8,265 results
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Plasmid#241276PurposeYeast two hybrid vector expressing AD-HAB1DepositorAvailable SinceSept. 8, 2025AvailabilityAcademic Institutions and Nonprofits only
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pBD_PYR1_156-F1
Plasmid#241286PurposeBackbone for PYR1 sensor library assembly from oligopools; stop codon removed; PYR1 sensor library assembly from oligopools;DepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBD_PYR1_156
Plasmid#241285PurposeBackbone for PYR1 sensor library assembly from oligopools; domesticated for golden gate cloningDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBD-PYR1
Plasmid#241278PurposeYeast two hybrid vector expressing BD-PYR1 (wild type)DepositorAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFS557
Plasmid#242857PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetSW1 promoter at its endogenous locusDepositorInsertsTetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW1 promoterAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFS551
Plasmid#242851PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetS promoter at its endogenous locusDepositorInsertsTetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetS promoterAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACT-HAB1-backbone
Plasmid#241287PurposeBackbone for HAB1 mutants library construction from oligopools; PYR1 sensor library assembly from oligopools;DepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 Cyterm-mGold
Plasmid#224084PurposeExpresses Cyterm-mGold to check for monomericity.DepositorInsertCyterm-mGold
ExpressionYeastAvailable SinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-psfGFPcp-natMX6
Plasmid#240557PurposeTag S. pombe genes at 3' end (C-terminal end of protein) with circularly permuted superfolder GFP, codon-optimized for S. pombeDepositorInsertpsfGFPcp
ExpressionYeastAvailable SinceAug. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGADT7_Sr62TK-Kinase1
Plasmid#233529PurposeExpresses Sr62TK-Kinase1 in yeastDepositorInsertSr62TK-Kinase1
TagsGAL4 AD, HA tagExpressionYeastPromoterADH1Available SinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
Sc Pol12-pRS406/GAL
Plasmid#241976PurposeOver-express Sc Pol12 (Sc pol alpha subunit) in yeast (integrated)DepositorInsertPol12 (POL12 Budding Yeast)
ExpressionYeastAvailable SinceAug. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Sc FLAG-Rev1-pRS425/GAL
Plasmid#241979PurposeOver-express Sc FLAG-Rev1 in yeast (episomal)DepositorAvailable SinceAug. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Sc FLAG-Pol2-pRS425/GAL
Plasmid#241978PurposeOver-express Sc FLAG-Pol2 (Pol epsilon large subunit) in yeast (episomal)DepositorAvailable SinceAug. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Sc Pol1-FLAG-pRS402/GAL-L
Plasmid#241975PurposeOver-express Sc Pol1-FLAG (Sc pol alpha - large subunit) in yeast (integrated)DepositorAvailable SinceAug. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Sc dpb11-FLAG-pRS405/GAL
Plasmid#240739PurposeOver-express Sc dpb11-FLAG in Yeast (integrated)DepositorAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Sc mec1-S6-P/FLAG-pRS403/GAL-L
Plasmid#240737PurposeOver-express Sc mec1-S6-PreScission Protease Site-FLAG in yeast (integrated)DepositorInsertmec1-S6-P/FLAG (MEC1 Budding Yeast)
TagsS6, followed by PreScission Protease site, follow…ExpressionYeastAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Sc FLAG-rad24-pRS404/GAL-L
Plasmid#240735PurposeOver-express FLAG-rad24 in yeast (integrated)DepositorAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Sc FLAG-S6-rad24- pRS404/GAL-L
Plasmid#240736PurposeOver-express FLAG-S6-rad24 in yeast (integrated)DepositorAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Sc ddc2-pRS404/GAL-U
Plasmid#240738PurposeOver-express Sc ddc2 in yeast (integrated)DepositorInsertddc2
ExpressionYeastAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Sc FLAG-POL4-pRS404/GAL-L
Plasmid#241261PurposeOver-express Sc FLAG-POL4 in yeast (integrated)DepositorAvailable SinceAug. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Sc FLAG-rad30-pRS403/GAL
Plasmid#241260PurposeOver-express Sc FLAG-rad30 (Sc Pol eta) in yeast (integrated)DepositorAvailable SinceAug. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Sc rev7-pRS405/GAL
Plasmid#241259PurposeOver-express Sc rev7 (Sc Pol zeta subunit) in yeast (integrated)DepositorInsertrev7 (REV7 Budding Yeast)
ExpressionYeastAvailable SinceAug. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Sc rev3-FLAG-pRS402/GAL
Plasmid#241258PurposeOver-express Sc rev3-FLAG (Sc Pol zeta subunit) in yeast (integrated)DepositorAvailable SinceAug. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Aga2p-TEVcs-LaccID-myc_pCTCON2
Plasmid#234049Purposeexpresses LaccID on the yeast surfaceDepositorInsertAga2p-LaccID
ExpressionYeastAvailable SinceAug. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVL1710
Plasmid#239543PurposeExpress Yea4p membrane protein cargo fused to C-terminal Venus and TEV site, GAL-inducible TEV protease, and Pex22 peroxisomal chauffeurDepositorInsertScYea4p-2xCrk_SH3-mTurq2-TEVsite-Venus
ExpressionYeastAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1614
Plasmid#239545PurposeExpress truncated VoGES, Idi1, and Erg20WW for synthesis of geraniol in the peroxisome with Venus as peroxisomal markerDepositorInserttVoGES-ePTS1
ExpressionYeastAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVL1708
Plasmid#239542PurposeExpress Yea4p membrane protein cargo fused to N-terminal Venus and TEV site, GAL-inducible TEV protease, and Pex22 peroxisomal chauffeurDepositorInsertVenus-TEVsite-ScYea4p-mTurq2-2xCrk_SH3
ExpressionYeastAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLS406_YCR051W
Plasmid#239133PurposeMinimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker URA3DepositorTypeEmpty backboneUseIntegratingExpressionBacterial and YeastAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLS408_YCR051W
Plasmid#239134PurposeMinimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker natMX6DepositorTypeEmpty backboneUseIntegratingExpressionBacterial and YeastAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30-LaccID
Plasmid#234652PurposeExpresses LaccID evolved variant in yeastDepositorInsertLaccID
TagsHRV 3C protease site-GSG linker-8xHis tagExpressionYeastMutationV11A, Q45R, N74S, R121S, I126V, D255G, L300VPromoterGal1pAvailable SinceAug. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30-LacAnc100
Plasmid#234653PurposeExpresses LacAnc100 variant in yeastDepositorInsertLacAnc100
TagsHRV 3C protease site-GSG linker-8xHis tagExpressionYeastPromoterGal1pAvailable SinceAug. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
Sc FLAG-Rev1-pRS404/GAL
Plasmid#241980PurposeOver-express Sc FLAG-Rev1 in yeast (integrated)DepositorAvailable SinceJuly 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-pmNeonGreen-hphNT1
Plasmid#240550PurposeTag S. pombe genes at 3' end (C-terminal end of protein) with mNeonGreen, codon-optimized for S. pombeDepositorInsertpmNeonGreen
ExpressionYeastAvailable SinceJuly 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-pTagRFP-T-hphNT1
Plasmid#240556PurposeTag S. pombe genes at 3' end (C-terminal end of protein) with TagRFP-T, codon-optimized for S. pombeDepositorInsertpTagRFP-T
ExpressionYeastAvailable SinceJuly 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-psfGFP-kanMX6
Plasmid#240551PurposeTag S. pombe genes at 3' end (C-terminal end of protein) with superfolder GFP, codon-optimized for S. pombeDepositorInsertpsfGFP
ExpressionYeastAvailable SinceJuly 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-pTagRFP-T-kanMX6
Plasmid#240555PurposeTag S. pombe genes at 3' end (C-terminal end of protein) with TagRFP-T, codon-optimized for S. pombeDepositorInsertpTagRFP-T
ExpressionYeastAvailable SinceJuly 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-psfGFPcp-hphNT1
Plasmid#240558PurposeTag S. pombe genes at 3' end (C-terminal end of protein) with circularly permuted superfolder GFP, codon-optimized for S. pombeDepositorInsertpsfGFPcp
ExpressionYeastAvailable SinceJuly 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-pmKate2-hphNT1
Plasmid#240554PurposeTag S. pombe genes at 3' end (C-terminal end of protein) with mKate2, codon-optimized for S. pombeDepositorInsertpmKate2
ExpressionYeastAvailable SinceJuly 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-pmNeonGreen-kanMX6
Plasmid#240549PurposeTag S. pombe genes at 3' end (C-terminal end of protein) with mNeonGreen, codon-optimized for S. pombeDepositorInsertpmNeonGreen
ExpressionYeastAvailable SinceJuly 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-pmKate2-kanMX6
Plasmid#240553PurposeTag S. pombe genes at 3' end (C-terminal end of protein) with mKate2, codon-optimized for S. pombeDepositorInsertpmKate2
ExpressionYeastAvailable SinceJuly 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-psfGFP-hphNT1
Plasmid#240552PurposeTag S. pombe genes at 3' end (C-terminal end of protein) with superfolder GFP, codon-optimized for S. pombeDepositorInsertpsfGFP
ExpressionYeastAvailable SinceJuly 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1603
Plasmid#239527PurposeExpress CpPT1(52-408) membrane enzyme cargo and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPRDepositorInsert2xCrk_SH3-mTurquoise2-CpPT1(52-408)
ExpressionYeastAvailable SinceJuly 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVL1173
Plasmid#239536PurposeExpress CpPT1 membrane protein cargo with N-terminal mTurqoise2 marker but without any targeting sequence and Venus ER markerDepositorInsertmTurquoise2-CpPT1(52-408)
ExpressionYeastAvailable SinceJuly 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1616
Plasmid#239547PurposeExpress GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPRDepositorInsertCrG8H-2xCrk_SH3
ExpressionYeastAvailable SinceJuly 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pN4
Plasmid#233521PurposeNb Vector Plasmid (under galactose induction), AmpR (E. coli) and TRP1 (yeast), Cen6/ARSDepositorTypeEmpty backboneExpressionBacterial and YeastPromoterpGAL1-10Available SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pY3-NbLibRec
Plasmid#233522PurposeNb Library Receiver Vector (under galactose induction), AmpR (E. coli) and TRP1 (yeast), Cen6/ARSDepositorTypeEmpty backboneExpressionBacterial and YeastPromoterpGAL1-10Available SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2618
Plasmid#234497PurposeNEOR-CnU6-empty sgRNADepositorInsertNEO-PCnU6-sgRNA_scaffold
UseCRISPRExpressionBacterial and YeastAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2661
Plasmid#234500PurposeLinker-Cno_mNeonGreen-CBP-2xFLAG-CnU6-sgRNA-NEORDepositorInsertmNeonGreen-CBP-2XFLAG-NEO-PCnU6-sgRNA_scaffold
UseCRISPRExpressionBacterial and YeastAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2616
Plasmid#234495PurposeHYGR-CnU6-empty sgRNADepositorInsertHYG-PCnU6-sgRNA_scaffold
UseCRISPRExpressionBacterial and YeastAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1429
Plasmid#239514PurposeExpress Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative controlDepositorInsertScYea4p-mTurquoise2-2xCrk_SH3
ExpressionYeastAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only