8,086 results

1. pKar2-msGFP-HDEL

   Plasmid

   #117668

   Purpose

   Localisation of Endoplasmic Reticulum

   Depositor

   Benjamin Glick, Pau Ferrer

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pKar2-msGFP-HDEL
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Contains the Kar2 promoter and signal sequence fr…

   Promoter

   pKar2

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSM1960

   Plasmid

   #41842

   Depositor

   Susan Michaelis

   Article

   Metzger et al J Biol Chem. 2008 Nov 21;283(47):32302-1

   Insert

   SEC63-mRFP
   

   Use

   Tags

   mRFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV0478

   Plasmid

   #133498

   Purpose

   Expression of sfGFP targeted to the nucleus with two NLS signals

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pHis5(StuI)-p(tdh1)-NLSSV40-sfGFP-NLSSV40-natMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJL-mGold

   Plasmid

   #157997

   Purpose

   Expression of mGold in yeast cells

   Depositor

   Francois St-Pierre

   Article

   Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

   Insert

   mGold
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   mGold is mVenus with L46F;T63S mutations

   Promoter

   pTDH(GAP promoter)

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTL-DHDDS

   Plasmid

   #203177

   Purpose

   Centromeric yeast expression vector for hDHDDS

   Depositor

   William Sessa

   Article

   Galosi et al Brain. 2022 Mar 29;145(1):208-223. doi:

   Insert

   DHDDS (DHDDS Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   RER2

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Yiplac211 proGAL1-SZ1-RGG-GFP-RGG-RGG

   Plasmid

   #176396

   Purpose

   Expresses GFP tagged triple RGG scaffold with N-terminal SynZip1 coiled coil (Sz1). Expression induced in media containing galactose.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   GAL1 promoter, SZ1-RGG-GFP-RGG-RGG
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYES-mtBFP

   Plasmid

   #45055

   Depositor

   Benedikt Westermann

   Article

   Westermann et al Yeast. 2000 Nov;16(15):1421-7.

   Insert

   mtBFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL

   Available Since

   May 24, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pUC19-URA3-3'SEC13-GFP

   Plasmid

   #25445

   Depositor

   Benjamin Glick

   Article

   Rossanese et al J Cell Biol. 1999 Apr 5. 145(1):69-81.

   Insert

   Sec13-Green Fluorescent Protein
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pG23A

   Plasmid

   #102884

   Purpose

   Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Insert

   Cdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
   

   Use

   Tags

   AID(71-114) and mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Native promoter

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB4666

    Plasmid

    #106144

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPM56

    Plasmid

    #22855

    Depositor

    Feroz Papa

    Article

    Merksamer et al Cell. 2008 Nov 28. 135(5):933-47.

    Insert

    eroGFP & UPR-RFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL

    Plasmid

    #79614

    Purpose

    Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Inserts

    SpCas9
    PmCDA1
    

    Use

    Tags

    SH3 domain and SHL

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1 and pGal10

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR)

    Plasmid

    #178989

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMAL498

    Plasmid

    #169154

    Purpose

    Encodes OptoQ-INVRT4

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. 5538 pUG2-SSA1

    Plasmid

    #1247

    Depositor

    Susan Lindquist

    Article

    Glover et al Cell 1998 Jul 10;94(1):73-82.

    Insert

    Ssa1 (SSA1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB2337

    Plasmid

    #67555

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. ZJOM100

    Plasmid

    #133662

    Purpose

    Integration of MNN9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-MNN9-mTagBFP2 (MNN9 Budding Yeast)
    

    Use

    Tags

    mTagBFP2

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHflu2

    Plasmid

    #203880

    Purpose

    pHI1 with insertion of ampicillin-resistance gene

    Depositor

    Bogumil Karas

    Insert

    broad-spectrum class A beta-lactamase TEM-116
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Yeast_Target-AID3S

    Plasmid

    #188646

    Purpose

    Expresses Target-AID3S in yeasts

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Insert

    nCas9-AID2S-UGI
    

    Use

    Tags

    UGI

    Expression

    Yeast

    Mutation

    D10A for SpCas9

    Promoter

    pGal1

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSCL.028

    Plasmid

    #184965

    Purpose

    Test effect of extending -Eco1 a1/a2 in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RT and ncRNA(extended), a1/a2 length: 27
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    human codon optimized RT, a1/a2 length extended t…

    Promoter

    Gal7

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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