We narrowed to 216 results for: YPet

1. pVH666

   Plasmid

   #169610

   Purpose

   Tier-2 vector encoding PTtgO2-driven SEAP-p2A-iRFP670, PmPGK1-driven TtgA and PmPGK1-driven mRuby2 expression (PTtgO2-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-TtgA-pA::PmPGK1-mRuby2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP and iRFP expression, PPGK-driven TtgA expression cassette and PPGK-driven YPet expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO7-PCMVmin / PPGK / PPGK

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Lyso-TORCAR

   Plasmid

   #64929

   Purpose

   Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   LAMP1-TORCAR (LAMP1 Synthetic, Human)
   

   Use

   Tags

   Cerulean, Full-length LAMP1, and YPet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lyn FAK biosensor

   Plasmid

   #78299

   Purpose

   FRET biosensor. Visualization of FAK activity at lipid rafts in live cells

   Depositor

   Yingxiao Wang

   Article

   Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

   Insert

   Lyn Fak biosensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-PM-TORCAR

   Plasmid

   #64930

   Purpose

   Plasma membrane-targeted FRET biosensor for monitoring mTORC1 kinase activity.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   Lyn-TORCAR (EIF4EBP1 Synthetic, Human)
   

   Use

   Tags

   Cerulean, N-terminal targeting sequence from Lyn …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. KRas FAK biosensor

   Plasmid

   #78301

   Purpose

   FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells

   Depositor

   Yingxiao Wang

   Article

   Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

   Insert

   KRas FAK biosensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1(+)-PM-RasAR

   Plasmid

   #205569

   Purpose

   Plasma membrane-targeted RasAR.

   Depositor

   Jin Zhang

   Article

   Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

   Insert

   PM-RasAR (RAF1 Synthetic, Human)
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. alpha catenin conformation sensor

   Plasmid

   #71709

   Purpose

   This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.

   Depositor

   Deborah Leckband

   Article

   Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

   Insert

   alpha-catenin FRET Sensor
   

   Use

   Tags

   ECFP and YPET

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-TORCAR-NLS

   Plasmid

   #64931

   Purpose

   Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   TORCAR-NLS (EIF4EBP1 Synthetic, Human)
   

   Use

   Tags

   Cerulean, Nuclear localization signal (NLS), and …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Fyn-Fyn Biosensor

   Plasmid

   #124655

   Purpose

   FRET biosensor. Fyn-tagged biosensor targeting to membrane

   Depositor

   Yingxiao Wang

   Article

   Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

   Insert

   Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. FAT FAK biosensor

    Plasmid

    #78303

    Purpose

    FRET biosensor. Visualization of FAK activity at focal adhesions in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Proc Natl Acad Sci U S A. 2013 Nov 26;11

    Insert

    FAT FAK biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

11. GEF-H1 FLARE212.pa

    Plasmid

    #134930

    Purpose

    GEF-H1 biosensor that does not have GEF activity.

    Depositor

    Klaus Hahn

    Article

    Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

    Insert

    GEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
    

    Use

    Tags

    None.

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. GEF-H1 FLARE191.pa

    Plasmid

    #134931

    Purpose

    GEF-H1 biosensor that has GEF activity.

    Depositor

    Klaus Hahn

    Article

    Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

    Insert

    GEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)
    

    Use

    Tags

    None.

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)-RasAR-NES

    Plasmid

    #205567

    Purpose

    Genetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol.

    Depositor

    Jin Zhang

    Article

    Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

    Insert

    RasAR-NES (RAF1 Synthetic, Human)
    

    Use

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. beta catenin binding mutant of alpha catenin sensor

    Plasmid

    #71711

    Purpose

    This form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctions

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    alpha-catenin Δβ-cat mutant FRET Sensor
    

    Use

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Mutation

    Deleted amino acid residues 48- 163

    Promoter

    CMV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. VBS mutant of alpha catenin sensor

    Plasmid

    #71710

    Purpose

    This form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    alpha-catenin ΔVBS FRET Sensor
    

    Use

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Mutation

    Deleted amino acid residues 316-405 in α -catenin…

    Promoter

    CMV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)-RasAR(R89L)-NES

    Plasmid

    #205568

    Purpose

    RasAR negative-control mutant plasmid. Targeted to cytosol.

    Depositor

    Jin Zhang

    Article

    Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

    Insert

    RasAR(R89L)-NES (RAF1 Synthetic, Human)
    

    Use

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Arg 89 mutated to Leu in Raf1 RBD region.

    Promoter

    CMV

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)-Golgi-RasAR

    Plasmid

    #205570

    Purpose

    Golgi membrane-targeted RasAR.

    Depositor

    Jin Zhang

    Article

    Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

    Insert

    Golgi-RasAR (RAF1, GOLGB1 Synthetic, Human)
    

    Use

    Tags

    Golgin B1 (Giantin) residues 3131-3259

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPBbsr-6080NES(Epac-sensor)

    Plasmid

    #162487

    Purpose

    hyBRET-Epac, an Epac biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    Epac-sensor (RAPGEF3 Synthetic, Human)
    

    Use

    Tags

    YPet and Turquoise2GL

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET15b-M3C

    Plasmid

    #107087

    Purpose

    M. sedula PCNA3 fused at the N-terminus of CyPet

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    M. sedula PCNA3 fused to CyPet
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET15b-S3C

    Plasmid

    #107090

    Purpose

    S. solfataricus PCNA3 fused at the N-terminus of CyPet

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    S. solfataricus PCNA3 fused to CyPet
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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