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Showing: 451 - 500 of 12522 results

  1. Click editor utilizing Cdc13 for template recruitment - pCMV-T7-Cdc13-nCas9-EcKlenow (JO1293)

    Plasmid
    #217796
    Purpose
    Variant CE1 construct with Cdc13 telomere binding protein for template recruitment, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Cdc13-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217796-plasmid-map-sequence-id-432863

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  4. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

    Plasmid
    #217808
    Purpose
    Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217808-plasmid-map-sequence-id-432916

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  7. Click editor utilizing Cdc13(Y556A) for template recruitment - pCMV-T7-Cdc13(Y556A)-nCas9-EcKlenow (JO1329)

    Plasmid
    #217797
    Purpose
    Variant CE1 construct with Cdc13(Y556A) telomere binding protein for template recruitment, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Cdc13(Y556A)-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Cdc13(Y556A); nSpCas9(H840A); EcKlenow(-exo;D355A…
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217797-plasmid-map-sequence-id-432864

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  8. Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)

    Plasmid
    #217803
    Purpose
    Variant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    mSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217803-plasmid-map-sequence-id-432890

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  9. SPOCK2_22-424_AviN_HisC

    Plasmid
    #195877
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SPOCK2 (SPOCK2 Human)
    Use
    Pfhmsp-avin-lic-c, baculovirus expression
    Tags
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195877-plasmid-map-sequence-id-387267

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  10. NXPH1_22-271_AviN_HisC

    Plasmid
    #195872
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NXPH1 (NXPH1 Human)
    Use
    Pfhmsp-avin-lic-c, baculovirus expression
    Tags
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195872-plasmid-map-sequence-id-387247

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  11. SPOCK1_23-439_HisN_AviC

    Plasmid
    #195876
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SPOCK1 (SPOCK1 Human)
    Use
    Pfhmsp-avic-lic-n, baculovirus expression
    Tags
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195876-plasmid-map-sequence-id-387253

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  12. OLFML3_123-406_AviN_HisC

    Plasmid
    #195871
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    OLFML3 (OLFML3 Human)
    Use
    Pfhmsp-avin-lic-c, baculovirus expression
    Tags
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195871-plasmid-map-sequence-id-397083

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  13. NTN1_39-453_AviN_HisC

    Plasmid
    #195873
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NTN1 (NTN1 Human)
    Use
    Pfhmsp-avin-lic-c, baculovirus expression
    Tags
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195873-plasmid-map-sequence-id-387265

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  14. COL11A1_36-250_HisN_AviC

    Plasmid
    #195878
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    COL11A1 (COL11A1 Human)
    Use
    Pfhmsp-avic-lic-n, baculovirus expression
    Tags
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195878-plasmid-map-sequence-id-387254

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  15. COL11A1_1570-1806_HisN_AviC

    Plasmid
    #195879
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    COL11A1 (COL11A1 Human)
    Use
    Pfhmsp-avic-lic-n, baculovirus expression
    Tags
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195879-plasmid-map-sequence-id-387249

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  16. ECE1_101-770_HisN_AviC

    Plasmid
    #195875
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ECE1 (ECE1 Human)
    Use
    Pfhmsp-avic-lic-n, baculovirus expression
    Tags
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195875-plasmid-map-sequence-id-387251

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  17. COL25A1_53-654_AviN_HisC

    Plasmid
    #195880
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    COL25A1 (COL25A1 Human)
    Use
    Pfhmsp-avin-lic-c, baculovirus expression
    Tags
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195880-plasmid-map-sequence-id-387264

    Enlarge

  18. 8VIS

    Plasmid
    #220881
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TMPRSS11D (TMPRSS11D Human)
    Use
    Baculovirus expresssion
    Tags
    EFVEHHHHHHHH
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    Sept. 30, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220881-plasmid-map-sequence-id-436700

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  19. avi-eTMPRSS11D S368A

    Plasmid
    #220882
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TMPRSS11D (TMPRSS11D Human)
    Use
    Baculovirus expresssion
    Tags
    Honeybee melittinsignal
    Expression
    Mutation
    S368A
    Promoter
    Polyhedrin
    Available since
    June 20, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220882-plasmid-map-sequence-id-436699

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  20. 8V04

    Plasmid
    #220879
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TMPRSS2 (TMPRSS2 Human)
    Use
    Baculovirus expresssion
    Tags
    EFVEHHHHHHHH and Honeybee melittin signal
    Expression
    Mutation
    S251D/R252D/Q253D/S254D/R255K
    Promoter
    Polyhedrin
    Available since
    Sept. 30, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220879-plasmid-map-sequence-id-436702

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  22. 3D8H

    Plasmid
    #51332
    Depositor
    Cheryl Arrowsmith
    Insert
    3D8H
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    51332-plasmid-map-sequence-id-338743

    Enlarge

  23. 3LMK

    Plasmid
    #51339
    Depositor
    Cheryl Arrowsmith
    Insert
    3LMK
    Use
    Baculovirus expression
    Tags
    His
    Expression
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 24, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. 3DAX

    Plasmid
    #51333
    Depositor
    Cheryl Arrowsmith
    Insert
    3DAX
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 24, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. 2I81

    Plasmid
    #51352
    Depositor
    Cheryl Arrowsmith
    Insert
    2I81
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. 2PSW

    Plasmid
    #51342
    Depositor
    Cheryl Arrowsmith
    Insert
    2PSW
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  27. 3CKL

    Plasmid
    #51359
    Depositor
    Cheryl Arrowsmith
    Insert
    3CKL
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. 4IDI

    Plasmid
    #51321
    Depositor
    Cheryl Arrowsmith
    Insert
    4IDI
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  29. 2NQ3

    Plasmid
    #51341
    Depositor
    Cheryl Arrowsmith
    Insert
    2NQ3
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  30. 2P5S

    Plasmid
    #51354
    Depositor
    Cheryl Arrowsmith
    Insert
    2P5S
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. 2PMY

    Plasmid
    #51355
    Depositor
    Cheryl Arrowsmith
    Insert
    2PMY
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  32. ZIKV_KX377337.1_NS2B

    Plasmid
    #234404
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    NS2B_ZIKV
    Use
    Tags
    MGSSHHHHHHSSGLVPRGS
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234404-plasmid-map-sequence-id-470068

    Enlarge

  33. Denv2_GU289914.1_NS2B

    Plasmid
    #234401
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    NS2B_DENV2
    Use
    Tags
    MGSSHHHHHHSSGLVPRGS
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234401-plasmid-map-sequence-id-470061

    Enlarge

  34. CHICKV_KY575571.1_NSP2_Hel_NAviCHis

    Plasmid
    #234397
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    Nsp2_CHICKV
    Use
    Tags
    MHHHHHHSSGVDLG and SSKGGYGLNDIFEAQKIEWHE
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    234397-plasmid-map-sequence-id-469869

    Enlarge

  35. Denv4_KJ160504.1_NS2B

    Plasmid
    #234402
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    NS2B_DENV4
    Use
    Tags
    MGSSHHHHHHSSGLVPRGS
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    234402-plasmid-map-sequence-id-469864

    Enlarge

  36. 2NQA

    Plasmid
    #51330
    Depositor
    Cheryl Arrowsmith
    Insert
    2NQA
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  37. RIPK2A-c033

    Plasmid
    #53642
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RIPK2A
    Use
    Baculovirus expression
    Tags
    His6-TEV
    Expression
    Mutation
    R171C
    Promoter
    polyhedrin
    Available since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  38. DDR1A-c002

    Plasmid
    #53687
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DDR1A
    Use
    Baculovirus expression
    Tags
    His6-TEV
    Expression
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    June 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  39. WestNileVirus_NC_009942_NS2B

    Plasmid
    #234403
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    NS2B_WestNileVirus
    Use
    Tags
    MGSSHHHHHHSSGLVPRGS
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234403-plasmid-map-sequence-id-470064

    Enlarge

  40. UTYC-c613

    Plasmid
    #53685
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    UTYC
    Use
    Baculovirus expression
    Tags
    His6-TEV
    Expression
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    July 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  41. YANK1A-c051

    Plasmid
    #53680
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    YANK1A
    Use
    Baculovirus expression
    Tags
    His6-TEV
    Expression
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    July 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  42. PsuDJ-1.1

    Plasmid
    #52080
    Purpose
    Encodes Panagrolaimus superbus DJ-1. Potent antioxidant and molecular chaperone
    Depositor
    Ann Burnell
    Insert
    PsuDJ-1.1
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 4, 2014
    Availability
    Academic Institutions and Nonprofits only

  43. ACVR1A-c076

    Plasmid
    #53691
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACVR1A
    Use
    Baculovirus expression
    Tags
    His6-TEV
    Expression
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    June 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  44. CCNKA-c001

    Plasmid
    #53644
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CCNKA
    Use
    Baculovirus expression
    Tags
    His6-TEV
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  45. KCNK10C-c011

    Plasmid
    #53639
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KCNK10C
    Use
    Baculovirus expression
    Tags
    TEV-His10-Flag
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    July 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  46. BMPR1BA-c017

    Plasmid
    #53690
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BMPR1BA
    Use
    Baculovirus expression
    Tags
    His6-TEV
    Expression
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    June 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  47. 3C8X

    Plasmid
    #51336
    Depositor
    Cheryl Arrowsmith
    Insert
    3C8X
    Use
    Baculovirus expression
    Tags
    His
    Expression
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 24, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  48. ABCB10A-c109

    Plasmid
    #53676
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ABCB10A
    Use
    Baculovirus expression
    Tags
    His6-TEV
    Expression
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  49. GBE1A-c106

    Plasmid
    #53640
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GBE1A
    Use
    Baculovirus expression
    Tags
    His6-TEV
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  50. DCLRE1BA-c026

    Plasmid
    #53645
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DCLRE1BA
    Use
    Baculovirus expression
    Tags
    His6-TEV
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 451 - 500 of 12522 results