Addgene

We narrowed to 4,075 results for: PRS

Showing: 181 - 200 of 4075 results

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pRS314_U6_A34G_A35G

Plasmid

#81232

Purpose

U6 telestem mutant (A34G/A35G) for in vivo studies

Depositor

Aaron Hoskins

Article

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with A34G/A35G mutation (snR6 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

U6 telestem mutant (A34G/A35G)

Promoter

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314_U6_G96C_A97C

Plasmid

#81235

Purpose

U6 telestem mutant (G96C/A97C) for in vivo studies

Depositor

Aaron Hoskins

Article

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with G96C/A97C mutation (snR6 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

U6 telestem mutant (G96C/A97C)

Promoter

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314_U6_U100C_U101C

Plasmid

#81236

Purpose

U6 telestem mutant (U100C/U101C) for in vivo studies

Depositor

Aaron Hoskins

Article

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with U100C_U101C mutation (snR6 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

U6 telestem mutant (G96C/A97C)

Promoter

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRSM41

Plasmid

#113940

Purpose

Constitutive bacterial expression of red fluorescent Ca2+ sensor REX-GECO1

Depositor

Thomas Hughes, Robert Campbell

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

REX-GECO1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pRS315Nab3Q->E

Plasmid

#110538

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

O'Rourke et al PLoS One. 2016 Mar 8;11(3):e0150865. doi

Insert

NAB3 (NAB3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Q to E mutations in Nab3 low complexity domain

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRS316Nab3-11

Plasmid

#110535

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

Insert

NAB3 (NAB3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

two missense mutations creating Nab3-11

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRS315Nab3D191stop

Plasmid

#110537

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

Loya et al PLoS One. 2017 Oct 12;12(10):e0186187. d

Insert

NAB3 (NAB3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

C-terminal deltion of 191 amino acids

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRS315Nab3-11

Plasmid

#110534

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

Insert

NAB3 (NAB3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

two missense mutations creating Nab3-11

Promoter

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRSM4

Plasmid

#101642

Purpose

Encodes the fluorescent protein KCyG4219 for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

KCyG4219

Use

Tags

6XHis

Expression

Bacterial

Mutation

C-terminal residues (221-223) deletion. Ser220Val…

Promoter

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pRSM13

Plasmid

#101644

Purpose

Encodes the fluorescent protein meleCFP for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

meleCFP

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pRS306Sup35R1+4

Plasmid

#17717

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

Sup35R1+4 (SUP35 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pRS306Sup35R1+5

Plasmid

#17718

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

Sup35R1+5 (SUP35 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pRS306Sup35R1+6

Plasmid

#17719

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

Sup35R1+6 (SUP35 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pRS306Sup35R1+7

Plasmid

#17720

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

Sup35R1+7 (SUP35 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pRS306Sup35R1+8

Plasmid

#17721

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

Sup35R1+8 (SUP35 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pRSV-Rev

Plasmid

#12253

Purpose

3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)

Depositor

Didier Trono

Article

Dull et al J Virol. 1998 Nov . 72(11):8463-71.

Insert

Rev (rev )

Use

Lentiviral; Packaging

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pRSETB/mStayGold

Plasmid

#212017

Purpose

mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

mStayGold

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET-MagLOV

Plasmid

#219957

Purpose

Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

Depositor

Andrew York

Article

Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

Insert

MagLOV

Use

Tags

6xHis

Expression

Bacterial

Mutation

AsLOV2 C450P L496V Q513K G528K D540M

Promoter

T7

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_DNAseI

Plasmid

#182408

Purpose

Bacterial expression of DNaseI

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

DNase I (DNASE1 Bovine)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSII42H-TDH3pr

Plasmid

#194533

Purpose

High copy vector backbone with hygromycin B selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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