9,684 results

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pC010 LshCas13a locus with nontargeting spacer

Plasmid

#91901

Purpose

LshCas13a locus into pACYC184 with nontargeting spacer

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LshCas13a locus with non-targeting spacer

Expression

Bacterial

Available Since

Oct. 16, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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BAT8 (3RJW)

Plasmid

#28125

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

BAT8 (EHMT2 Human)

Tags

His-tag with integrated thrombin site

Expression

Bacterial

Mutation

contains amino acid residues 913-1193.

Available Since

Feb. 10, 2012

Availability

Industry, Academic Institutions, and Nonprofits
mEmerald-Tropomyosin-C-14

Plasmid

#54289

Purpose

Localization: Actin, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Tropomyosin (TPM1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mRuby-BaxAlpha-C-15

Plasmid

#55847

Purpose

Localization: , Excitation: 558, Emission: 605

Depositor

Michael Davidson

Insert

BaxAlpha

Tags

mRuby

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mTurquoise-H2A-10

Plasmid

#55556

Purpose

Localization: Nucleus/Histones, Excitation: 434, Emission: 474

Depositor

Michael Davidson

Insert

H2A (H2AC13 Human)

Tags

mTurquoise

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Clover2-H2B-C-10

Plasmid

#56378

Purpose

Localization: Nucleus/Histones, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Tags

Clover2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pNIC-TolB-NHSTIIT-GG

Plasmid

#215792

Purpose

Cloning of target gene with N terminal TolB SRP pathway signal peptide N terminal His6 Twin Strep tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK102

Plasmid

#110574

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

T7 promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC30A8_STOP

Plasmid

#161302

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC30A8 (SLC30A8 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pQE-WT-CGG-R12-KIR

Plasmid

#63626

Purpose

CGG-R12-KIR RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

K1F IKR RNAP

Mutation

Q744K, L747V, N748H, L749I, R756E, L757M, H772R

Promoter

T5/Lac

Available Since

July 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mEmerald-Endo-14

Plasmid

#54081

Purpose

Localization: Endosomes, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Endo (RHOB Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pHTN-HaloTag_TRIB1:1-372

Plasmid

#210968

Purpose

Mammalian expression of full-length human TRIB1. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

TRIB1:M1-C372 (TRIB1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Puro-mScarlet-ACTB

Plasmid

#207754

Purpose

Donor template for Puro-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

ACTB Homology Arms flanking a Puro-mScarlet Cassette (ACTB Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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TagBFP-mEmerald-FRET

Plasmid

#58159

Purpose

Localization: Cytosolic FRET, Excitation: , Emission:

Depositor

Michael Davidson

Type

Empty backbone

Tags

TagBFP and mEmerald

Expression

Mammalian

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
UBH12 (3BI7)

Plasmid

#25219

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

ubh12.414.617 (UHRF1 Human)

Tags

His

Expression

Bacterial

Mutation

SRA Domain: Residues 414-617

Available Since

Aug. 12, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pEMS1613

Plasmid

#29278

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple141 (NR2E1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

Jan. 9, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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sfGFP-Zyxin-6

Plasmid

#56491

Purpose

Localization: Focal Adhesions, Excitation: 485, Emission: 507

Depositor

Michael Davidson

Insert

Zyxin (ZYX Human)

Tags

sfGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pZE-P9-egfp

Plasmid

#201790

Purpose

the promoter can be recognized by PadR

Depositor

Yajun Yan

Article

Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

Insert

P9

Expression

Bacterial

Mutation

WT

Available Since

July 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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mCardinal-NUP50-N-10

Plasmid

#56170

Purpose

Localization: Nuclear Pore, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

NUP50 (NUP50 Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
FusionRed-CD36-C-10

Plasmid

#56104

Purpose

Localization: Membrane, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

CD36 (CD36 Human)

Tags

FusionRed

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits