Skip to main content
Addgene
Showing: 451 - 478 of 478 results

  9. pcDNA3.1-EF1A-mNeonGreen2x-SREBP1-P2A-Puro

    Plasmid
    #228401
    Purpose
    Encodes a N-terminal truncated version of SREBP1 lacking part of the transcriptional activation domain and fused to a tandem of mNeongreen to visualize the import of SREBP1
    Depositor
    Dirk Daelemans
    Article
    Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:
    Insert
    SREBP1 (SREBF1 Human)
    Use
    Tags
    mNeonGreen2x
    Expression
    Mammalian
    Mutation
    deleted N terminal activation domain (amino acids…
    Promoter
    EF1a
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    228401-plasmid-map-sequence-id-457257

    Enlarge

  10. TFORF2863

    Plasmid
    #143646
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SREBF1 (SREBF1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143646-plasmid-map-sequence-id-400462

    Enlarge

  11. TFORF2862

    Plasmid
    #143645
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SREBF1 (SREBF1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143645-plasmid-map-sequence-id-400473

    Enlarge

  12. pCDNA3-SREBP-1 S1P and S2P mutations (U124AFG200-5)

    Plasmid
    #210767
    Purpose
    Mammalian expression of SREBP-1 (isoform 1) with mutations in residues reported to be critical for S1P and S2P cleavage.
    Depositor
    Allan Weissman
    Article
    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.
    Insert
    SREBP-1 (SREBF1 Human)
    Use
    Tags
    E-tag and T7x2
    Expression
    Mammalian
    Mutation
    DRSR 514-517 to AS; 557 R to A; multiple silent m…
    Promoter
    Available since
    April 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    210767-plasmid-map-sequence-id-421642

    Enlarge

  21. AAP-COMP-blac-flag-his

    Plasmid
    #111001
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    aminopeptidase P (APP) (PF14_0517 Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111001-plasmid-map-sequence-id-224546

    Enlarge

  24. pNICBio2_WDR5:2-334

    Plasmid
    #211027
    Purpose
    bacterial expression of human WDR5 fragment A2 to C334. N-terminal 10X His tag with TEV cleavage and C-terminal biotin acceptor peptide tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    WDR5:A2-C334 (WDR5 Human)
    Use
    Tags
    MHHHHHHHHHHDLGTENLYFQS and SSKGGYGLNDIFEAQKIEWHE
    Expression
    Bacterial
    Mutation
    wild type
    Promoter
    T7
    Available since
    April 4, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    211027-plasmid-map-sequence-id-426277

    Enlarge

Showing: 451 - 478 of 478 results