We narrowed to 84 results for: 44249

1. pdCas9-bacteria

   Plasmid

   #44249

   Purpose

   aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dCas9 (bacteria)
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   pLtetO-1

   Available Since

   April 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pHdCas9

   Plasmid

   #137080

   Purpose

   Expression of Himar-dCas9 in bacteria

   Depositor

   Harris Wang

   Article

   Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

   Insert

   Himar-dCas9
   

   Expression

   Bacterial

   Promoter

   pLtetO-1

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLY108

   Plasmid

   #130938

   Purpose

   Engineered sgRNA-LEB1 (2 BoxB aptamers) generator circuit including promoter Plux2

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-LEB1
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Plux2

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLY103

   Plasmid

   #130937

   Purpose

   Engineered sgRNA-LEA1 (2 BoxB aptamers) generator circuit including promoter Plux2

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-LEA1
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Plux2

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLY76

   Plasmid

   #130963

   Purpose

   Engineered sgRNA-LEA2 (2 BoxB aptamers) generator circuit including promoter Plux2

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-LEA2
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Plux2

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLY74

   Plasmid

   #130945

   Purpose

   Engineered sgRNA-G6 (2 BoxB aptamers) generator circuit including promoter Plux2

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-G6
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Plux2

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLY111

   Plasmid

   #130942

   Purpose

   Engineered sgRNA-LEB4 (2 BoxB aptamers) generator circuit including promoter Plux2

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-LEB4
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Plux2

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLY110

   Plasmid

   #130940

   Purpose

   Engineered sgRNA-LEB3 (2 BoxB aptamers) generator circuit including promoter Plux2

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-LEB3
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Plux2

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLY77

   Plasmid

   #130964

   Purpose

   Engineered sgRNA-LEB2 (2 BoxB aptamers) generator circuit including promoter Plux2

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-LEB2
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Plux2

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLY124

   Plasmid

   #130946

   Purpose

   Engineered sgRNA-G6-2MS2 (2 MS2 aptamers) generator circuit including promoter Pbad

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-G6-2MS2
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Pbad

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLY112

    Plasmid

    #130944

    Purpose

    Engineered sgRNA-LEB5 (2 BoxB aptamers) generator circuit including promoter Plux2

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEB5
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Plux2

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLY107

    Plasmid

    #130943

    Purpose

    Engineered sgRNA-LEA5 (2 BoxB aptamers) generator circuit including promoter Plux2

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEA5
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Plux2

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLY105

    Plasmid

    #130939

    Purpose

    Engineered sgRNA-LEA3 (2 BoxB aptamers) generator circuit including promoter Plux2

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEA3
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Plux2

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLY106

    Plasmid

    #130941

    Purpose

    Engineered sgRNA-LEA4 (2 BoxB aptamers) generator circuit including promoter Plux2

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEA4
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Plux2

    Available Since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. dCas9 fused to nanobody (anti-beta catenin)

    Plasmid

    #186420

    Purpose

    Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

    Depositor

    Stephen Elledge

    Article

    Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

    Insert

    dCas9-nanobody fusion (anti-beta catenin)
    

    Use

    CRISPR

    Tags

    6His

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pdCas9-omega

    Plasmid

    #114632

    Purpose

    Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

    Depositor

    Anushree Chatterjee

    Article

    Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

    Insert

    dCas9-omega
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Tet promoter

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLY86

    Plasmid

    #130949

    Purpose

    Engineered sgRNA-LEA2-WTmis2 generator circuit including promoter Plux2, 2 BoxB aptamers and 2 mis-matches in sgRNA scaffold

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEA2-WTmis2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Plux2

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLY185

    Plasmid

    #184146

    Purpose

    RFP generator controlled by Plux2 promoter

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    rfp
    

    Use

    Synthetic Biology

    Promoter

    Plux2

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. dCas9 fused to scFv (anti-spike)

    Plasmid

    #186421

    Purpose

    Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

    Depositor

    Stephen Elledge

    Article

    Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

    Insert

    dCas9-scFv fusion (anti-SARS-CoV-2 spike)
    

    Use

    CRISPR

    Tags

    6His

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLY227

    Plasmid

    #184148

    Purpose

    short crRNA generator with spacer LEA2 with WT repeat region

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    s-crRNA-LEA2-WT
    

    Use

    Synthetic Biology

    Promoter

    Plux2

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge