We narrowed to 4,001 results for: SPL

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pStA0::SPLc19-RBSc123

Plasmid

#171899

Purpose

Promoter SPLc19 and RBSc123 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc19 and RBSc123

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc17-RBSc123

Plasmid

#171912

Purpose

Promoter SPLc17 and RBSc123 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc17 and RBSc123

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc3-RBSc21

Plasmid

#171884

Purpose

Promoter SPLc3 and RBSc21 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc3 and RBSc21

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Spike Display_Part 5 Spacer

Plasmid

#172733

Purpose

Encodes Part 5 of HexaPro-D614G spike to be used in the Spike Display system

Depositor

IIya Finkelstein

Article

Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

Insert

SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)

Use

Synthetic Biology

Mutation

Ectodomain only (AAs 1-1208); 682-685 (furin site…

Available Since

Aug. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Spike Display_Part 4 Spacer

Plasmid

#172732

Purpose

Encodes Part 4 of HexaPro-D614G spike to be used in the Spike Display system

Depositor

IIya Finkelstein

Article

Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

Insert

SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)

Use

Synthetic Biology

Mutation

Ectodomain only (AAs 1-1208); 682-685 (furin site…

Available Since

Aug. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Spike Display_Part 3 Spacer

Plasmid

#172731

Purpose

Encodes Part 3 of HexaPro-D614G spike to be used in the Spike Display system

Depositor

IIya Finkelstein

Article

Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

Insert

SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)

Use

Synthetic Biology

Mutation

Ectodomain only (AAs 1-1208); 682-685 (furin site…

Available Since

Aug. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Spike Display_Part 1 Spacer

Plasmid

#172729

Purpose

Encodes Part 1 of HexaPro-D614G spike to be used in the Spike Display system

Depositor

IIya Finkelstein

Article

Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

Insert

SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)

Use

Synthetic Biology

Mutation

Ectodomain only (AAs 1-1208); 682-685 (furin site…

Available Since

Aug. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Spike Display_Part 1b Spacer

Plasmid

#172728

Purpose

Encodes Part 1b of HexaPro-D614G spike to be used in the Spike Display system

Depositor

IIya Finkelstein

Article

Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

Insert

SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)

Use

Synthetic Biology

Mutation

Ectodomain only (AAs 1-1208); 682-685 (furin site…

Available Since

Aug. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Spike Display_Part 1a Spacer

Plasmid

#172727

Purpose

Encodes Part 1a of HexaPro-D614G spike to be used in the Spike Display system

Depositor

IIya Finkelstein

Article

Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

Insert

SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)

Use

Synthetic Biology

Mutation

Ectodomain only (AAs 1-1208); 682-685 (furin site…

Available Since

Aug. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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ESPL1 D7.1 gRNA

Plasmid

#90682

Purpose

3rd generation lentiviral gRNA plasmid targeting human ESPL1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ESPL1 (Guide Designation D7.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-Tornado-split-nLuc

Plasmid

#212612

Purpose

For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

Tornado-CVB3-split-nLuc

Use

Luciferase

Expression

Mammalian

Mutation

C373T mutation in CVB3

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-mutTornado-split-nLuc

Plasmid

#212707

Purpose

Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

mutTornado-CVB3-split-nLuc

Mutation

Deleted the partial 3'ribozyme sequence in T…

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_SS-HA-V5-LaccID-CD4 TM_pDisplay

Plasmid

#234046

Purpose

expresses LaccID on the mammalian cell surface

Depositor

Alice Ting

Article

Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

Insert

LaccID-CD4TM

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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Split 'n coexpress CUL3-RBX1

Plasmid

#52293

Purpose

bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1

Depositor

Brenda Schulman

Article

Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

Inserts

Cul3 N-terminal domain (CUL3 Human)
Cul3 C-terminal domain (CUL3 Human)
RBX1 (Rbx1 Mouse)

Tags

GST, IRES, and TEV

Expression

Bacterial

Mutation

contains aa 1-309, contains aa 310-768, and conta…

Promoter

IRES and tac

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only
Marathon-RT pentamutant for Split-PE (pEH107)

Plasmid

#190110

Purpose

Expresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R)

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

Marathon-RT

Tags

bpNLS

Expression

Mammalian

Mutation

D14R, N26R, D74R, N116K, N197R

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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HERV-Kcon-RT for Split-PE (pJUL3120)

Plasmid

#190107

Purpose

Expresses HERV-Kcon RT (human codon optimization)

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

HERV-Kcon-RT

Tags

bpNLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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tau exon 9–12 splicing minigene

Plasmid

#121165

Purpose

Generates circular RNAs from a Tau minigene

Depositor

Stefan Stamm

Article

Welden et al Biochim Biophys Acta Mol Basis Dis. 2018

Insert

Tau exons 9–12 (MAPT Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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SpCas9 nickase for Split-PE (pJUL2497)

Plasmid

#190104

Purpose

Expresses nickase SpCas9(H840A) for Split-PE use

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

nSpCas9

Tags

XTEN-bpNLS and bpNLS

Expression

Mammalian

Mutation

H840A

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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MMLV-RT(dRH) for Split-PE (pEH50)

Plasmid

#190106

Purpose

Expresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE use

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

MMLV-RT(delta RNAseH)

Tags

bpNLS

Expression

Mammalian

Mutation

mutations from RT in PE2 and truncation of RNAse …

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBSII(KSplus)-HsBGG-WT_E

Plasmid

#146187

Purpose

Bacterial Expression of HsBGG-WT

Depositor

Elisa Izaurralde

Article

Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

Insert

HsBGG-WT

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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