3,636 results

1. pBAC-DsRed-QF2-hsp70

   Plasmid

   #104876

   Purpose

   General purpose vector for cloning enhancer/promoter regions to drive QF2 expression; used for mosquito

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

   Insert

   QF2
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. K560_AviC_coBirA

   Plasmid

   #129765

   Purpose

   Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Use

   Tags

   Avi-tag GLNDIFEAQKIEWH and His6

   Expression

   Bacterial

   Mutation

   Truncation at 559

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZG61

   Plasmid

   #202575

   Purpose

   RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-35S-hptII

   Depositor

   Guoyong Xu

   Article

   Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

   Insert

   HyperADARcd
   

   Use

   Tags

   Expression

   Plant

   Mutation

   E488Q

   Promoter

   Available since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc-y1sgRNA-Cas9

   Plasmid

   #49331

   Purpose

   Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   Ji-Long Liu

   Article

   Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

   Inserts

   Cas9
   dU6-sgRNA
   y1 sgRNA (y Fly)
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Insect

   Mutation

   Human codon optimised

   Promoter

   Actin-5c and Drosophila U6

   Available since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUAS-ERK-YFP

   Plasmid

   #42076

   Depositor

   Norbert Perrimon

   Article

   Friedman et al Nature. 2006 Nov 9;444(7116):230-4. Epub

   Insert

   Drosophila ERK ortholog (rl Fly)
   

   Use

   Tags

   YFP

   Expression

   Insect

   Mutation

   Promoter

   Hsp70

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pAc5.1B-EGFP-DmAtx2_AE

   Plasmid

   #148548

   Purpose

   Insect Expression of DmAtx2

   Depositor

   Elisa Izaurralde

   Insert

   DmAtx2 (Atx2 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Three silent mutations and two deletions compared…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 10xUAS-GFP-DD

   Plasmid

   #104989

   Purpose

   Expresses GFP fused with destabilizing domain (DD) downstream of a 10XUAS sequence.

   Depositor

   Jing-W Wang

   Article

   Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

   Insert

   TMP-inducible destabilized GFP (GFP-DD)
   

   Use

   Tags

   DD

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUWR-CadTS-C

   Plasmid

   #58326

   Purpose

   Destination vector for inserting ubi-CadTS control to Drosophila melanogaster genome

   Depositor

   Denise Montell

   Article

   Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

   Insert

   E-cadherin tension sensor control
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   poly-ubiquitin

   Available since

   Aug. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pACUH-sfcherry2(1-10)

   Plasmid

   #172061

   Purpose

   UAS vector to express sfcherry2(1-10) in Drosophila

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   sfcherry2(1-10)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pB1H2w2-mutOdd

    Plasmid

    #18044

    Depositor

    Scot Wolfe

    Article

    Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

    Insert

    mutant Odd Skipped
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A mutant version of the zinc finger protein Odd S…

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJFRC7-syt1-GCaMP6f

    Plasmid

    #190896

    Purpose

    GCaMP6f fused to Drosophila synaptotagmin1 via a 3x GS linker. In the pJFRC7-20XUAS vector.

    Depositor

    Tom Clandinin

    Article

    Turner et al Elife. 2022 Oct 27;11. pii: 82587. doi:

    Insert

    syt1-GCaMP6f
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJFRC-20X-UAS-ADARcd

    Plasmid

    #81173

    Purpose

    identification of cell-specific targets of RNA binding proteins

    Depositor

    Michael Rosbash

    Article

    McMahon et al Cell. 2016 Mar 30. pii: S0092-8674(16)30

    Insert

    UAS-ADARcd
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRmHA3-CH2F2-CG9866

    Plasmid

    #52286

    Purpose

    Expresses Drosophila Ataxin-7

    Depositor

    Jerry Workman

    Article

    Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

    Insert

    Ataxin-7 (CG9866 Fly)
    

    Use

    Tags

    2xHA 2xFLAG

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. dmBACCS2-IRES-dOrai-IRES-GFP

    Plasmid

    #72893

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai-IRES-GFP
    

    Use

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAc5.1B-EGFP-DmFMR1_B

    Plasmid

    #145920

    Purpose

    Insect Expression of DmFMR1

    Depositor

    Elisa Izaurralde

    Insert

    DmFMR1 (Fmr1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Two amino acid mutations (T214I and N501S) and th…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. WT (DVD)

    Plasmid

    #184316

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMT-TAK1-V5-His

    Plasmid

    #63753

    Purpose

    Expresses drosophila TAK1 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

    Depositor

    Cyril Gueydan

    Insert

    TGF-β activated kinase 1 (Tak1 Fly)
    

    Use

    Tags

    V5-His

    Expression

    Insect

    Mutation

    Promoter

    pMT

    Available since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. dmBACCS2NS-IRES-dOrai-IRES-GFP

    Plasmid

    #72895

    Purpose

    Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2NS-IRES-dOrai-IRES-GFP
    

    Use

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUASTLOTattB_eGFP::Dpp

    Plasmid

    #163702

    Purpose

    GFP-tagged version of Dpp (Decapentaplegic) under control of UAS and LexO enhancers

    Depositor

    Markus Affolter

    Article

    Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. do

    Insert

    decapentaplegic (dpp Fly)
    

    Use

    Cre/Lox

    Tags

    Tagged with eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. RP125 pUAST EGFP:Orco

    Plasmid

    #53441

    Purpose

    N-terminal fusion of EGFP to Drosophila melanogaster Orco

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    EGFP:Orco (Orco Fly)
    

    Use

    Tags

    EGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only