claudia Vickers

    Article

    Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

    Insert

    pFUS1J2-mfalpha1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pFUS1J2

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL

    Plasmid

    #79614

    Purpose

    Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Inserts

    SpCas9
    PmCDA1
    

    Use

    Tags

    SH3 domain and SHL

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1 and pGal10

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG303GAL-ccdB-HA

    Plasmid

    #14229

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pV1010

    Plasmid

    #111423

    Purpose

    gRNA plasmid for cleavage of Candida albicans ADE2 - integrates at RPS1 (Part of Duet system)

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    sgADE-NT2
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMC-XI2

    Plasmid

    #176156

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge