8,173 results
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Plasmid#234134PurposeAmino acids 103-173 deleted in Fyv6 with FLAG epitope tag at N-terminusDepositorInsertFyv6
ExpressionYeastMutationC-terminus truncationAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Fyv6-CFLAG
Plasmid#234133PurposeRestriction digest from pUC18-CFLAG-Fyv6 and ligation into pRS414DepositorInsertFyv6
ExpressionYeastAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Fyv6-NFLAG del134-173
Plasmid#234135PurposeAmino acids 134-173 deleted in Fyv6 with FLAG epitope tag at N-terminusDepositorInsertFyv6
ExpressionYeastMutationC-terminus truncationAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Fyv6
Plasmid#234140PurposeAmplified Fyv6 from gDNA, cut with SalI and NotI, ligated into pRS414DepositorInsertFyv6
ExpressionYeastAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-NFLAG-Fyv6
Plasmid#234139PurposeRestriction digest from pUC18-NFLAG-Fyv6 and ligation into pRS414DepositorInsertFyv6
ExpressionYeastAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS424 Prp8 G1868R
Plasmid#234124PurposeSuppressor screen mutant in full-length Prp8 on a TRP plasmidDepositorInsertPrp8
ExpressionYeastMutationG1868RAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Fyv6
Plasmid#234141PurposeAmplified Fyv6 from gDNA, cut with SalI and NotI, ligated into pRS416DepositorInsertFyv6
ExpressionYeastAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Fyv6-NFLAG del1-23
Plasmid#234137PurposeFirst 23 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminusDepositorInsertFyv6
ExpressionYeastMutationN-terminus truncationAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS314-Cef1-M175I
Plasmid#234118PurposeSuppressor screen mutant in Cef2DepositorInsertCef1
ExpressionYeastMutationM175IAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS314-Cef1-Q193P
Plasmid#234117PurposeSuppressor screen mutant in Cef1DepositorInsertCef1
ExpressionYeastMutationQ193PAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS314-Cef1-A37V
Plasmid#234119PurposeSuppressor screen mutant in Cef3DepositorInsertCef1
ExpressionYeastMutationA37VAvailable SinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Syf1
Plasmid#234113PurposeSyf1 region PCR, restriction digest and ligation into pRS416 backboneDepositorInsertSyf1
ExpressionYeastAvailable SinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Syf1 634 Truncation
Plasmid#234116PurposeSyf1 PCR truncation starting at 634 through the end of the geneDepositorInsertSyf1
ExpressionYeastMutationN-terminus truncationAvailable SinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGADT7_Sr62NLRa
Plasmid#233528PurposeExpresses Sr62NLRa in yeastDepositorInsertSr62NLRa
TagsGAL4 AD, HA tagExpressionYeastPromoterADH1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Syf1 778 Truncation
Plasmid#234115PurposeSyf1 PCR truncation starting at 778 through the end of the geneDepositorInsertSyf1
ExpressionYeastMutationN-terminus truncationAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 WTSLFD
Plasmid#232980PurposeGalactose iduced expression of Gcn4 WTSLFD+ in yeastDepositorInsertGcn4 WTSLFD+
Tags3xHA and TEV cleavage siteExpressionYeastMutationF108W, E109T, Y110S, E111L, N112F, L113DPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro2+
Plasmid#232982PurposeGalactose iduced expression of Gcn4 Aro2+ in yeastDepositorInsertGcn4 Aro2+
TagsTEV cleavage siteExpressionYeastMutationN116F, S117E, E119N, P129Q, T132YPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro3+
Plasmid#232983PurposeGalactose iduced expression of Gcn4 Aro3+ in yeastDepositorInsertGcn4 Aro3+
TagsTEV cleavage siteExpressionYeastMutationD103Y, N112T, L113Y, S117F, K140RPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 W120AHA
Plasmid#232992PurposeGalactose iduced expression of Gcn4 W120AHA in yeastDepositorInsertGcn4 W120A
Tags3xHA and TEV cleavage siteExpressionYeastMutationW120APromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 SATtoG KtoRHA
Plasmid#232994PurposeGalactose iduced expression of Gcn4 SATtoG KtoRHAin yeastDepositorInsertGcn4 SATtoG KtoR
Tags3xHA and TEV cleavage siteExpressionYeastMutationS101G, T102G, S104G, T105G, S117G, K118R, T121G, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro3+HA
Plasmid#232990PurposeGalactose iduced expression of Gcn4 Aro3+HA in yeastDepositorInsertGcn4 Aro3+
Tags3xHA and TEV cleavage siteExpressionYeastMutationD103Y, N112T, L113Y, S117F, K140RPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_DBDHA
Plasmid#232987PurposeGalactose iduced expression of Gcn4 DBDAHA in yeastDepositorInsertDBD
Tags3xHA and TEV cleavage siteExpressionYeastPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 WT 44merHA
Plasmid#232988PurposeGalactose iduced expression of Gcn4 WT 44merHA in yeastDepositorInsertGcn4 WT 44mer
Tags3xHA and TEV cleavage siteExpressionYeastPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro2+HA
Plasmid#232989PurposeGalactose iduced expression of Gcn4 Aro2+HA in yeastDepositorInsertGcn4 Aro2+
Tags3xHA and TEV cleavage siteExpressionYeastMutationN116F, S117E, E119N, P129Q, T132YPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 F124IGFP
Plasmid#233005PurposeGalactose iduced expression of Gcn4 F124IGFP in yeastDepositorInsertGcn4 F124I
TagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationF124IPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 WW+HA
Plasmid#232991PurposeGalactose iduced expression of Gcn4 WW+HA in yeastDepositorInsertGcn4 WW+
Tags3xHA and TEV cleavage siteExpressionYeastMutationE109N, S117D, T121W, N126W, T132WPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 L123A
Plasmid#232969PurposeGalactose iduced expression of Gcn4 L123A in yeastDepositorInsertGcn4 L123A
TagsTEV cleavage siteExpressionYeastMutationL123APromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 SATtoG KtoR
Plasmid#232961PurposeGalactose iduced expression of Gcn4 ATtoG KtoR in yeastDepositorInsertGcn4 SATtoG KtoR
TagsTEV cleavage siteExpressionYeastMutationS101G, T102G, S104G, T105G, S117G, K118R, T121G, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Swap2
Plasmid#232965PurposeGalactose iduced expression of Gcn4 k0.19 in yeastDepositorInsertGcn4 k0.19
TagsTEV cleavage siteExpressionYeastPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 WW+GFP
Plasmid#233003PurposeGalactose iduced expression of Gcn4 WW+GFP in yeastDepositorInsertGcn4 WW+
TagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationE109N, S117D, T121W, N126W, T132WPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 W120AGFP
Plasmid#233004PurposeGalactose iduced expression of Gcn4 120AGFP in yeastDepositorInsertGcn4 W120A
TagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationW120APromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro2+GFP
Plasmid#233001PurposeGalactose iduced expression of Gcn4 Aro2+GFP in yeastDepositorInsertGcn4 Aro2+
TagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationN116F, S117E, E119N, P129Q, T132YPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 solvvol
Plasmid#232976PurposeGalactose iduced expression of Gcn4 solvvol in yeastDepositorInsertGcn4 solvvol
TagsTEV cleavage siteExpressionYeastMutationS101T, D103E, S104T, D115E, N116Q, S117T, S122T, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 S/ArotoL
Plasmid#232979PurposeGalactose iduced expression of Gcn4 S/ArotoL in yeastDepositorInsertGcn4 S/ArotoL
TagsTEV cleavage siteExpressionYeastMutationS101L, S104L, F108L, Y110L, S117L, S136L, S144LPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 K5
Plasmid#232966PurposeGalactose iduced expression of Gcn4 K+ in yeastDepositorInsertGcn4 K+
TagsTEV cleavage siteExpressionYeastMutationD103K, D115K, D127K, D133K, E143KPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 ILVtoED solvvol
Plasmid#232958PurposeGalactose iduced expression of Gcn4 LVtoED solvvol in yeastDepositorInsertGcn4 ILVtoED solvvol
TagsTEV cleavage siteExpressionYeastMutationS101T, D103E, S104T, D115E, N116Q, S117T, S122T, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Swap3
Plasmid#232960PurposeGalactose iduced expression of Gcn4 k0.47 in yeastDepositorInsertGcn4 k0.47
TagsTEV cleavage siteExpressionYeastPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 R2
Plasmid#232970PurposeGalactose iduced expression of Gcn4 R+ in yeastDepositorInsertGcn4 R+
TagsTEV cleavage siteExpressionYeastMutationK118R, K140RPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 E+
Plasmid#232963PurposeGalactose iduced expression of Gcn4 E+in yeastDepositorInsertGcn4 E+
TagsTEV cleavage siteExpressionYeastMutationD105E, D118E, D139EPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 W120A
Plasmid#232962PurposeGalactose iduced expression of Gcn4 W120A in yeastDepositorInsertGcn4 W120A
TagsTEV cleavage siteExpressionYeastMutationW120APromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 P+
Plasmid#232971PurposeGalactose iduced expression of Gcn4 Proline in yeastDepositorInsertGcn4 Proline
TagsTEV cleavage siteExpressionYeastMutationS122P, T131F, A141P, E143DPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 R3
Plasmid#232973PurposeGalactose iduced expression of Gcn4 R++ in yeastDepositorInsertGcn4 R++
TagsTEV cleavage siteExpressionYeastMutationD103R, D133R, K143RPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Swap3GFP
Plasmid#233010PurposeGalactose iduced expression of Gcn4 k0.47GFP in yeastDepositorInsertGcn4 k0.47
TagsHA-GFP-HA and TEV cleavage siteExpressionYeastPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 ILVtoED WHA
Plasmid#232995PurposeGalactose iduced expression of Gcn4 ILVtoED W+HAin yeastDepositorInsertGcn4 ILVtoED W+
Tags3xHA and TEV cleavage siteExpressionYeastMutationN126W, I128E, V130E, V135E, L137D, I142DPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 9acidAHA
Plasmid#232997PurposeGalactose iduced expression of Gcn4 9acidAHA in yeastDepositorInsertGcn4 9acidA
Tags3xHA and TEV cleavage siteExpressionYeastMutationE109A, E111A, E114A, D115A, E119A, D125A, D127A, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_DBD
Plasmid#232985PurposeGalactose iduced expression of Gcn4 DBD in yeastDepositorInsertGcn4 DBD
TagsTEV cleavage siteExpressionYeastPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 F124I
Plasmid#232964PurposeGalactose iduced expression of Gcn4 F124I in yeastDepositorInsertGcn4 F124I
TagsTEV cleavage siteExpressionYeastMutationF124IPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 9acidA
Plasmid#232967PurposeGalactose iduced expression of Gcn4 9acidA in yeastDepositorInsertGcn4 9acidA
TagsTEV cleavage siteExpressionYeastMutationE109A, E111A, E114A, D115A, E119A, D125A, D127A, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 E2K2
Plasmid#232968PurposeGalactose iduced expression of Gcn4 EK+ in yeastDepositorInsertGcn4 EK+
TagsTEV cleavage siteExpressionYeastMutationS104K, S117E, S136E, S144KPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 9acidAGFP
Plasmid#233009PurposeGalactose iduced expression of Gcn4 9acidAGFP in yeastDepositorInsertGcn4 9acidA
TagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationE109A, E111A, E114A, D115A, E119A, D125A, D127A, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only