3,636 results

1. pAc-y1sgRNA-Cas9

   Plasmid

   #49331

   Purpose

   Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   Ji-Long Liu

   Article

   Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

   Inserts

   Cas9
   dU6-sgRNA
   y1 sgRNA (y Fly)
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Insect

   Mutation

   Human codon optimised

   Promoter

   Actin-5c and Drosophila U6

   Available Since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFOSGE_COF4C01

   Plasmid

   #71009

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Cand1 (CG5366 Fly)
   

   Use

   Gateway entry vector

   Available Since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pBac{3XP3::EGFP, Pactin::Ptrsps}

   Plasmid

   #86861

   Purpose

   Expresses EGFP in eyes and ubiquitous expression of P-transposase

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

   Insert

   pACNNTNPII-S129A
   

   Expression

   Insect

   Available Since

   June 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)

   Plasmid

   #172066

   Purpose

   Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intron

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   mTagBFP2(11x7)
   

   Expression

   Insect

   Available Since

   Oct. 20, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. K560_AviC_coBirA

   Plasmid

   #129765

   Purpose

   Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Tags

   Avi-tag GLNDIFEAQKIEWH and His6

   Expression

   Bacterial

   Mutation

   Truncation at 559

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB1H2w2-mutOdd

   Plasmid

   #18044

   Depositor

   Scot Wolfe

   Article

   Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

   Insert

   mutant Odd Skipped
   

   Expression

   Bacterial

   Mutation

   A mutant version of the zinc finger protein Odd S…

   Available Since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ftz-M3

   Plasmid

   #11245

   Depositor

   Robin Reed

   Article

   Zhou et al Proc Natl Acad Sci U S A. 2002 Sep 17. 9

   Insert

   Ftz-M3
   

   Tags

   3 MS2 hairpins

   Expression

   Bacterial

   Available Since

   Jan. 19, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pBAC-DsRed-QF2-hsp70

   Plasmid

   #104876

   Purpose

   General purpose vector for cloning enhancer/promoter regions to drive QF2 expression; used for mosquito

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

   Insert

   QF2
   

   Expression

   Insect

   Available Since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUAST-attB-3xHA-V5-RpL10Ab

   Plasmid

   #125223

   Purpose

   3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression

   Depositor

   Herman Dierick

   Article

   Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

   Insert

   RpL10Ab (RpL10Ab Fly)
   

   Tags

   3x HA, V5

   Expression

   Insect

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJFRC-20X-UAS-ADARcd

    Plasmid

    #81173

    Purpose

    identification of cell-specific targets of RNA binding proteins

    Depositor

    Michael Rosbash

    Article

    McMahon et al Cell. 2016 Mar 30. pii: S0092-8674(16)30

    Insert

    UAS-ADARcd
    

    Expression

    Insect

    Promoter

    UAS

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-6p-1_GST-DmKHC[1-421]_1xmNeonGreen

    Plasmid

    #196974

    Purpose

    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal mNeonGreen tag and cleavable N-terminal GST tag

    Depositor

    Lukas Kapitein

    Article

    Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Tags

    GST and mNeonGreen

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT-mCherry-Patronin

    Plasmid

    #31753

    Depositor

    Ron Vale

    Article

    Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

    Insert

    Patronin (Patronin Fly)
    

    Tags

    mCherry

    Expression

    Insect

    Promoter

    Metallothionein

    Available Since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

13. OA-1043

    Plasmid

    #164586

    Purpose

    clone in U6 and DR

    Depositor

    Omar Akbari

    Article

    Sun et al J Vis Exp. 2021 Feb 5;(168). doi: 10.379

    Insert

    U6 and DR
    

    Expression

    Insect

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. dmBACCS2-IRES-dOrai-IRES-GFP

    Plasmid

    #72893

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai-IRES-GFP
    

    Tags

    IRES-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUAS-ERK-YFP

    Plasmid

    #42076

    Depositor

    Norbert Perrimon

    Article

    Friedman et al Nature. 2006 Nov 9;444(7116):230-4. Epub

    Insert

    Drosophila ERK ortholog (rl Fly)
    

    Tags

    YFP

    Expression

    Insect

    Promoter

    Hsp70

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pDmVasa

    Plasmid

    #165783

    Purpose

    Drosohphila vasa germline promoter and 5'UTR

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Vasa promoter
    

    Expression

    Insect

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDropIn-T2A-split-intein-Gal4[1-20]-N-int

    Plasmid

    #199195

    Purpose

    Insert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed
    

    Expression

    Insect

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDropIn-T2A-split-intein-Gal4[21-881]-C-int

    Plasmid

    #199196

    Purpose

    Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed
    

    Expression

    Insect

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZG61

    Plasmid

    #202575

    Purpose

    RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-35S-hptII

    Depositor

    Guoyong Xu

    Article

    Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

    Insert

    HyperADARcd
    

    Expression

    Plant

    Mutation

    E488Q

    Available Since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. 10xUAS-GFP-DD

    Plasmid

    #104989

    Purpose

    Expresses GFP fused with destabilizing domain (DD) downstream of a 10XUAS sequence.

    Depositor

    Jing-W Wang

    Article

    Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

    Insert

    TMP-inducible destabilized GFP (GFP-DD)
    

    Tags

    DD

    Expression

    Insect

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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