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pGR618

Plasmid

#213786

Purpose

Integrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette

Depositor

Chang Liu

Insert

BB-Tv TP-DNAP1

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pRS712

Plasmid

#213041

Purpose

CRISPR activation based overexpression in Nakaseomyces (Candida) glabrata

Depositor

Rebecca Shapiro

Publication

Maroc et al mSphere. 2024 Feb 28;9(2):e0076123. doi:

Type

Empty backbone

Expression

Yeast

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pEK39

Plasmid

#210599

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Publication

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

SpH2Bpro-Hshph-G4S-HsmRuby3-ScADH1ter

Tags

mRuby3

Expression

Bacterial and Yeast

Promoter

SpH2Bpro

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pINTO_7/8

Plasmid

#206431

Purpose

A vector backbone (pCC1BAC-based) containing a single SapI recognition site. When digested, it exposes overhangs that allow integration of the vector into the P. tricornutum chloroplast genome.

Depositor

Bogumil Karas

Publication

Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRS405-FlagPEN2/PS1wt

Plasmid

#207803

Purpose

Yeast integrative vector for expression of FlagPEN2 and Presenilin 1

Depositor

Dieter Langosch

Publication

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Gamma-secretase subunits PEN-2 and presenilin1 wt

Expression

Yeast

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pRS402-nicastrin/APH-1a-HA

Plasmid

#207804

Purpose

Yeast integrative vector for expression of Nicastrin and APH-1a-HA

Depositor

Dieter Langosch

Publication

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Gamma-secretase subunits nicastrin and Aph1a

Expression

Yeast

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pYZ959

Plasmid

#192603

Purpose

TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with CEN-URA3

Depositor

Jef Boeke

Publication

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Promoter

ScTEF1

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ240: pRS426-His-MBP-MmeFz2_Native

Plasmid

#205255

Purpose

S. cerevisiae expression vector for MmeFz2 native guide

Depositor

Feng Zhang

Publication

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

MmeFz2

Tags

10xHis, MBP

Expression

Yeast

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pABY-c46

Plasmid

#201774

Purpose

Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast

Depositor

Saravanan Palani

Publication

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Promoter

TEF1

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ123: pRS426-His-MBP-SpuFz1_PSP1

Plasmid

#205256

Purpose

S. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Publication

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

10xHis, MBP

Expression

Yeast

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pRS305-LEU2-pINO4-SV40NLS-PfV-GS-yEmCherry

Plasmid

#203656

Purpose

Yeast integrative vector for the expression of the yEmCherry-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter.

Depositor

Liam Holt

Publication

Shu T et et al bioRxiv 2022

Insert

PfV

Tags

yEmCherry

Expression

Yeast

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pRS305-mAID-Nb(VHHGFP4)

Plasmid

#198412

Purpose

Express mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeast

Depositor

Takumi Kamura

Publication

Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

Insert

mAID-Nb (VHHGFP4)

Expression

Yeast

Promoter

ADH1 promoter

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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GAL-Aur1-2GFP-Hygromycin

Plasmid

#184775

Purpose

Yeast expression of Aur1 under GAL promoter. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

proGAL-Aur1 (AUR1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGAL-flp(H305L)-NAT

Plasmid

#160380

Purpose

overexpression of Flp recombinase step-arrest mutant in yeast

Depositor

John McCusker

Insert

FLP1

Expression

Yeast

Mutation

H305L

Promoter

GAL

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGAL-SKI1-NAT

Plasmid

#160382

Purpose

Overxpression of SKI1/XRN1 in yeast

Depositor

John McCusker

Insert

SKI1

Expression

Yeast

Promoter

GAL

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGal-BE3

Plasmid

#172409

Purpose

Yeast plasmid encoding the third-generation CRISPR base editor BE3 (Komor et al., Nature 2016) under a galactose-inducible promoter

Depositor

Leonid Kruglyak

Publication

Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

Insert

BE3

Use

CRISPR

Expression

Yeast

Promoter

GALL

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pEasyG1_nat

Plasmid

#184911

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Publication

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pWS3866

Plasmid

#185743

Purpose

Sub-array backbone 2/4

Depositor

Rodrigo Ledesma-Amaro

Publication

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Expression

Yeast

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSAC200

Plasmid

#187911

Purpose

psac200-crispey-bar-vector

Depositor

Hunter Fraser

Publication

Chen et al Cell Genom. 2023 Feb 28;3(4):100273. doi

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pSAC212

Plasmid

#187912

Purpose

psac212-crispey-bar-ade2KO

Depositor

Hunter Fraser

Publication

Chen et al Cell Genom. 2023 Feb 28;3(4):100273. doi

Insert

ade2 KO CRISPEY cassette

Use

CRISPR

Expression

Yeast

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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