9,627 results
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Plasmid#207750PurposeDonor template for mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorInsertACTB Homology Arms flanking a mScarlet Tag (ACTB Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
EV-A71 2A protease; no tag, self cleaving
Plasmid#204813PurposeBacterial expresison of Enterovirus A71 2A proteaseDepositorInsertA71 2A protease
ExpressionBacterialAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Solo-sTagRFP-TUBA1B
Plasmid#207765PurposeDonor template for sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorInsertTUBA1B Homology Arms flanking a sTagRFP Tag (TUBA1B Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Blast-moxGFP-TUBA1B
Plasmid#207766PurposeDonor template for Blast-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorInsertTUBA1B Homology Arms flanking a Blast-moxGFP Cassette (TUBA1B Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-sTagRFP-Zeo
Plasmid#207734PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Zeo cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Zeo-sTagRFP
Plasmid#207701PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-sTagRFP cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-sTagRFP-Blast
Plasmid#207712PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Blast cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Puro-sTagRFP
Plasmid#207690PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-sTagRFP cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
ATAXIN3:1-361_BV
Plasmid#209732PurposeBacterial expression for structure determinationDepositorAvailable SinceNov. 13, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_EF-P49
Plasmid#202176PurposeLevel 0 plasmid containing a terminator sequence from gene 49202 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.DepositorInsertP49 terminator
UseSynthetic BiologyMutationNoneAvailable SinceOct. 19, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_DE-Per_sig_pep
Plasmid#202152PurposeLevel 0 plasmid containing a peroxisome signal peptide sequence with DE overhangs used to build level 1 constructs.DepositorInsertPeroxisome signal peptide
UseSynthetic BiologyMutationNoneAvailable SinceOct. 19, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKS2delARMec
Plasmid#130860PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseGateway entry vectorTagseGFP-FLAG-HAMutationDeletion of armadillo domainAvailable SinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKS2del4exp
Plasmid#130861PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
TagseGFP-FLAG-HAExpressionPlantMutationDeletion of terminal four amino acidsAvailable SinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKS2delKASHexp
Plasmid#130862PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
TagseGFP-FLAG-HAExpressionPlantMutationDeletion of KASH domainAvailable SinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKS2delTMexp
Plasmid#130863PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
TagseGFP-FLAG-HAExpressionPlantMutationDeletion of transmembrane domainAvailable SinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKS2delARMexp
Plasmid#130864PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
TagseGFP-FLAG-HAExpressionPlantMutationDeletion of armadillo domainAvailable SinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHWBF09exp
Plasmid#130833PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKP1
TagseGFP-FLAG-HAExpressionPlantPromoterCaMV 35SAvailable SinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKP2del4
Plasmid#130837PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKP2
TagseGFP-FLAG-HAExpressionPlantMutationDeletion of terminal four amino acid residuesPromoterCaMV 35SAvailable SinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKP2delKASH
Plasmid#130838PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKP2
TagseGFP-FLAG-HAExpressionPlantMutationDeletion of KASH domain, addition of three alaniā¦PromoterCaMV 35SAvailable SinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKP1delTM
Plasmid#130841PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKP1
TagseGFP-FLAG-HAExpressionPlantMutationDeletion of transmembrane domainPromoterCaMV 35SAvailable SinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits