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pGBW-m4134442

Plasmid

#152330

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp9

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4252962

Plasmid

#153660

Purpose

Mammalian expression plasmid for SARS-CoV-2 E (envelope)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133177

Plasmid

#153654

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp11

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp11 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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mVenus-Sequestosome1-N-18

Plasmid

#56335

Purpose

Localization: Membrane Scaffold, Excitation: 515, Emission: 528

Depositor

Michael Davidson

Insert

Sequestosome1 (SQSTM1 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits
SCAF4_pCSdest

Plasmid

#53797

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

SCAF4 (SCAF4 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pGBW-m4133655

Plasmid

#151978

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF8 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046831

Plasmid

#145671

Purpose

Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;Myc

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1624

Plasmid

#29280

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple153 (OXT Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

June 10, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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mMaroon-Cx43-7

Plasmid

#56198

Purpose

Localization: Gap Junctions, Excitation: 610, Emission: 652

Depositor

Michael Davidson

Insert

Cx43

Tags

mMaroon

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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tdEos-SSTR3-N-17

Plasmid

#57670

Purpose

Localization: Cilium, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

SSTR3

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp2-HF

Plasmid

#157684

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp2-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133801

Plasmid

#152974

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF7a protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF7a protein (ORF7a Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;S-tag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133508

Plasmid

#152957

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF3a protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133969

Plasmid

#152805

Purpose

Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;S-tag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4252851

Plasmid

#153806

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF6 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF6 protein (ORF6 Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134439

Plasmid

#152833

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp4

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4252852

Plasmid

#153644

Purpose

Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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mRuby2-Alpha-Actinin-19

Plasmid

#55890

Purpose

Localization: Alpha Actinin, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Alpha-Actinin (ACTN1 Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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4L1M

Plasmid

#60086

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

HERC2:YTC007-A11:C225804 (HERC2 Human)

Tags

6xHis

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pGBW-m4258309

Plasmid

#154807

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF6 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF6 protein (ORF6 Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;V5

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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