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pDEST-TDP-43-SUMO2-HA

Plasmid

#235495

Purpose

Expression of human TDP-43-SUMO2-HA fusion protein

Depositor

Maxime Rousseaux

Publication

Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

Insert

TARDBP (TARDBP Human)

Tags

HA

Expression

Mammalian

Available since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-cytoFLARE1.1-TEV

Plasmid

#234518

Purpose

Transcriptional reporter for detecting calcium transients in neuronal cultures (TEVp)

Depositor

Wenjing Wang

Publication

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

EGFP-P2A-CaM-V5-TEVp

Use

AAV

Expression

Mammalian

Available since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAG Lenti CMV EGFP-HA Puro

Plasmid

#236082

Purpose

Lentiviral backbone expressing C-terminally HA tagged EGFP, control for empty backbone 236080

Depositor

Melina Fan

Insert

EGFP

Use

Lentiviral

Tags

HA

Promoter

CMV

Available since

April 25, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5a1

Plasmid

#233462

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a1, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Publication

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a1, ColE1 ori, and KanR.

Use

CRISPR

Available since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5a2

Plasmid

#233463

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a2, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Publication

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a2, ColE1 ori, and KanR.

Use

CRISPR

Available since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5b1

Plasmid

#233465

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b1, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Publication

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b1, ColE1 ori, and KanR.

Use

CRISPR

Available since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5b2

Plasmid

#233466

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b2, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Publication

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b2, ColE1 ori, and KanR.

Use

CRISPR

Available since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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MERS-CoV Mpro strain HCoV-EMC 3CL protease domain

Plasmid

#228646

Purpose

Bacterial expression of the MERS-CoV Mpro domain for biochemical assay.

Depositor

ASAP Discovery, Frank von Delft

Insert

MERS-Cov Mpro domain

Tags

His-SUMO

Expression

Bacterial

Available since

March 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAG Retro EGFP Puro

Plasmid

#234463

Purpose

Control retroviral vector expressing Green Fluorescent Protein. Puromycin selection.

Depositor

Melina Fan, Addgene Research Program

Insert

Green Fluorescent Protein

Use

Retroviral

Available since

March 21, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAG Retro EGFP Hygro

Plasmid

#234462

Purpose

Control retroviral vector expressing Green Fluorescent Protein. Hygromycin selection.

Depositor

Melina Fan, Addgene Research Program

Insert

Green Fluorescent Protein

Use

Retroviral

Available since

March 21, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAG Retro Neo Empty

Plasmid

#234460

Purpose

Retroviral vector for cloning and expressing your gene of interest. Neomycin selection.

Depositor

Melina Fan, Addgene Research Program

Type

Empty backbone

Use

Retroviral

Available since

March 21, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAG Retro Hygro Empty

Plasmid

#234459

Purpose

Retroviral vector for cloning and expressing your gene of interest. Hygromycin selection.

Depositor

Melina Fan, Addgene Research Program

Type

Empty backbone

Use

Retroviral

Available since

March 21, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDF1b-iBOP

Plasmid

#229976

Purpose

Rhamnose inducible expression of Bambusa oldhamii PAL gene

Depositor

Christie Peebles

Publication

Hunstiger et al Biotechnol Prog. 2025 Mar-Apr;41(2):e351

Inserts

Prha Rhamnose Inducible Promoter
Bambusa oldhamii PAL

Expression

Bacterial

Promoter

Prha and n/a

Available since

March 11, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDF1b-iNTP

Plasmid

#229977

Purpose

Rhamnose inducible expression of Nicotiana tabacum PAL 4 gene

Depositor

Christie Peebles

Publication

Hunstiger et al Biotechnol Prog. 2025 Mar-Apr;41(2):e351

Inserts

Prha Rhamnose Inducible Promoter
Nicotiana tabacum PAL 4

Expression

Bacterial

Promoter

Prha and n/a

Available since

March 11, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDF1b-iRGP

Plasmid

#229978

Purpose

Rhamnose inducible expression of Rhodotorula glutinis RgPAL gene

Depositor

Christie Peebles

Publication

Hunstiger et al Biotechnol Prog. 2025 Mar-Apr;41(2):e351

Inserts

Prha Rhamnose Inducible Promoter
Rhodotorula glutinis RgPAL

Expression

Bacterial

Promoter

Prha and n/a

Available since

March 11, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAIP4_1AKZ

Plasmid

#234172

Purpose

Heterologous protein expression of uracil-DNA glycosylase (UDG) in Escherichia coli

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

1AKZ (UNG Human)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available since

March 10, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAIP4_3S60

Plasmid

#234231

Purpose

Heterologous protein expression of lectin OAA in Escherichia coli

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

3S60

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available since

March 7, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAIP4_6WEY

Plasmid

#234234

Purpose

Heterologous protein expression of replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) in Escherichia coli

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

6WEY (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAIP4_1YW5

Plasmid

#234235

Purpose

Heterologous protein expression of peptidyl-prolyl cis-trans isomerase in Escherichia coli

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

1YW5 (ESS1 Candida albicans)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAIP4_3W4Q

Plasmid

#234236

Purpose

Heterologous protein expression of beta-lactamase in Escherichia coli

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

3W4Q

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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