8,432 results

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p416TEF-SEC16

Plasmid

#102676

Purpose

The gene SEC16 is driven by the strong promoter TEF. Overexpression of SEC16 increases recombinant protein secretion

Depositor

Jens Nielsen

Publication

Bao et al Appl Environ Microbiol. 2017 Jun 30;83(1

Insert

SEC16 (SEC16 Budding Yeast)

Expression

Yeast

Promoter

TEF

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCTcon2-5INS_EV3

Plasmid

#102293

Purpose

Yeast Surface Display of 5Ins_16_EV3

Depositor

Sarel Fleishman

Publication

Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

Insert

Evolved Insulin binding scFV

Tags

3x(G4S) Linker, G3S Linker, HA, and Myc

Expression

Yeast

Promoter

GAL1

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pDR FLIP43 GPS1-NR

Plasmid

#101085

Purpose

GA biosensor expression plasmid

Depositor

Wolf Frommer, Alexander Jones

Publication

Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

Insert

GPS1-NR

Tags

6HIS and cMyc

Expression

Yeast

Mutation

AtGAI E51K E54R, AtGID1C S114A F115A

Promoter

pPMA1

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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YIp211-P30-His-POL30-3'

Plasmid

#99546

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA); URA3 marker

Depositor

Helle Ulrich

Publication

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

POL30

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR221-Sen1

Plasmid

#99994

Purpose

Shuttle yeast Sen1 into Gateway destination vector

Depositor

Susan Baserga

Publication

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Sen1 (SEN1 Budding Yeast)

Expression

Yeast

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pJK148-Pnmt41-GBP-mCherry(C)-leu1+

Plasmid

#89069

Purpose

genomic GBP-mCherry epitope C-terminal tagging at leu1+ locus in S. pombe

Depositor

Quanwen Jin

Publication

Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

Type

Empty backbone

Use

Genomic integration in s. pombe

Tags

GBP-mCherry

Expression

Yeast

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pJT1

Plasmid

#98299

Purpose

Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter

Depositor

Claudia Vickers

Publication

Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

Insert

PTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B

Expression

Yeast

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pLK88 - Edit-directing plasmid base

Plasmid

#98814

Purpose

Edit-directing plasmid base

Depositor

Leonid Kruglyak

Insert

CAN1.y gRNA

Use

CRISPR

Expression

Yeast

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC119- Padh81-GBP-mCherry(C)-hphMX6-lys1*

Plasmid

#89071

Purpose

genomic GBP-mCherry epitope C-terminal tagging at lys1+ locus in S. pombe

Depositor

Quanwen Jin

Publication

Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

Type

Empty backbone

Use

Genomic integration in s. pombe

Tags

GBP-mCherry

Expression

Yeast

Available since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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pH3FS-PYK1

Plasmid

#85781

Purpose

N-ICE plasmid pH3FS-PYK1

Depositor

Scott Briggs

Publication

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pDRf1-4CL5-DsRed (JBEI-15461)

Plasmid

#87935

Purpose

Cloning vector for co-expressing a gene with 4CL5 in S.cerevisiae

Depositor

Dominique Loque

Publication

Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK020-7-attB

Plasmid

#84989

Purpose

Type 7, BxbI recognition site

Depositor

Volker Sieber

Publication

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

BxbI recognition site

Use

Synthetic Biology

Expression

Yeast

Mutation

BsaI site removed (1711 t to a)

Promoter

Non

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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p426TEF-LptTPS-A

Plasmid

#89468

Purpose

Expression of prespatane synthase LptTPS-A in S. cerevisiae for sesquiterpene production

Depositor

Jing-Ke Weng

Insert

LptTPS-A

Tags

none

Expression

Yeast

Promoter

pBR322 origin of replication for propagation in E…

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK009-3a-αAmylase-αMFΔ

Plasmid

#84968

Purpose

Type 3a, α-Amylase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Publication

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-Amylase followed by MFΔ secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK018-3b-RFP_Sec

Plasmid

#84977

Purpose

Type 3b, Red fluorescent protein

Depositor

Volker Sieber

Publication

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Red fluorescent protein

Use

Synthetic Biology

Expression

Yeast

Mutation

BsaI site removed (1677 c to g and 1794 a to g)

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pJB166

Plasmid

#86998

Purpose

CRISPR/Cas9 in fission yeast using fluoride selection

Depositor

Julien Berro

Publication

Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pK3F

Plasmid

#85770

Purpose

N-ICE plasmid pK3F

Depositor

Scott Briggs

Publication

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-3FLAG

Use

Cre/Lox

Expression

Yeast

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pILGFPH7

Plasmid

#83554

Purpose

Used to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Publication

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

ADH2 promoter-yEGFP

Expression

Yeast

Promoter

ADH2

Available since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB1975 (pUC19-PTEF1-PPGK1)

Plasmid

#73296

Purpose

Example plasmid containing a bidirectional promoter

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

pTEF1-pPGK1

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB3034(X-3 MarkerFree)

Plasmid

#73272

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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