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pDONR221_SLC43A1

Plasmid

#132178

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC43A1 (SLC43A1 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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mEmerald-MPO-N-18

Plasmid

#54187

Purpose

Localization: Lysosomes, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

MPO (MPO Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mIFP12-Fibrillarin-7

Plasmid

#56252

Purpose

Localization: Nuclear - Nucleolus, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Fibrillarin (FBL Human)

Tags

mIFP12

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pscAAV-CBh-aSyn-WPRE3-enSV40pA

Plasmid

#194244

Purpose

Expresses human alpha-Synuclein WT

Depositor

Michael J Fox Foundation MJFF

Insert

aSynuclein(WT) (SNCA Human)

Use

AAV

Promoter

CBh

Available Since

Jan. 17, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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3XFlag pCMV5-TOPO TAZ (106∂)

Plasmid

#24814

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of aa 1-106

Available Since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pMX-HA-UbvG08-IRES-GFP

Plasmid

#75030

Purpose

Retroviral vector with ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53)

Depositor

Daniel Durocher

Article

Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

Insert

Ubiquitin

Use

Retroviral

Tags

HA and IRES-eGFP

Mutation

UbvG08 with I44A mutation and no terminal Glycines

Available Since

June 30, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC37A3_STOP

Plasmid

#161273

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC37A3 (SLC37A3 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CD Ugi3

Plasmid

#177195

Purpose

Greengate entry vector pGGC000 modified to contain bladderwort insulator 3

Depositor

Wayne Parrott

Article

Park et al bioRxiv 2021.11.04.467280

Insert

Utricularia gibba insulator 3

Expression

Bacterial

Available Since

June 28, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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PS-CFP2-TBRII-N-18

Plasmid

#57249

Purpose

Localization: Focal Adhesions, Excitation: 400 / 490, Emission: 468 / 511

Depositor

Michael Davidson

Insert

TBRII (TGFBR2 Human)

Tags

PS-CFP2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-Cre sgAmotl2 v3

Plasmid

#158602

Purpose

lenti-viral construct with Cas9, Cre recombinbase and U6 driven sgRNA against mouse Amotl2

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Amotl2 sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK229

Plasmid

#110591

Purpose

BTK Type 3 coding sequence for golden gate assembly and homologous recombination

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Kanamycin resistance

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC35A3

Plasmid

#132135

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35A3 (SLC35A3 Human)

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pPRKCZ-C1-EGFP (C-PRKCZ-C1)

Plasmid

#217759

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Protein kinase C zeta (PRKCZ Human)

Tags

EGFP

Expression

Mammalian

Mutation

C1 domain (aa 123-193)

Promoter

CMV

Available Since

June 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pC009 LshCas13a locus with targeting spacer

Plasmid

#91900

Purpose

LshCas13a locus into pACYC184 with targeting spacer

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LshCas13a locus with targeting spacer

Expression

Bacterial

Available Since

Oct. 16, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK520

Plasmid

#110604

Purpose

BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (spectinomycin resistance)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC18A1_STOP

Plasmid

#161151

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC18A1 (SLC18A1 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Dendra2-Rab4a-7

Plasmid

#57736

Purpose

Localization: Ras GTPase, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Insert

Rab4a (RAB4A Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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tdEos-ARP2-N-14

Plasmid

#57581

Purpose

Localization: Actin Related Protein, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

ARP2 (ACTR2 Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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MTST5

Plasmid

#86602

Purpose

Custom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.

Depositor

Mary Ann Moran

Article

Satinsky et al Methods Enzymol. 2013;531:237-50. doi: 1

Insert

MTST5

Use

Internal standard reverse transcription

Promoter

T7

Available Since

Sept. 15, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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miniSOG-Golgi-7

Plasmid

#57768

Purpose

Localization: Golgi, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Golgi (B4GALT1 Human)

Tags

miniSOG

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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