PurposeContains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60338||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6000
Vector typeYeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesS. cerevisiae (budding yeast)
- Cloning method Restriction Enzyme
- 5′ cloning site hindIII (not destroyed)
- 3′ cloning site xbaI (not destroyed)
- 5′ sequencing primer agt
- 3′ sequencing primer agt (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:UASGAL-Pgal was a gift from Hal Alper (Addgene plasmid # 60338 ; http://n2t.net/addgene:60338 ; RRID:Addgene_60338)
For your References section:Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters. Blazeck J, Garg R, Reed B, Alper HS. Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17. 10.1002/bit.24552 PubMed 22565375