7,935 results

1. pFA6a-FLAG-APEX2-NES-HIS3

   Plasmid

   #229230

   Purpose

   Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

   Depositor

   Fulvio Reggiori

   Article

   Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRS413-CUP1pr-NFAPoptim

   Plasmid

   #221110

   Purpose

   FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRS413-CUP1pr-CFAPoptim

   Plasmid

   #221111

   Purpose

   FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRS415-TEF1pr-MFA-Igkappa-FAPoptim-STE3

   Plasmid

   #221114

   Purpose

   FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Tef1 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   MFA-IgKappa-FAPoptim-STE3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-deltaPEST

   Plasmid

   #221118

   Purpose

   FAP tagged STE3-PEST mutant (delta413-470 - N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   MFA-IgKappa-FAPoptim-STE3-deltaPEST
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   STE3 mutant that lacks the PEST domain in the C-t…

   Promoter

   STE3

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-K424R

   Plasmid

   #221119

   Purpose

   FAP tagged STE3-K424R mutant (N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   MFA-IgKappa-FAPoptim-STE3-K424R
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   STE3 mutantK424R

   Promoter

   STE3

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS413-TEF1pr-ANP1-FAPoptim

   Plasmid

   #221120

   Purpose

   FAP-tagged marker for the cis-Golgi (FAP optimized for yeast expression)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   ANP1-FAPoptim
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRS413-TEF1pr-ERG6-FAPoptim

   Plasmid

   #221121

   Purpose

   FAP-tagged marker for lipid droplets (FAP optimized for yeast expression)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   ERG6-FAPoptim
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS413-TEF1pr-PMA1-FAPoptim

   Plasmid

   #221122

   Purpose

   FAP-tagged marker for the plasma membrane (FAP optimized for yeast expression)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   PMA1-FAPoptim
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRS413-TEF1pr-RPA34-FAPoptim

    Plasmid

    #221123

    Purpose

    FAP-tagged marker for the nucleus (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    RPA34-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRS413-TEF1pr-SEC7-FAPoptim

    Plasmid

    #221124

    Purpose

    FAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    SEC7-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRS413-TEF1pr-VPH1-FAPoptim

    Plasmid

    #221125

    Purpose

    FAP-tagged marker for the vacuolar membrane (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    VPH1-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRS415-TEF1pr-NFAPoptim

    Plasmid

    #221088

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRS415-TEF1pr-CFAPoptim

    Plasmid

    #221089

    Purpose

    FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS415-TEF1pr-MFA-NFAPoptim

    Plasmid

    #221090

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS413-TEF1pr-NFAPorig

    Plasmid

    #221091

    Purpose

    FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS413-TEF1pr-NFAPoptim

    Plasmid

    #221093

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS413-TEF1pr-CFAPoptim

    Plasmid

    #221094

    Purpose

    FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS415-ADH1pr-NFAPoptim

    Plasmid

    #221096

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS415-ADH1pr-CFAPoptim

    Plasmid

    #221097

    Purpose

    FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRS413-ADH1pr-NFAPoptim

    Plasmid

    #221098

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRS413-ADH1pr-CFAPoptim

    Plasmid

    #221099

    Purpose

    FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)+E2:E15

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRS415-MET25pr

    Plasmid

    #221100

    Purpose

    Methionine-repressible gene expression

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRS415-MET25pr-NFAPoptim

    Plasmid

    #221101

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRS415-MET25pr-CFAPoptim

    Plasmid

    #221102

    Purpose

    FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRS413-MET25pr

    Plasmid

    #221103

    Purpose

    Methionine-repressible gene expression

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRS413-MET25pr-NFAPoptim

    Plasmid

    #221104

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRS413-MET25pr-CFAPoptim

    Plasmid

    #221105

    Purpose

    FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRS415-CUP1pr

    Plasmid

    #221106

    Purpose

    Copper-inducible gene expression

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRS415-CUP1pr-NFAPoptim

    Plasmid

    #221107

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRS415-CUP1pr-CFAPoptim

    Plasmid

    #221108

    Purpose

    FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRS415-TEF1pr-NFAPorig

    Plasmid

    #221086

    Purpose

    FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRS415-TEF1pr-CFAPorig

    Plasmid

    #221087

    Purpose

    FAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJL-mGold2t

    Plasmid

    #231762

    Purpose

    Expression of mGold2t in yeast cells

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

    Promoter

    pTDH(GAP promoter)

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. mP_ILB_Vector 1

    Plasmid

    #232193

    Purpose

    The plasmid vector designed for the expression of four genes in oleaginous yeasts, including Yarrowia lipolytica and Rhodotorula toruloides.

    Depositor

    Matrika Bhattarai

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 3, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. MPS1 Candia parapsilosis

    Plasmid

    #232153

    Purpose

    MPS1 from Candia parapsilosis codon optimized for S. cerevisiae in pUCIDT

    Depositor

    Mark Winey

    Article

    Fabritius et al PLoS One. 2024 Mar 26;19(3):e0301084. do

    Insert

    MPS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. MPS1 Rhizoclosmatium globosum

    Plasmid

    #232152

    Purpose

    MPS1 from Rhizoclosmatium globosum codon optimized for S. cerevisiae in pUCIDT

    Depositor

    Mark Winey

    Article

    Fabritius et al PLoS One. 2024 Mar 26;19(3):e0301084. do

    Insert

    MPS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MPS1 Malassezia globosa

    Plasmid

    #232151

    Purpose

    MPS1 from Malassezia globosa codon optimized for S. cerevisiae in pUCIDT

    Depositor

    Mark Winey

    Article

    Fabritius et al PLoS One. 2024 Mar 26;19(3):e0301084. do

    Insert

    MPS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Likely not full protein

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MPS1 Candida auris

    Plasmid

    #232150

    Purpose

    MPS1 from Candida auris codon optimized for S. cerevisiae in pUCIDT

    Depositor

    Mark Winey

    Article

    Fabritius et al PLoS One. 2024 Mar 26;19(3):e0301084. do

    Insert

    MPS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MPS1 Human

    Plasmid

    #232149

    Purpose

    MPS1 from Human codon optimized for S. cerevisiae in pUCIDT

    Depositor

    Mark Winey

    Article

    Fabritius et al PLoS One. 2024 Mar 26;19(3):e0301084. do

    Insert

    MPS1 (TTK Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. MPS1 Candida glabrata

    Plasmid

    #232148

    Purpose

    MPS1 from Candida glabrata codon optimized for S. cerevisiae in pUCIDT

    Depositor

    Mark Winey

    Article

    Fabritius et al PLoS One. 2024 Mar 26;19(3):e0301084. do

    Insert

    MPS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. MPS1 Schizosaccharomyces pombe

    Plasmid

    #232147

    Purpose

    MPS1 from Schizosaccharomyces pombe codon optimized for S. cerevisiae in pUCIDT

    Depositor

    Mark Winey

    Article

    Fabritius et al PLoS One. 2024 Mar 26;19(3):e0301084. do

    Insert

    MPS1 (mph1 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. MPS1 Candida albicans

    Plasmid

    #232146

    Purpose

    MPS1 from Candida albicans codon optimized for S. cerevisiae in pUCIDT

    Depositor

    Mark Winey

    Article

    Fabritius et al PLoS One. 2024 Mar 26;19(3):e0301084. do

    Insert

    MPS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pML107-RER2

    Plasmid

    #232884

    Purpose

    Plasmid expressing Cas9 and gRNA AGAATCGCATCTCTACACGG which targets the RER2 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    RER2 gRNA (RER2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pML104-LEU2-3

    Plasmid

    #232894

    Purpose

    Plasmid expressing Cas9 and gRNA GTTCTTAACTAGGATCATGG which targets the RER2 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    LEU2 gRNA (RER2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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46. pRS313_MSN5-LY00045

    Plasmid

    #232904

    Purpose

    Plasmid carrying MSN5 from LY00045 (S288C) with its native promoter and terminator.

    Depositor

    Jolanda Van Leeuwen

    Insert

    MSN5 (MSN5 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. pRS313_MSN5-LY00018

    Plasmid

    #232903

    Purpose

    Plasmid carrying MSN5 from LY00018 (YJM975) with its native promoter and terminator.

    Depositor

    Jolanda Van Leeuwen

    Insert

    MSN5 (MSN5 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    G1563A

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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48. pML104-HygMx4-URA3-3

    Plasmid

    #232886

    Purpose

    Plasmid expressing Cas9 and gRNA GAAGTAACAAAGGAACCTAG which targets the URA3 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    URA3 gRNA (URA3 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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49. pML104-HygMx4-URA3-2

    Plasmid

    #232885

    Purpose

    Plasmid expressing Cas9 and gRNA TCGTACCACCAAGGAATTAC which targets the URA3 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    URA3 gRNA (URA3 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCRISPEY-Z3-Kan

    Plasmid

    #232102

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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