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pDONR221-SLC35A1_STOP

Plasmid

#161230

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35A1 (SLC35A1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Spike Display_Part 2 Spacer

Plasmid

#172730

Purpose

Encodes Part 2 of HexaPro-D614G spike to be used in the Spike Display system

Depositor

IIya Finkelstein

Article

Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

Insert

SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)

Use

Synthetic Biology

Mutation

Ectodomain only (AAs 1-1208); 682-685 (furin site…

Available Since

Aug. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pOpen-SapIM2

Plasmid

#165557

Purpose

Site-specific DNA methyltransferase 2; protects DNA from cleavage by SapI

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

M2.SapI

Use

Synthetic Biology

Available Since

Aug. 16, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pOpen-SapIM1

Plasmid

#165547

Purpose

Site-specific DNA methyltransferase 1; protects DNA from cleavage by SapI

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

M1.SapI

Use

Synthetic Biology

Available Since

Aug. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pOpen-SfiIM

Plasmid

#165538

Purpose

Site-specific DNA methyltransferase; protects DNA from cleavage by SfiI.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

M.SfiI

Use

Synthetic Biology

Available Since

Aug. 5, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pOpen-T4-BGT

Plasmid

#165530

Purpose

T4 Phage beta-glucosyltransferasetransfers the glucose moiety of UDP-Glc to the 5-hmC residues in double-stranded DNA.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

T4-BGT

Use

Synthetic Biology

Available Since

Aug. 5, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pOpen-HSlig4

Plasmid

#165503

Purpose

Ligase used in efficiently joining single-strand breaks in a double-strand polydeoxynucleotide in an ATP-dependent reaction.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

DNA Ligase IV

Use

Synthetic Biology

Available Since

July 27, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CBM9-ID-F

Plasmid

#171639

Purpose

Expresses SARS-CoV-2 Spike epitope

Depositor

Francis Nano

Article

McGuire et al Microb Cell Fact. 2022 Feb 5;21(1):21. d

Insert

SARS-CoV-2 Spike protein (S SARS-CoV-2)

Tags

CBM-9

Expression

Bacterial

Available Since

July 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CBM9-N

Plasmid

#171710

Purpose

Expresses SARS-CoV-2 Nucleocapsid protein epitope

Depositor

Francis Nano

Article

McGuire et al Microb Cell Fact. 2022 Feb 5;21(1):21. d

Insert

SARS-CoV-2 Nucleocapsid (N )

Tags

CBM-9

Expression

Bacterial

Available Since

July 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CBM9-ID-H1

Plasmid

#171709

Purpose

Expresses SARS-CoV-2 Spike epitope

Depositor

Francis Nano

Article

McGuire et al Microb Cell Fact. 2022 Feb 5;21(1):21. d

Insert

SARS-CoV-2 Spike protein (S SARS-CoV-2)

Tags

CBM-9

Expression

Bacterial

Available Since

July 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CBM9-(PT4)P

Plasmid

#171708

Purpose

Carries CBM9 fusion tag

Depositor

Francis Nano

Article

McGuire et al Microb Cell Fact. 2022 Feb 5;21(1):21. d

Insert

Cellulose Bindind Protein 9

Expression

Bacterial

Available Since

July 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLIC-lyn11-myc-LOV2GIVe (FA)

Plasmid

#170626

Purpose

For the mammalian expression of mLOV2GIVe GEF-deficient mutation F1685A

Depositor

Mikel Garcia-Marcos

Article

Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

Insert

Lyn11-myc-LOV2GIVe (FA)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-mas-GRK2RH-Nluc L118A

Plasmid

#158606

Purpose

Plasmid expressing GRK2(RH)-Nluc L118A in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain L118A - linker (GGTGGS) - Nluc

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-mas-KB-1753-Nluc I9A/W10A

Plasmid

#158604

Purpose

Plasmid expressing KB-1753-Nluc I9A/W10A in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 I9A/W10A - linker (GGTGGS) - Nluc

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC25A6_STOP

Plasmid

#161221

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A6 (SLC25A6 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC25A53_STOP

Plasmid

#161209

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A53 (SLC25A53 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC22A10_STOP

Plasmid

#161212

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC22A10 (SLC22A10 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC22A5_STOP

Plasmid

#161214

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC22A5 (SLC22A5 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC25A36_STOP

Plasmid

#161195

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A36 (SLC25A36 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC25A52_STOP

Plasmid

#161197

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A52 (SLC25A52 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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