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pRS314-PRP16
Plasmid#89971PurposePrp16 in a trp backbone complements prp16 genomic deletionDepositorInsertPRP16 (PRP16 Budding Yeast)
UseTagsExpressionYeastMutationnonePromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Prp16
Plasmid#111351Purposeprotein purificationDepositorInsertPRP16
UseTagsN-terminally 10xHis-tagged proteins with a Factor…ExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
HM110 prp16-2
Plasmid#111277Purposeprp16-2 in pSE358(Trp)DepositorInsertPRP16
UseTagsExpressionYeastMutationprp-2 (D575N)PromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB58 (Prp16)
Plasmid#111282PurposePrp16 in pSE358(Trp)DepositorInsertPRP16
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-PRP11
Plasmid#87594PurposeEncodes Y2H Gal4 DNA binding domain for Prp11DepositorInsertPRP11 (PRP11 Budding Yeast)
UseTagsGAL4 BD and MycExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
hPrp16 N-terminus
Plasmid#11204DepositorInsertPrp16 N-terminus (DHX38 Human)
UseTagsGSTExpressionBacterialMutationAmino acids 1-554PromoterAvailable sinceJan. 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
hPrp16 C-terminus
Plasmid#11205DepositorInsertPrp16 C-terminus (DHX38 Human)
UseTagsGSTExpressionBacterialMutationAmino acids 948-1227PromoterAvailable sinceJan. 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRS314-prp16-1
Plasmid#89972Purposeprp16-1 allele in TRP plasmidDepositorInsertPRP16 (PRP16 Budding Yeast)
UseTagsExpressionYeastMutationprp16-1 allele (Y386D)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-Prp18
Plasmid#111354Purposeprotein purificationDepositorInsertPRP18
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS314-prp16-302
Plasmid#89974Purposeprp16-302 allele in TRP plasmidDepositorInsertPRP16 (PRP16 Budding Yeast)
UseTagsExpressionYeastMutationprp16-302 allele (R456K and G691R)PromoterAvailable sinceAug. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS314-prp16-101
Plasmid#89973Purposeprp16-101 allele in TRP plasmidDepositorInsertPRP16 (PRP16 Budding Yeast)
UseTagsExpressionYeastMutationprp16-101 allele (G373S)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(WT)
Plasmid#111323Purposefor T7 transcription of wt actin pre-mRNADepositorInsertACT1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(del6) AcAc
Plasmid#111332PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutation3' splice site AcAcPromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(WTdel6)
Plasmid#111324Purposefor T7 transcription of wt actin pre-mRNA (del6)DepositorInsertACT1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(AcAc)
Plasmid#111327PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutation3' splice site AcAc mutation in intronPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(BrG)
Plasmid#111326PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutationbranch site A to G mutation in intronPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(BrC)
Plasmid#111325PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutationbranch site A to C mutation in intronPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(del6) A259C
Plasmid#111329PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutationbranch site A259CPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(del6) C256A
Plasmid#111331PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutationbranch site C256APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(del6) short BP-3'SS
Plasmid#111333PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutationshorter branch to 3' splice site distancePromoterAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
TAL3519
Plasmid#47238DepositorInsertZebrafishCommunity-prp19-right
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
TAL3518
Plasmid#47237DepositorInsertZebrafishCommunity-prp19-left
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pGEM4Z-OMA1
Plasmid#61718Purposehuman Oma1 protease in the E.coli/in vitro translation vectorDepositorInsertOMA1 (OMA1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4-OMA1
Plasmid#61719Purposemammalian expression of human Oma1 with C-terminal FLAG tagDepositorInsertOMA1 (OMA1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
4LG8
Plasmid#60095PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertPRPF19:JMC069-A01:C226630 (PRPF19 Human)
UseBaculovirus expressionTags6xHisExpressionMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
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N-GFP-Dcp2
Plasmid#163585Purposeexpress N-terminal GFP tagged Dcp2 FL at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationPromoterDCP2 promoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 ΔH1 WD
Plasmid#163580Purposeexpress Dcp2 ΔH1 WD at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationW50A, D54A, L255APromoterDCP2 promoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 ΔH1
Plasmid#163576Purposeexpress Dcp2 ΔH1 (L255A) at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationLysine at position 255 is mutated to AlaninePromoterDCP2 promoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 FL-H1
Plasmid#163583Purposeexpress Dcp2 FL-H1 at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationThe first HLM (EDQLKSYAEEQLKLLLGITKEE) is added t…PromoterDCP2 promoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 ΔN
Plasmid#163575Purposeexpress wild type Dcp2 ΔN(243-970) at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationTruncation of N-terminal domain (1-242) of Dcp2PromoterDCP2 promoterAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 300
Plasmid#163577Purposeexpress Dcp2 300 at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationTruncation of C-terminal domian (301-970)PromoterDCP2 promoterAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2C Δ5H-H1
Plasmid#163584Purposeexpress Dcp2 FL-H1 at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationTruncation of aa1-326; the first HLM (EDQLKSYAEEQ…PromoterDCP2 promoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 300 AAAA
Plasmid#163579Purposeexpress Dcp2 300 AAAA at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationTruncation of C-terminal domain (301-970); mutati…PromoterDCP2 promoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 300 ΔH1
Plasmid#163578Purposeexpress Dcp2 300 ΔH1 at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationTruncation of C-terminal domain (301-970); Lysine…PromoterDCP2 promoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 ΔH1 Δ5H
Plasmid#163582Purposeexpress Dcp2 ΔH1 Δ5H at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationL255A; mutate 440-450, 489-500, 701-708, 827-831,…PromoterDCP2 promoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2C Δ5H
Plasmid#163581Purposeexpress Dcp2C Δ5H at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationTruncation of aa1-300; mutate aa440-450, aa489-50…PromoterDCP2 promoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1 LIC 6A HIS-TEV-DPP8
Plasmid#166793PurposeMammalian cell expression vector for Flag-tagged DPP8DepositorInsertDPP8 (DPP8 Human)
UseTagsHIS-TEVExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A FLAG-TEV-DPP8
Plasmid#166795PurposeMammalian cell expression vector for FLAG-tagged DPP8DepositorInsertDPP8 (DPP8 Human)
UseTagsFLAG-TEVExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_K700E_hSF3B1
Plasmid#176591PurposeAdenoviral construct to change K to E at 700th amino acid (K700E) in human SF3B1DepositorInsertSF3B1 (SF3B1 Human)
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_FLAG_hSF3B1
Plasmid#176590PurposeAdenoviral construct to insert a N-terminal FLAG to one allele of human SF3B1DepositorInsertSF3B1 (SF3B1 Human)
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Mfn2-HA
Plasmid#139192PurposeExpresses C-terminal HA tagged wt human Mfn2 in mammalian cellsDepositorInsertMFN2 (MFN2 Human)
UseTags3HAExpressionMammalianMutationPromoterCMV promoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-FLAG-SF3B1-WT
Plasmid#82576PurposeExpresses a codon-optimized ORF of human SF3B1 (wild-type)DepositorInserthuman SF3B1-WT (SF3B1 Human)
UseTagsFLAG peptideExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
plko-shmfn2-mfn2-Rescue
Plasmid#160655Purpose"Rescue mfn2 expression by adding the guide-resistant-mutant mfn2 sequence"DepositorInsertmfn2 (MFN2 Human)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only
