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pEGFP-N1-mSNX27 RNAi-resistant

Plasmid

#163620

Purpose

mouse SNX27 cDNA with point mutation to resist siRNA against SNX27 C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27 RNAi-resistant (Snx27 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

RNAi resistance

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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OSCA1.2-K435L-L438K-peGFP-N1

Plasmid

#228587

Purpose

Expresses OSCA1.2-K435L-L438K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

K435L, L438K

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-PARP1-delM43;F44I

Plasmid

#211579

Purpose

CMV driven PARP1-delM43;F44I -eGFP expressing construct with IRES-Neomycin selection

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Contains deletion of M43 and F44I

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 D406A M407A

Plasmid

#112107

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D406A M407A

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 D406R M407R

Plasmid

#112109

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D406R M407R

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Full length Importin beta

Plasmid

#106941

Purpose

Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

Depositor

Patrizia Lavia

Article

Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

Insert

human Importin beta-1 (KPNB1) (KPNB1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-human lyn(C468A)–GFP

Plasmid

#35959

Purpose

Expresses human lyn protein codon optimized for zebrafish expression.

Depositor

Anna Huttenlocher

Article

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

LYN (LYN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

c468a

Promoter

cmv

Available since

April 27, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-CaMKKbeta S129D, S133D, S137D

Plasmid

#32455

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

S129D, S133D, S137D

Promoter

CMV

Available since

May 31, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-CaMKKbeta S129A, S133A, S137A

Plasmid

#32454

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

S129A, S133A, S137A

Promoter

CMV

Available since

April 23, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-zebrafish lyn(C466A)–GFP

Plasmid

#35957

Depositor

Anna Huttenlocher

Article

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

humanized zebrafish lyn (lyn Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

C466A

Promoter

cmv

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mSNX27-H112A RNAi-resistant

Plasmid

#163621

Purpose

mouse SNX27 cDNA with point mutation to resist siRNA against SNX27 and with a point mutation H112A C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27-H112A RNAi-resistant (Snx27 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

H112A and RNAi resistance

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 R290A D291A T292A

Plasmid

#112103

Purpose

Mammalian expression of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

R290A D291A T292A

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 T292A L293A P294A

Plasmid

#112104

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T292A L293A P294A

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 L330R Q334R L702R

Plasmid

#112105

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

L330R Q334R L702R

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 D406A M407A F409A

Plasmid

#112108

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D406A M407A F409A

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 D406R M407R F409A

Plasmid

#112110

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D406R M407R F409A

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 Dynamin1 D406R M407R T488W

Plasmid

#112111

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D406R M407R T488W

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pF713-pEGFP-N1-Hs-FL-METTL20 wt

Plasmid

#85114

Purpose

transfection of HeLa cell for transient expression of human full-length METTL20-EGFP fusion

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human full-length METTL20 (METTL20 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-VAPA(1-242) K87D/M89D

Plasmid

#18875

Depositor

Axel Brunger

Article

Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

Insert

VAP-A (Vapa Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

double point mutant K87D / M89D

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pEGFP-N1 Dynamin1 T78R L84R T92R V118R

Plasmid

#112106

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T78R L84R T92R V118R

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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